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The anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on against DMBA-induced mammary cancer in Sprague Dawley rat through the regulation of microRNA-21 expression



Abstract

Background: The new anticancer is urgently needed due to the high resistance and recurrence of breast cancer. Previous study reported that a new chalcone derivative, (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on, has a potential cytotoxicity against T47D breast cancer cell line. In this study we investigated the anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on against DMBA-induced mammary cancer in Sprague Dawley rat and its effect on microRNA-21 expression.

Methods: Twenty four female rats were divided into 6 groups. The first group, G1 received corn oil. The groups 2 to 6 (G2, G3, T1, T2, and T3) were induced by DMBA (dissolved in corn oil) 20 mg/kgBW for 5 weeks. After breast nodule observed, G2 received vehicle and G3 received tamoxifen. Whereas, T1, T2, T3 received (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on with different doses, ie. 5, 15, and 45 mg/kgBW/day for 21 days, respectively. Tumor size was measured every weeks for 21 days and on day 22, plasma and breast tissues were collected to examine the miR-21 expression by qRT-PCR and histopathological feature, respectively.

Result: The result showed that significantly decreased tumor growth (p<0.05) and better histopathological malignant grading in G3, T1, T2, T3 were observed. Moreover, significantly reduced miR-21 relative expression (p<0.05) in G3, T1 and T2 were also observed. 

Conclusion: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on has potential anticancer activity on DMBA-induced mammary cancer in Sprague Dawley rat through its activity on miR-21 expression. Hence, (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on might be a new anticancer candidate in the future.

Keywords: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on tumor growth histopathology microRNA-21 expression breast cancer

How to Cite

Ika Wahyuniari, I. A., Nyoman Arijana, I. G. K., Sriwidyani, N. P., Dewi Wiryanthini, I. A., Suwito, H., Widyarini, S., Ghufron, M., Mustofa, M., & Mubarika, S. (2017). The anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on against DMBA-induced mammary cancer in Sprague Dawley rat through the regulation of microRNA-21 expression. Bali Medical Journal, 6(3), 589–594. https://doi.org/10.15562/bmj.v6i3.697
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Ida Ayu Ika Wahyuniari
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I G K Nyoman Arijana
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Ni Putu Sriwidyani
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Ida Ayu Dewi Wiryanthini
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Hery Suwito
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Sitarina Widyarini
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Muhammad Ghufron
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Mustofa Mustofa
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Sofia Mubarika
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