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Formula characteristics and physical stability test in glutathione cream

  • Tiara Amanna Amandita ,
  • Lynda Hariani ,
  • Beta Subakti Nata’atmadja ,


Introduction: Glutathione is an antioxidant that can minimize the effects of oxidative stress by increasing intracellular glutathione and accelerate wound healing by increasing the contraction capacity of fibroblasts. Research development with topical application, especially cream, allows concentration adjustments to improve wound healing more effectively.

Method: This research method is a true experimental research using pre-post test without control group design. The glutathione cream formula was replicated 3 times, then cycling test was carried out with duration of storage period for 12 days, then observed the organoleptic, pH test, spreadability, emulsion type and viscosity cream.

 Result: Organoleptical observations of Glutathione cream before and after the cycling test were the same. pH test results with p 0.004 <0.05, which means there is difference between the pH before and after the cycling test. The viscosity test with p 0.005 <0.05 shows an average difference between viscosity before and after the cycling test. There was no significant difference between the spreadability of Glutathione cream before and after the cycling test with p 0.423 > 0.05.

Conclusion: The conclusion of this study is the characteristics of Glutathione cream after the cycling test obtained a semi-solid texture, bone-white color, and a distinctive smell of cream and distributed homogeneous, the pH of the cream decreases, which becomes acidic 4.070 ± 0.070, and the results of the spreadability of the cream did not find significant differences before and after the cycling test, and after the centrifuge test obtained a stable cream formula, no phase separation. There was no difference between the emulsion type of Glutathione cream before and after the cycling test, with the emulsion type is o/w, from the test results it can be concluded that the cream is stable.


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How to Cite

Amandita, T. A., Hariani, L. ., & Nata’atmadja, B. S. . (2024). Formula characteristics and physical stability test in glutathione cream. Bali Medical Journal, 13(3), 961–966.




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Tiara Amanna Amandita
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Lynda Hariani
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Beta Subakti Nata’atmadja
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