Background: Osteoporosis is a silent metabolic disease characterized by diminished bone mass andÂ change in bone microstructure which cause increment of fracture risk. Until now, osteoporosis stillÂ becomes one of major health problems around the world. In Indonesia, the incidence of osteoporosis
is 25%. Previous study have shown the relation between osteoporosis and IL-6 gene polymorphism at
-572G>C and -174 G>C. There are some controversies about the correlation between these
polymorphism and osteoporosis because of different result between each study. Genotype GÂ polymorphism at -572 G>C of IL-6 gene has been correlated with lower Bone mineral density (BMD)Â and Genotype G polymorphism at -174G>C of IL-6 gene has been correlated with higher BMD value.
In Indonesia, there are still no study about the association between IL-6 gene polymorphism andÂ osteoporosis. In the future this IL-6 gene polymorphism could be used as a genetic marker forÂ osteoporosis in postmenopausal woman. The objective of this study is to determine the difference of
genotype of -572G>C and -174G>C polymorphism of IL-6 gene and osteoporosis in BalineseÂ postmenopausal women.
Method: This research design is a case control study. Sample was obtainedÂ at orthopedic outpatient clinic of Sanglah General Hospital, Bali-Indonesia from June 2012 until
November 2012. The diagnosis of osteoporosis is described as BMD value with T score â‰¤ -2.5 SD
using DEXA. All sampleâ€™s peripheral blood are taken to be isolated for DNA and analyzed for IL-6Â gene polymorphism at -572G>C and -174G>C using Real Time PCR. Data obtained was analyzedÂ with chi square test using SPSS.
Results: This research found 11 osteoporosis sample from total 52Â with no difference sample characteristic between case and control (p > 0.05). Using Chi square test,
There was a significant differences between genotype -572 G>C; IL-6 gene polymorphism inÂ Balinese postmenopausal woman with osteoporosis and in Balinese postmenopausal woman withoutÂ osteoporosis (c2 = 41.85 and p = 0.001) and no significant differences between genotype -174
G>C;IL-6 polymorphism in Balinese postmenopausal woman with osteoporosis and in BalineseÂ postmenopausal woman without osteoporosis(c2 = 0.283 and p = 0.518). This study discoveredÂ significant differences between genotype -572 G>C; IL-6 gene polymorphism in BalineseÂ postmenopausal woman with osteoporosis and in Balinese postmenopausal woman withoutÂ osteoporosis.
Conclusion: We concluded that ethnic genetic profile variations affect the role of IL-6Â gene polymorphism. In the future, this IL-6 gene polymorphism could be used as risk factor for
osteoporosis in Balinese premenopausal woman