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Soursop leaves (Annona muricata Linn) ethanol extract prevents cisplatin-induced kidney injury of mice (Mus musculus) through SOD and MDA analysis

  • I Made Sudanta ,
  • Djoko Santoso ,
  • I Ketut Sudiana ,
  • Siswandono ,
  • pdf  |
  • Published: 2023-09-03


Abstract

Background: Cancer treatment has been carried out in various ways, including surgery, chemotherapy and immunotherapy. Cisplatin is often used for chemotherapy, but the use of cisplatin still has extensive side effects, including kidney injury. The ethanol extract of soursop leaves contains antioxidants expected to reduce ROS or induce SOD, glutathione and catalase, thereby preventing kidney injury. This study aims to explain the effect of soursop leaves ethanol extract on preventing kidney injury caused by cisplatin administration in animal models.

Method: This study is a true experimental study with a randomized, posttest-only control group design. We used 40 mice and randomly divided them into 5 groups, each group containing 8 mice.  The K0 group was given aquadest, the K1 group was given cisplatin 20 mg/kgBW, the KP1 group was given cisplatin and soursop leave ethanol extract 100 mg/kgBW, the KP2 group was given cisplatin and soursop leave ethanol extract 200 mg/kgBW, the KP3 group given cisplatin and soursop leaves ethanol extract 400mg/kgBW. The extract was given orally for 5 days in a row. MDA and F2-isoprostane were measured using Immunohistochemistry. The data were all analyzed using an ANOVA test.

Results: Soursop leaves ethanol extract with 200 mg/kgBW is the most effective dose to prevent kidney injury from cisplatin exposure. Soursop leaves ethanol extract can increase the expression of SOD and decrease the MDA metabolites.

Conclusion: Soursop leaves ethanol extract can prevent kidney injury by increasing SOD and decreasing MDA.

Keywords: kidney injury cisplatin soursop extract MDA SOD

References

  1. Riskesdas. Laporan Hasil Riset Kesehatan Dasar. Riskesdas. [Internet]. 2018. [cited 2023 July 12]. Available from: http://www.depkes.go.id/resources/download/info-terkini/hasil-riskesdas-2018.pdf.
  2. Global Burden of Cancer Study. Cancer today. International Agency for Research on Cancer. [Internet]. 2020. [cited 2023 July 12]. Available from: https://gco.iarc.fr/
  3. Della Zulfa Rifda, Zahroh Shaluhiyah, & Antono Surjoputro. Studi Fenomenologi Pasien Kanker Payudara dalam Upaya Meningkatkan Kualitas Hidup : Literature Review. Media Publikasi Promosi Kesehatan Indonesia (MPPKI). 2023; 6(8), 1495–1500. doi: https://doi.org/10.56338/mppki.v6i8.3513
  4. Irawati, I., & Sardjan, M. Pola Peresepan Obat Kemoterapi Kanker Payudara di Rumah Sakit Lavalette Kota Malang. PHARMADEMICA : Jurnal Kefarmasian Dan Gizi, 1(2),2022; 80–85. Doi: https://doi.org/10.54445/pharmademica.v1i2.12
  5. Suwendar, S., Fudholi, A., Andayani, T. M., & Sastramihardja, H. S. Humanistic Outcome of Cervical Cancer Patients Using Cisplatin-Vincristine-Bleomycin Regimen. Indonesian Journal of Clinical Pharmacy. 2021;10(4), 249–260. Doi: https://doi.org/10.15416/ijcp.2021.10.4.249
  6. Lirans TK, Dwi A and Muyassaroh. Hubungan Lama Waktu Pasca Kemoradiasi dengan Derajat Disfagia Orofaringeal pada Karsinoma Nasofaring. Medica Hospitalia : Journal of Clinical Medicine, 2021;8(1).
  7. Made Violin Weda Yani, K., Ayu Widya Anjani, I., Setula Narayana, G., Made Wihandani, D., & Gede Putu Supadmanaba, I. Combination of Cisplatin-Withaferin Based on PEGylated Liposome Nanoparticles as Alternative Therapy for Ovarian Cancer. Journal of Medicine and Health. 2020;2(5).
  8. Sudiana IK. Patobiologi Molekuler Kanker. Salemba Medika:Jakarta. 2011.
  9. Suranto A. Dahsyatnya Sirsak Tumpas Penyakit. Pustaka Bunda: Jakarta. 2011.
  10. Bhardwaj, R., Pareek, S., Sagar, N. A., and Vyas, N. Bioactive Compounds of Annona. Reference Series in Phytochemistry. 2019;1–26. https://doi.org/10.1007/978-3-030-06120-3_5-1
  11. Dwi, N. G. A. M., Suastuti, A., Sri, G. A. K., Dewi, P., Ni, D., and Ariati, K. Pemberian ekstrak daun sirsak (Annona muricuta) untuk memperbaiki kerusakan sel beta pankreas melalui penurunan kadar glukosa darah, advanced glycation and product dan 8-hidroksi-2-dioksiguanosin pada tikus wistar hiperglikemia. Jurnal Kimia. 2015; 9(2):289-295.
  12. Palawe, C. Y., Kairupan, C. F., & Lintong, P. M. Efek Hepatoprotektif Tanaman Obat. Medical Scope Journal. 2021;3(1):61. DOI: https://doi.org/10.35790/msj.3.1.2021.33542
  13. Murray, R. K., Granner, D.K., Mayes, P.A. and Rodwell., V.W. Biokimia Harper. Jakarta: EGC. 2014.
  14. Naiborhu, PE. Ekstraksi dan manfaat ekstrak mangrove (Sonneratia alba dan Sonneratia caesolaris) sebagai bahan alami antibakterialpada patogen udang windu, Vibrio harveyi [Tesis]. Bogor: Institut Pertanian Bogor. 2002.
  15. Mudasir A, Azis A, Punagi Q. Analisis Kadar MDA Plasma Penderita Polip Hidung Berdasarkan Dominasi Sel Inflamasi Pada Pemeriksaan Histopatologi. [Thesis] Makasar: Fakultas Kedokteran Universitas Hasanuddin. 2011.
  16. Hazra, B., Sarkar, R., Biswas, S., & Mandal, N. Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis. [Internet]. BMC Complementary and Alternative Medicine. 2010 [cited 2022 July 12]. Available from: http://www.biomedcentral.com/1472-6882/10/20.
  17. Tokur, B., Korkmaz, K., & Ayas, D. Comparison of Two Thiobarbituric Acid (TBA) Method for Monitoring Lipid Oxidation in Fish. In E.U. Journal of Fisheries & Aquatic Sciences. 2006;23(4)
  18. Fang, C. yan, Lou, D. yong, Zhou, L. qin, Wang, J. cheng, Yang, B., He, Q. jun, Wang, J. jia, & Weng, Q. jie. Natural products: potential treatments for cisplatin-induced nephrotoxicity. Acta Pharmacologica Sinica. 2021;42(12);1951–1969. Doi: https://doi.org/10.1038/s41401-021-00620-9
  19. Solfaine, R., Putri, M., Supriyanto, E., & Surabaya, K. Gambaran Histopatologi Ginjal yang Diinduksi Cisplatin dan Ekstrak Daun Torbangun (Coleus ambonicus, L.) pada Tikus Wistar (Rattus norvegicus) Effect of Torbangun Leaf (Coleus ambonicus, L.) Extract Feeding on Cisplatin-Induced in Wistar Rats (Rattus norvegicus). Vet Bio Clin J. 2022;4(1).
  20. Oh, G. S., Kim, H. J., Shen, A. H., Lee, S. bin, Khadka, D., Pandit, A., & So, H. S. Cisplatin-induced kidney dysfunction and perspectives on improving treatment strategies. Electrolyte and Blood Pressure. 2014;12(2):55–65. Doi: https://doi.org/10.5049/EBP.2014.12.2.55.
  21. Tazkia R.R, Amalia Y, Damayanti D.S, Efek Ekstrak Air daun Sirsak (Annona Muricata) Terhadap Kadar SOD dan MDA Hepar Tikus Wistar Induksi Diet Tinggi Lemak Dan Tinggi Fruktosa. Jurnal Bio Komplementer medicine. 2019;6(3):1-7.
  22. Yoni Astuti, Agus Suharto, Sabtanti Harimurti, Helmi Hana Prinanda, Wahyu Joko Priambodo. Number of e-cadherin and VEGF in WiDr cell line post-induced by extract ethanol Annona muricata leaf. Bali Med J. [Internet]. 2023 Feb. 2 [cited 2023 Aug. 30];12(1):524-9. Available from: https://www.balimedicaljournal.org/index.php/bmj/article/view/3736.
  23. Astuti Y, Priambodo WJ, Rahmah, Septiyana DA. Overview of wound assay methods extract ethanol leaves soursop (annona muricata) on widr colon cancer cells. Bali Med J. [Internet]. 2021 Dec. 30 [cited 2023 Aug. 30];10(3):1385-9. Available from: https://www.balimedicaljournal.org/index.php/bmj/article/view/2979.
  24. Okafor IA, Nnamah US, Nnaka J. Lipid and cardiac profile assessment following the co-administration of Cisplatin and methanolic extract of Portulaca oleracea in adult Wistar rats: an experimental study. Bali Med J. [Internet]. 2020 Dec. 1 [cited 2023 Aug. 30];9(3):799-805. Available from: https://www.balimedicaljournal.org/index.php/bmj/article/view/1832.

How to Cite

I Made Sudanta, Djoko Santoso, Sudiana, I. K., & Siswandono. (2023). Soursop leaves (Annona muricata Linn) ethanol extract prevents cisplatin-induced kidney injury of mice (Mus musculus) through SOD and MDA analysis. Bali Medical Journal, 12(3), 2619–2622. Retrieved from https://balimedicaljournal.ejournals.ca/index.php/bmj/article/view/4760
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