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Adipose-derived stem cells (ADSCs) impact on the formation of epidural fibrosis in a wistar rats’ experimental laminectomy


Background: Epidural fibrosis is the most prevalent cause of failed back surgery. Adipose-derived stem cell provides a possible means of regulating and preventing excessive fibroblast proliferation, stimulating tissue vascularization, and yielding an optimal environment for tissue healing. This preliminary study evaluated the role of adipose-derived stem cell administration on post-laminectomy epidural fibrosis formation, macroscopically and microscopically.

Methods: Laminectomy was performed on the thoracic and lumbar region of ten Wistar rats. The thoracic region received 10 µl of 0.9% Sodium chloride administration over the laminectomy site and was considered the control group. The lumbar region received adipose-derived stem cells with a dose of 2 x 105 cells (10 µl) and was determined as the treatment group. The rats were killed thirty days after surgery; the thoracic and lumbar columns were resected en bloc. Qualitative analysis consisting of macroscopic and microscopic examinations was carried out in the control and treatment groups.

Results: The grading of epidural fibrosis macroscopically and microscopically was lower in the treatment group with adipose-derived stem cell administration than in the control group.

Conclusion: ADSCs might reduce the formation of epidural fibrosis after the laminectomy procedure by modulating the fibroblast formation and inflammatory cytokine during the healing process.


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How to Cite

Natakusuma, T. I. S. D., Mahadewa, T. G. B. M., Putra, M. B. P., Monica, M. ., Awyono, S. ., Saapang, G. S. ., Prakoso, D. T. ., & Sriwidyani, N. P. S. (2024). Adipose-derived stem cells (ADSCs) impact on the formation of epidural fibrosis in a wistar rats’ experimental laminectomy. Bali Medical Journal, 13(3), 951–956.




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Tjokorda Istri Sri Dalem Natakusuma
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Tjokorda Gde Bagus Mahadewa Mahadewa
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Made Bhuwana Putra Putra
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Maria Monica
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Steven Awyono
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Glen Sandi Saapang
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Dicky Teguh Prakoso
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Ni Putu Sriwidyani Sriwidyani
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