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Skin response to lipopolysaccharide-induced sepsis based on histology

  • Maria Ulfa ,
  • Hotimah Masdan Salim ,
  • Winawati Eka Putri ,
  • Irmawan Farindra ,


Introduction: The skin is the largest organ of the human body. Sepsis is a serious disease and causes multi-organ damage, with a high cause of death. however, the mechanism by which sepsis can damage the skin structure is not clear. This study aimed to investigate skin damage in a lipopolysaccharide-induced endotoxemia (LPS) model widely used in mice.

Methods: This study used an experimental design with a control group that was randomized solely for the post-test. White mice (Mus musculus), the study's population, made up this group. LPS injection for 4 hours (LPS+4h, n=4), LPS injection for 8 hours (LPS+8h, n=4), and control group (Ctrl group, n=4) were randomly assigned to mice. The SPSS ver. 25 statistical analysis program was used. One-Way ANOVA was used to compare more than three sets of data, and Tukey's multiple comparison test was used to assess the results. The p-value of 0.05 was used to determine if the difference was significant.

Results: Based on this study, LPS injection increased the leukocyte concentration significantly (p-value<0.05) in the 4 h and 8 h vs control group. LPS-induced sepsis decreased body weight significantly (p-value<0.05). The morphology of skin thickness in the control group was normal, according to the results of the histopathologic study of the area stained with hematoxylin and eosin. However, the thickness was decreased in mice after 4 hours and 8 injections of LPS significantly (p-value<0.05).

Conclusion: LPS-induced septic mice cause damage to the skin, and changes in skin thickness due to the inflammatory process due to sepsis.


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How to Cite

Maria Ulfa, Hotimah Masdan Salim, Winawati Eka Putri, & Irmawan Farindra. (2023). Skin response to lipopolysaccharide-induced sepsis based on histology. Bali Medical Journal, 12(3), 3251–3253.




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