Background: Liver is an organ that is the most frequently suffering injury due to poisoning chemical materials. Mancozeb (Mz) is one of the poisoning chemical materials that cause the liver injury (LI). Moringa leaf (Mo) contains antioxidant to muffle the free radical. The objective of this study is to know the effects of Mo leaf in reducing the level of LI of Wistar rat induced by Mz through antioxidant mechanism.
Method: In this experimental study, 24 Wistar rats were randomly classified. Group I was terminated in the beginning of the study as the basis of necrosis index. Group II was treated as the control group. Group III was exposure with Mz started at day 11 until day 20. Group IV was given Mo started at the day 1 until day 10 and prior to giving Mz until day 20. In the beginning and the end of the study, the blood of all rats in Group II, III, and IV was aspirated in order to measure the contents of malondialdehyde (MDA), alanine aminotransferase (ALT), glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD). The necrosis index was measured in the end of the study. The data were analyzed in unpaired t-test and linear regression.
Results: Group I and II did not experience LI. The increase of MDA level in Group IV (9.67 nmol/ml), which was lower (p=0.006) than Group III (45.05 nmol/ml). The decrease of level of GPx, CAT, and SOD in Group IV (0.07 nmol/ml; 0.48 nmol/ml; and 81.5 U/ml), which was lower (p=0.008; p=0.004; and p=0.0001) than Group III (0.23 nmol/ml; 0.86 nmol/ml; and 140.9 U/ml). The increase of necrosis index and ALT level in Group IV (4.42; 25.1 u/l) was lower (p=0.04; p=0.0001) than Group III (8.56 and 67.4 u/l). The result of multivariate test showed that the most beneficial towards the LI status was SOD.Â
Conclusion: It is proven that Mo can reduce the level of LI in Wistar rats induced by Mz through the emphasis of decrease rate of endogen antioxidant of SOD.