Skip to main content Skip to main navigation menu Skip to site footer

Diagnostic value of immature platelet fraction for detecting production disorders in thrombocytopenia

  • I Wayan Losen Adnyana ,
  • Ni Made Renny Anggreni Rena ,
  • Nyoman Martha Chrismayana ,
  • Putu Gizha Satria Gautama ,

Abstract

Link of Video Abstract: https://youtu.be/FrXavnzSY3Q

 

Introduction: Impaired platelet production is one of the causes of thrombocytopenia. Immature platelet fraction (IPF) shows the proportion of young platelets in peripheral blood. A decrease in the number of young platelets reflects a decrease in the thrombopoiesis process. This suggests that young platelets can be a marker for measuring megakaryopoiesis activity. This study aimed to determine the diagnostic value of IPF.

Methods: The sample will be divided into two groups; groups suspected of production disturbances and groups not suspected of production disturbances. In the group with suspected production disorders, bone marrow aspiration (BMA) will be carried out to determine a definitive diagnosis. Meanwhile, in the group not suspected of production disruption, bias work-up will be carried out. After that, descriptive statistical analysis, ROC analysis and diagnostic tests will be carried out.

Result: This study used 81 cases of thrombocytopenia with a proportion of 49 (60.5%) in the production disorder group and 32 (39.5%) in the non-production disorder group. The IPF% value in the production disruption group is significantly lower than the non-production disruption group 3.1% (0.3-21.4) vs 13% (2.0-49.2) p value <0.001. The results of calculations using ROC analysis in this study showed an AUC of 0.917 (95% confidence interval (CI): 0.853-0.981, p<0.001). The results of the analysis set a cut-off value of 7.5% at sensitivity 83.7%, specificity 84.4%, PPV 89.1%, NPV 77.1%, LR+ 5.53, LR- 0.19, prevalence 60.4%, and 83.9% accuracy.

Conclusion: The results of this study showed that IPF% AUC was 0.917, indicating that IPF% was very good for distinguishing production disorders from theombocytopenia with an accuracy of 83.9%.

References

  1. Hoffbrand AV, Moss PAH, Pettit JE. Bleeding disorders caused by vascular and platelet abnormalities. In: Essential haematology. 7thed. London Blackwell Science. 2016;279-89.
  2. Moulis G, Christiansen CF, Darvalics B, Nørgaard M. Prevalence of thrombocytopenia and thrombocytosis upon acute hospital admission to internal medicine units. A cross-sectional study in Denmark. EJIM. 2018;57:34-7.
  3. Brace LD. Thrombocytopenia and thrombocytosis. In: Rodak BF, Fritsma GA, editors. Hematology clinical principles and applications. Missouri-Saunders. 2012;4:694-713.
  4. Margareth D. Gambaran nilai Immature Platelet Fraction pada pasien dengan trombositopenia di Departmen Penyakit Dalam RSUP Haji Adam Malik Medan. Repositori Institusi Universitas Sumatera Utara. 2019;25-31.
  5. Harrison P, Robinson MS, Mackie IJ, Machin SJ. Reticulated platelets. Platelets. 1997;8(6):379–83.
  6. Jimenez MM, Guedan MJ, Martin LM, Campos JA, Martinez IR, Vilella CT. Measurement of reticulated platelets by simple flow cytometry: an indirect thrombocytopoietic marker. Eur J Intern Med. 2006;17(8):541–4.
  7. Briggs C, Longair I, Kumar P, Singh D, Machin SJ. Performance evaluation of the Sysmex haematology XN modular system. J Clin Pathol. 2012;65(11):1024–30.
  8. Feng G, Gale RP, Cui W, Cai W, Huang G, Xu Z, et al. A systematic classification of megakaryocytic dysplasia and its impact on prognosis for patients with myelodysplastic syndromes. Exp Hematol Oncol. 2016;5:12.
  9. Abe Y, Wada H, Tomatsu H, Sakaguchi A, Nishioka J, Yabu Y, et al. A simple technique to determine thrombopoiesis level using immature platelet fraction (IPF). Thromb Res. 2006;118(4):463–9.
  10. Van De Wyngaert Z, Fournier E, Bera E, Carrette M, Soenen V, Gauthier J, et al. Immature platelet fraction (IPF): A reliable tool to predict peripheral thrombocytopenia. Curr Res Transl Med. 2020;68:37-42.
  11. Jeon K, Kim M, Lee J, Lee JS, Kim HS, Kang HJ, et al. Immature platelet fraction: A useful marker for identifying the cause of thrombocytopenia and predicting platelet recovery. Medicine (Baltimore). 2020;99(7):e19096.
  12. Jung H, Jeon HK, Kim HJ, Kim SH. Immature platelet fraction: establishment of a reference interval and diagnostic measure for thrombocytopenia. Korean J Lab Med. 2010;30(5):451–62.

How to Cite

Adnyana, I. W. L., Rena, N. M. R. A. ., Chrismayana, N. M., & Putu Gizha Satria Gautama. (2023). Diagnostic value of immature platelet fraction for detecting production disorders in thrombocytopenia. Bali Medical Journal, 12(3), 3105–3107. https://doi.org/10.15562/bmj.v12i3.4860

HTML
9

Total
2

Share

Search Panel

I Wayan Losen Adnyana
Google Scholar
Pubmed
BMJ Journal


Ni Made Renny Anggreni Rena
Google Scholar
Pubmed
BMJ Journal


Nyoman Martha Chrismayana
Google Scholar
Pubmed
BMJ Journal


Putu Gizha Satria Gautama
Google Scholar
Pubmed
BMJ Journal