Skip to main content Skip to main navigation menu Skip to site footer

Characterization of ultraviolet B light emitting diodes (UVB LED) irradiation device for Wistar rats as an experimental animal model

  • Diah ,
  • Chiquita Prahasanti ,
  • Retno Puji Rahayu ,
  • Vani Rachmad Wijayanto ,


Link of Video Abstract:


Background: Light emitting diodes (LED) lamps are used widely in daily because of their many benefits, including their low cost, extended lifespan, low energy consumption, and low environmental impact. The use of LEDs, especially ultraviolet B LED (UVB LEDs) is quite extensive and has been developed for the treatment of skin diseases, irradiating pet reptiles, and conducting research on experimental animals. The use of light for therapy must be done carefully so that unwanted side effects do not occur because light also harms tissue. The purpose of this characterization is to determine the stability of temperature and irradiation of the device we make and to determine the duration of light exposure based on the characterization.

Methods: The irradiation device use UVB LEDs with a wavelength of 305-310 nm. The characterization carried out included temperature stability and irradiation stability, with observations every 30 minutes for 10 hours and no replication. Statistical analysis using the Kolmogorov-Smirnov (Asymptotic Approximation) testing technique. The test criteria state that if the probability value > level of significance (alpha (α) = 5%).

Results: The characterization results from 10 hours of observation with sampling data taken every 30 minutes showed stable LED temperature and room temperature while unstable cage temperature and irradiance.

Conclusion: This device can be considered to use in Wistar rat’ experimental studies using UVB and perhaps developed further. The duration of exposure can be adjusted according to the distance of the object and the dose required.


  1. Bergesen JD, Tähkämö L, Gibon T, Suh S. Potential long-term global environmental implications of efficient light-source technologies. J Ind Ecol. 2016;20(2):263-75.
  2. Austin E, Geisler AN, Nguyen J, Kohli I, Hamzavi I, Lim HW, et al. Visible light. Part I: Properties and cutaneous effects of visible light. J Am Acad Dermatol. 2021;84(5):1219-31.
  3. Schubert FE. Light Emitting Diodes. 3rd ed. E. Fred Schubert; 2018.
  4. Oh ST, Park DH, Lim JH. Designing safe general LED lighting that provides the UVB benefits of sunlight. Appl Sci. 2019;9(5).
  5. Kalajian TA, Aldoukhi A, Veronikis AJ, Persons K, Holick MF. Ultraviolet B light emitting diodes (LEDs) are more efficient and effective in producing vitamin D3 in human skin compared to natural sunlight. Sci Rep. 2017;7(1):6-13.
  6. Orlova T, Moan J, Lagunova Z, Aksnes L, Terenetskaya I, Juzeniene A. Increase in serum 25-hydroxyvitamin-D3 in humans after sunbed exposures compared to previtamin D3 synthesis in vitro. J Photochem Photobiol B Biol. 2013;122:32-36.
  7. Barros N de M, Sbroglio LL, Buffara M de O, Baka JLC e. S, Pessoa A de S, Azulay-Abulafia L. Phototherapy. An Bras Dermatol. 2021;96(4):397-407.
  8. Pirc M, Caserman S, Ferk P, Topič M. Compact uv led lamp with low heat emissions for biological research applications. Electron. 2019;8(3).
  9. Hart PH, Norval M. More than effects in skin: ultraviolet radiation-induced changes in immune cells in human blood. Front Immunol. 2021;12:6-12.
  10. Ye J, Huang H, Luo G, Yin L, Li B, Chen S, et al. NB-UVB irradiation attenuates inflammatory response in psoriasis. Dermatol Ther. 2020;33(4):1-6.
  11. Farid A, Tawfik A, Elsioufy B, Safwat G. Narrow band ultraviolet B therapy deactivates Th1/Th17 pathway and activates Th2 cytokines secretion in Egyptian psoriatic arthritis patients. J Radiat Res Appl Sci. 2020;13(1):356-61.
  12. Morita D, Nishida Y, Higuchi Y, Seki T, Ikuta K, Asano H, et al. Short-range ultraviolet irradiation with LED device effectively increases serum levels of 25(OH)D. J Photochem Photobiol B Biol. 2016;164:256-63.
  13. Barnkob LL, Argyraki A, Petersen PM, Jakobsen J. Investigation of the effect of UV-LED exposure conditions on the production of Vitamin D in pig skin. Food Chem. 2016;212:386-91.
  14. Lin MY, Lim LM, Tsai SP, Jian FX, Hwang SJ, Lin YH, et al. Low dose ultraviolet B irradiation at 308 nm with light-emitting diode device effectively increases serum levels of 25(OH)D. Sci Rep. 2021;11(1):1-9.
  15. Guo R, Du Y, Zhang S, Liu H, Fu Y. The effects of ultraviolet supplementation to the artificial lighting on rats’ bone metabolism, bone mineral density, and skin. J Photochem Photobiol B Biol. 2018;188(37):12-18.
  16. Ochiai S, Nishida Y, Higuchi Y, Morita D, Makida K, Seki T, et al. Short-range UV-LED irradiation in postmenopausal osteoporosis using ovariectomized mice. Sci Rep. 2021;11(1):1-13.
  17. Myers E, Kheradmand S, Miller R. An Update on narrowband ultraviolet B therapy for the treatment of skin diseases. Cureus. 2021;13(11):1-7.
  18. Yoshimura T, Manabe C, Inokuchi Y, Mutou C, Nagahama T, Murakami S. Protective effect of taurine on UVB-induced skin aging in hairless mice. Biomed Pharmacother. 2021;141:111898.
  19. Moshammer H, Simic S, Haluza D. UV “Indices”—what do they indicate?. Int J Environ Res Public Health. 2016;13(10):1041.
  20. Oliveira LMC, Tuchin VV. The Optical Clearing Method. Springer Nature; 2019. doi:10.1007/978-3-030-33055-2
  21. Orlova T, Terenetskaya I. UV phototherapy : a new look at the UV sources and doses. Clin Med. 2020;2(1):5-8.
  22. Li T, Lin F, Ji M, Huang B, Cheng W, Shi L, et al. Development and measurement of a 365 NM UV LED irradiance source. Meas Sensors. 2021;18:100153.
  23. Hoque MA, Bradley RK, Fan J, Fan X. Effects of humidity and phosphor on silicone/phosphor composite in white light-emitting diode package. J Mater Sci Mater Electron. 2019;30(23):20471-8.
  24. Zibold A, Dammann M, Schmidt R, Konstanzer H, Kunzer M. Influence of air pollutants on the lifetime of LEDs and analysis of degradation effects. Microelectron Reliab. 2017;76-77:566-70.
  25. Rammohan A, RameshKumar C. Investigation on light intensity and temperature distribution of Automotive’s Halogen and LED headlight. 2017 Int Conf Microelectron Devices, Circuits Syst ICMDCS 2017. 2017;2017:1-6.

How to Cite

Diah, Prahasanti, C. ., Rahayu, R. P., & Wijayanto, V. R. (2023). Characterization of ultraviolet B light emitting diodes (UVB LED) irradiation device for Wistar rats as an experimental animal model. Bali Medical Journal, 12(3), 2516–2520.




Search Panel