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Formation of woven bone in orthodontic tooth movement tension areas after giving Mangostin by expression of Runx-2 and IL-10

Abstract

Background: Alveolar bone remodeling is very helpful in orthodontic treatment to prevent relapse after treatment is completed with new bone formation. Using natural materials that have a mechanism of action on the periodontal ligament and alveolar bone as a relapse prevention triggers osteogenesis with the formation of new bone in the tension area so that orthodontic treatment can be achieved maximally. This study aims to determine the formation of woven bone in the area attraction of orthodontic tooth movement after administration of Mangostin

Methods: A total of 30 male Wistar rats were divided into 3 groups, namely a negative control group (K1) without treatment, K2 and K3 a positive control group K(+), K2 a positive control group K(+), which was given a mechanical stressor without Mangostin administration and observed for 7 days, K3 positive control group K(+) which was given a mechanical stressor without Mangostin administration and observed for 14 days, K4 and K5 treatment group (P), K4 treatment group (P) where the group was given a mechanical stressor and Mangostin which was observed for 7 days, K5 the treatment group (P) where the group was given a mechanical stressor and Mangostin which was observed for 14 days. Dara was analyzed using SPSS version 23 for Windows.

Results: It was seen that the administration of Mangostin was very effective in increasing the expression of Runx-2, IL-10 and the formation of woven bone in the area of ​​higher tension than without the administration of Mangostin significantly (p<0.05). There was a significant increase (p<0.05) of Runx-2 and IL-10 in the treated group compared to the control group on day 7 and day 14, respectively.

Conclusion: The administration of Mangostin effectively prevented orthodontic relapse by increasing the expression of Runx-2 and Il-10 and accelerating the formation of woven bone in the tension area of ​​orthodontic movement.

References

  1. Lee W, Eo SR, Choi JH, Kim YM, Nam MH, Seo YK. The Osteogenic Differentiation of Human Dental Pulp Stem Cells through G0/G1 Arrest and the p-ERK/Runx-2 Pathway by Sonic Vibration. Int J Mol Sci. 2021;22(18):10167.
  2. Andrade Jr I, Taddei SRA, Souza PEA. Inflammation and Tooth Movement: The Role of Cytokines, Chemokines, and Growth Factor. Seminars in Orthodontics. 2012;18(4):257-269.
  3. Orth M, Shenar AK, Scheuer C, Braun BJ, Herath SC, Holstein JH, et al. VEGF-loaded mineral-coated microparticles improve bone repair and are associated with increased expression of epo and RUNX-2 in murine non-unions. J Orthop Res. 2019;37(4):821-831.
  4. Luo XH, Liao EY, Su X. Progesterone upregulates TGF-b isoforms (b1, b2, and b3) expression in normal human osteoblast-like cells. Calcif Tissue Int. 2002;71(4):329-334.
  5. Prall WC, Haasters F, Heggebö J, Polzer H, Schwarz C, Gassner C, et al. Mesenchymal stem cells from osteoporotic patients feature impaired signal transduction but sustained osteoinduction in response to BMP-2 stimulation. Biochem Biophys Res Commun. 2013;440(4):617-622.
  6. Sharif MO, Waring DT. Contemporary orthodontics: the micro-screw. Br Dent J. 2013;214(8):403-408.
  7. Henneman S, Von den Hoff JW, Maltha JC. Mechanobiology of tooth movement. Eur J Orthod. 2008;30(3):299-306.
  8. Park HJ, Baek KH, Lee HL, Kwon A, Hwang HR, Qadir AS, et al. Hypoxia inducible factor-1α directly induces the expression of receptor activator of nuclear factor-κB ligand in periodontal ligament fibroblasts. Mol Cells. 2011;31(6):573-578.
  9. Nimeri G, Kau CH, Abou-Kheir NS, Corona R. Acceleration of tooth movement during orthodontic treatment--a frontier in orthodontics. Prog Orthod. 2013;14:42.
  10. Sprogar S, Vaupotic T, Cör A, Drevensek M, Drevensek G. The endothelin system mediates bone modeling in the late stage of orthodontic tooth movement in rats. Bone. 2008;43(4):740-747.
  11. Lilley J, Walters BG, Heath DA, Drolc Z. In vivo and in vitro precision for bone density measured by dual-energy X-ray absorption. Osteoporos Int. 1991;1(3):141-146.
  12. Priya V, Jainu M, Mohan SK, Saraswati P, Gopan CS. Antimicrobial activity of pericarp extract of garcinia mangosatan linn. International Journal of Pharma Sciences and Research. 2010;1(8):278-281.
  13. Jiang DJ, Dai Z, Li YJ. Pharmacological effects of xanthones as cardiovascular protective agents. Cardiovasc Drug Rev. 2004;22(2):91-102.
  14. Yodhnu S, Sirikatitham A, Wattanapiromsakul C. Validation of LC for the determination of alpha-mangostin in mangosteen peel extract: a tool for quality assessment of Garcinia mangostana L. J Chromatogr Sci. 2009;47(3):185-189.
  15. Nakatani K, Atsumi M, Arakawa T, Oosawa K, Shimura S, Nakahata N, et al. Inhibitions of histamine release and prostaglandin E2 synthesis by mangosteen, a Thai medicinal plant. Biol Pharm Bull. 2002;25(9):1137-1141.
  16. Akao Y, Nakagawa Y, Nozawa Y. Anti-cancer effects of xanthones from pericarps of mangosteen. Int J Mol Sci. 2008;9(3):355-370.
  17. Nakagawa Y, Iinuma M, Naoe T, Nozawa Y, Akao Y. Characterized mechanism of alpha-mangostin-induced cell death: caspase-independent apoptosis with release of endonuclease-G from mitochondria and increased miR-143 expression in human colorectal cancer DLD-1 cells. Bioorg Med Chem. 2007;15(16):5620-5628.
  18. Jung HA, Su BN, Keller WJ, Mehta RG, Kinghorn AD. Antioxidant xanthones from the pericarp of Garcinia mangostana (Mangosteen). J Agric Food Chem. 2006;54(6):2077-2082.
  19. Liu SH, Lee LT, Hu NY, Huange KK, Shih YC, Munekazu I, et al. Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells. Chin Med. 2012;7(1):19.
  20. Lee JW, Juliano R. Mitogenic signal transduction by integrin- and growth factor receptor-mediated pathways. Mol Cells. 2004;17(2):188-202.
  21. Indharty S, Japardi I, Siahaan AMP, Tandean S. Mangosteen extract reduce apoptosis via inhibition of oxidative process in rat model of traumatic brain injury. Bali Medical Journal. 2019;8(1):227-232.
  22. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, et al. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell. 2002;108(1):17-29.
  23. Tanaka S, Matsuzaka K, Sato D, Inoue T. Characteristics of newly formed bone during guided bone regeneration: analysis of cbfa-1, osteocalcin, and VEGF expression. J Oral Implantol. 2007;33(6):321-326.
  24. Karsenty G. The complexities of skeletal biology. Nature. 2003;423(6937):316-318.
  25. Niikura M, Inoue S, Kobayashi F. Role of interleukin-10 in malaria: focusing on coinfection with lethal and nonlethal murine malaria parasites. J Biomed Biotechnol. 2011;2011:383962.
  26. Rucci N. Molecular biology of bone remodelling. Clin Cases Miner Bone Metab. 2008;5(1):49-56.

How to Cite

Arnawati, I. A., & Sudiana, I. K. (2022). Formation of woven bone in orthodontic tooth movement tension areas after giving Mangostin by expression of Runx-2 and IL-10. Bali Medical Journal, 11(3), 1956–1962. https://doi.org/10.15562/bmj.v11i3.3971

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