Skip to main content Skip to main navigation menu Skip to site footer

Hepatoprotective effect of ethanolic extract of Syzygium polyanthum in rats induced by rifampicin and isoniazid

  • Handayani ,
  • Renny Novi Puspitasari ,
  • Lysa Veterini ,
  • Devyana Dyah Wulandari ,


Introduction: Syzygium polyanthum (Bay leaf) is one of the species of the family Myrtaceae used as a seasoning and medicine, especially in Southeast Asia such as Malaysia and Indonesia. This leaf is known to have medicinal properties to prevent cholesterol, diabetes mellitus, hypertension, gastritis, diarrhea, and gout. This study evaluates the hepatoprotective properties of the ethanolic extract of Syzygium polyanthum in rats induced by rifampicin and isoniazid.

Methods: These hepatoprotective properties were evaluated by the assay of liver function parameters, such as total bilirubin, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), the histopathological study of the liver and malondialdehyde (MDA) as a marker of stress oxidative.

Results: Administration of ethanolic extract of Syzygium polyanthum has no significant effect (P>0,05) on total bilirubin, SGOT, and SGPT, but can reduce MDA descriptively. Ethanolic extract of Syzygium polyanthum could not cure the liver tissue of rats in histopathology examination. It means that ethanolic extract of Syzygium polyanthum can help to reduce oxidative stress in liver tissue.

Conclusion: In further research, it is recommended to develop other bioactivity tests from bay leaf isolates and conduct more specific studies to explain the mechanism of action of the extract at the cellular and organismal levels that are more complex, as well as toxicity tests to determine pharmacological effects.


  1. Amang A. P., Kodji, E., Mezui,C., Baane, M. P., Siwe, G. T., Kuissu, T. M., Emakoua, J., and P. V. Tan. 2020. Hepatoprotective effects of aqueous extract of opilia celtidifolia (opiliaceae) leaves against ethanol-induced liver damage in rats. Hindawi Evidence-Based Complementary and Alternative Medicine, 10, 1-8
  2. Ali, M. H., Alrasheedy, A. A., Kibuule, D., Hassali, M. A., Godman, B., Abdelwahab, M. F., & Abbadi, R. Y. 2019. Isoniazid acetylation phenotypes in the Sudanese population, findings and implications. Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, 17
  3. Batool, S., Khera, R. A., Hanif, M. A., & Ayub, M. A. Bay leaf. 2020. Medicinal Plants of South Asia, 9, 63–74
  4. Biswas, A. et all. 2020. Isoniazid and rifampicin produce hepatic fibrosis through an oxidative stress-dependent mechanism. International Journal of Hepatology, 4, 1-12
  5. Dewijanti, I.D., Mangunwardoyo, W., Dwiranti, A., Hanafi, A., Artanti, N. 2020. Short communication: Effects of the various source areas of Indonesian bay leaves (Syzygium polyanthum) on chemical content and antidiabetic activity. BIODIVERSITAS, 21(3), 1190-1195
  6. Djabir, Y.Y., Meylin, I.d., Tayeb, R. 2019. The protective effect of paliasa (kleinhovia hospita l.) leaf extract against elevated total bilirubin serum induced by toxic dose of antituberculosis in rats. Nusantara Medical Science Journal, 4(2), 1-5
  7. Gillessen, A., & Schmidt, H. H. 2020. Silymarin as supportive treatment in liver diseases: a narrative review. Advances in therapy, 37(4), 1279–1301
  8. Hidayati, W., Sjahid, L.R., Ismalasari, W., Kusmardi, K. 2020. The potency of 96% ethanol extract of indonesian bay leaves (syzigium polyanthumwight. (walp.) on p53 expression on hela cell lines. Jurnal Kefarmasian Indonesia, 10(2), 79-86
  9. Lei, S., Gu, R., Ma, X. 2021. Clinical perspectives of isoniazid-induced liver injury. Liver Research, 5(2), 45-52
  10. Luliana, S., Riza, H., Indriyani, E.N. 2019. The Effect of Extraction Method on Total Phenolic Content andAntioxidant Activity of Salam Leaves (Syzygium polyanthum) usingDPPH (1,1-Diphenyl-2-Picrylhidrazil). Trad. Med. J, 24(2), 72-76
  11. MacNeil A,, Glaziou P,, Sismanidis C,, Date A,, Maloney S,, Floyd K. 2020. Global epidemiology of tuberculosis and progress toward meeting global targets worldwide, MMWR Morb Mortal Wkly Rep, 69(11), 281–285
  12. Ndrepepa, G. 2021. Aspartate aminotransferase and cardiovascular disease a narrative review. J Lab Precis Med, 6(1), 1-17
  13. Parés A., Shiffman M., Vargas V., Invernizzi P., Malecha E.S., Liberman A., MacConell L., Hirschfield G. 2020. Reduction and stabilization of bilirubin with obeticholic acid treatment in patients with primary biliary cholangitis. Liver Int., 40(5), 1121-1129
  14. Pennington, K. M., Kennedy, C. C., Chandra, S., Lauzardo, M., Brito, M. O., Griffith, D. E., Seaworth, B. J., & Escalante, P. 2018. Management and diagnosis of tuberculosis in solid organ transplant candidates and recipients: Expert survey and updated review. Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, 11, 37–46
  15. Sahlan, M., Hapsari, N.R.A., Pratami, K.D., Khayrani, A.C., Lischer, K., Alhazmi, A. 2021. Mohammedsaleh, Z.M., Shater, A.F., Saleh, F.M., Alsanie, W.F., Sayed, S., Gaber, A. Potential hepatoprotective effects of flavonoids contained in propolis from South Sulawesi against chemotherapy agents, Saudi Journal of Biological Sciences, 28(10), 1-8
  16. Sembiring, D., Levita, J. Hepatotoxicity and hepatoprotective activities of plant malvaviscus. 2020. Farmaka, 17(3), 142-150
  17. Soedarsono,, Riadi, A.R.W. 2020. Tuberculosis Drug-Induced Liver Injury. JURNAL RESPIRASI, 06(02), 49-54
  18. Tungmunnithum, D., Thongboonyou, A., Pholboon, A., & Yangsabai, A. 2018. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: an overview. Medicines (basel, switzerland), 5(3), 93-109
  19. Vairetti, M., Di Pasqua, L.G., Cagna, M., Richelmi, P., Ferrigno, A., Berardo, C. 2021. Changes in Glutathione Content in Liver Diseases: An Update. Antioxidants, 10(3), 364-403
  20. Wulandari, D.D., Mufidah, Z., Ersam, T., Wulansari, D.D., Corpuz, A.V. 2020. Alpha mangosteen effect on MDA level and the pancreatic morphology rattus norvegicus (Berkenhout, 1769) induced by alloxan. Proceedings of the Pakistan Academy of Sciences: Pakistan Academy of Sciences B. Life and Environmental Sciences. 57: 13-19

How to Cite

Handayani, Renny Novi Puspitasari, Lysa Veterini, & Devyana Dyah Wulandari. (2023). Hepatoprotective effect of ethanolic extract of Syzygium polyanthum in rats induced by rifampicin and isoniazid. Bali Medical Journal, 12(3), 3387–3391.




Search Panel

Google Scholar
BMJ Journal

Renny Novi Puspitasari
Google Scholar
BMJ Journal

Lysa Veterini
Google Scholar
BMJ Journal

Devyana Dyah Wulandari
Google Scholar
BMJ Journal