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Effectiveness of topical turmeric extract compared to topical zinc on fibroblast count and collagen density in clean-contaminated wound: a study on new zealand white rabbit

  • Made Walmiky Budi ,
  • Adria Hariastawa ,
  • Fendy Matulatan ,


Introduction: Wound dehiscence increased the average mortality and morbidity, patient care cost and health services. Therefore, a more effective and lower-cost therapy is needed for wound healing. Zinc is a trace element which is important in the wound-healing process, while curcumin is an active component of turmeric which can also help the wound-healing process. This study aims to compare the effectiveness of topical turmeric extract with topical zinc on fibroblast count and collagen density in clean-contaminated wounds in New Zealand white rabbits.

Material and Methods: This study was a randomized experimental study in 30 New Zealand white rabbits with clean contaminated wounds divided into 3 groups. One control group (treated with NaCl 0.9%) and two treatment groups (treated with topical turmeric extract and with topical zinc). Appendectomy was performed to all samples and surgical wounds were treated until day 4 postoperative. On the fifth day, histopathology observation at the edge of the surgical wound was done to see the fibroblast count using Haematoxylin Eosin staining and collagen density using Masson Trichrome staining.

Results: There was a significant difference in the number of fibroblasts between the control group (NaCl 0.9%), the topical zinc group and the topical turmeric extract group (Kruskal-Wallis p=0.000). The collagen density score also had significant differences in the three groups (Kruskal-Wallis p=0.003). Although the Mann-Whitney test in the topical zinc group and the topical turmeric extract group showed no significant difference in the collagen density score (p=0.074).

Conclusion: Topical administration of turmeric extract has been shown to affect the number of fibroblasts and collagen density in clean-contaminated wounds in New Zealand white rabbits, as well as topical zinc administration.


  1. Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci. 2016/05/14. 2016;73(20):3861–85. Available from:
  2. Swanson T, Ousey K, Haesler E, Bjarnsholt T, Carville K, Idensohn P, et al. IWII Wound Infection in Clinical Practice consensus document: 2022 update. J Wound Care. 2022;31(Sup12):S10–21. Available from:
  3. van Ramshorst GH, Nieuwenhuizen J, Hop WCJ, Arends P, Boom J, Jeekel J, et al. Abdominal wound dehiscence in adults: development and validation of a risk model. World J Surg. 2010;34(1):20–7. Available from:
  4. Kapoor K, Hassan M. A clinical study of abdominal wound dehiscence with emphasis on surgical management in Bangalore medical college and research institute, Karnataka, India. Int Surg J. 2017; Available from:
  5. Giesen LJX, van den Boom AL, van Rossem CC, den Hoed PT, Wijnhoven BPL. Retrospective Multicenter Study on Risk Factors for Surgical Site Infections after Appendectomy for Acute Appendicitis. Dig Surg. 2016/09/16. 2017;34(2):103–7. Available from:
  6. Sandy-Hodgetts K, Carville K, Leslie GD. Determining risk factors for surgical wound dehiscence: a literature review. Int Wound J. 2013/05/21. 2015;12(3):265–75. Available from:
  7. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev. 2019;99(1):665–706. Available from:
  8. Levin N, Gray T, Farsi M, Dorton D, Miller R. Topical Zinc May Augment Post-Operative Wound Healing, Including Following Mohs Micrographic Surgery: A Review of the Literature. Ski J Cutan Med. 2020;4(5):395–403. Available from:
  9. Lotfi S, Sabiza S, Naddaf H, Rezaie A. Histopathologic Evaluation of Honey-Zinc Oxide Combination on Full-Thickness Experimental Wound Healing in Rats. Iranian Veterinary Journal. 2021;17(3):47–58.
  10. Arslan K, Karahan O, Okus A, Unlu Y, Eryilmaz MA, Ay S, et al. Comparison of topical zinc oxide and silver sulfadiazine in burn wounds: an experimental study. Turkish J Trauma Emerg Surg. 2012;18(5):376–83. Available from:
  11. Dan MM, Sarmah P, Rao Vana D. Wound Healing: Concepts and Updates in Herbal Medicine. International Journal of Medical Research and Health Sciences. 2018;7(1):170–181.
  12. Zhao Y, Dai C, Wang Z, Chen W, Liu J, Zhuo R, et al. A novel curcumin-loaded composite dressing facilitates wound healing due to its natural antioxidant effect. Drug Des Devel Ther. 2019;13:3269–80. Available from:
  13. Mukti MB, Jayawardhita AAG, Arjana AAG. The Effectiveness of Giving Bidara Leaf Extract on Collagen Density in Healing Gingival Incision Wounds in Wistar Rats. Bul Vet Udayana. 2022;419. Available from:
  14. Ågren MS, Phothong N, Burian EA, Mogensen M, Hædersdal M, Jorgensen LN. Topical Zinc Oxide Assessed in Two Human Wound-healing Models. Acta Derm Venereol. 2021;101(5):adv00465–adv00465. Available from:
  15. Bowler P. The role of bacterial communities in wound healing. J Tissue Viability. 2005;15(1):19. Available from:
  16. Lansdown ABG, Mirastschijski U, Stubbs N, Scanlon E, Ågren MS. Zinc in wound healing: Theoretical, experimental, and clinical aspects. Wound Repair Regen. 2007;15(1):2–16. Available from:
  17. Lin P-H, Sermersheim M, Li H, Lee PHU, Steinberg SM, Ma J. Zinc in Wound Healing Modulation. Nutrients. 2017;10(1):16. Available from:
  18. Emiroglu G, Ozergin Coskun Z, Kalkan Y, Celebi Erdivanli O, Tumkaya L, Terzi S, et al. The Effects of Curcumin on Wound Healing in a Rat Model of Nasal Mucosal Trauma. Evid Based Complement Alternat Med. 2017/09/05. 2017;2017:9452392. Available from:
  19. Mohanty C, Das M, Sahoo SK. Sustained Wound Healing Activity of Curcumin Loaded Oleic Acid Based Polymeric Bandage in a Rat Model. Mol Pharm. 2012;9(10):2801–11. Available from:
  20. Kant V, Gopal A, Pathak NN, Kumar P, Tandan SK, Kumar D. Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. Int Immunopharmacol. 2014;20(2):322–30. Available from:
  21. MacKay D, Miller AL. Nutritional Support for Wound Healing, Alternative Medicine Review. 2003;8:369-370.
  22. Muncaster D. The physiology of wound healing and wound assessment. British Journal of Perioperative Nursing. 2001;11(8):362-70
  23. Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem. 2006;290(12):87–96. Available from:
  24. Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R. Curcumin as a wound healing agent. Life Sci. 2014;116(1):1–7. Available from:

How to Cite

Walmiky Budi, M., Hariastawa, A. ., & Matulatan, F. . (2023). Effectiveness of topical turmeric extract compared to topical zinc on fibroblast count and collagen density in clean-contaminated wound: a study on new zealand white rabbit. Bali Medical Journal, 12(1), 647–651.




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