Skip to main content Skip to main navigation menu Skip to site footer

A comparative study of full-thickness wound healing in rats using Nile tilapia skin and fresh human amnion

  • Nyssa Claresta Adhya Sastri ,
  • Iswinarno Doso Saputro ,
  • Lobredia Zarasade ,


Abstract

Background: Human amnion contains several growth factors, inflammatory inhibitors, collagen and hyaluronic acid, which are proven to accelerate wound healing. The collection, processing, and storage of data have several limitations, especially in remote areas. Nile Tilapia fish (Oreochromis niloticus) skin contains amino acids, collagen, and tilapia piscidin, which allows it to be used as a xenograft. This study analyzes the effectiveness of tilapia fish skin compared to the fresh human amnion.

Method: Twenty-four rats were divided into three groups. Group, I was a full-thickness wound treated with tilapia skin, group II was treated with fresh human amnion, and group III was a control. On the third day, a punch biopsy was performed. The granulation tissue thickness and collagen density were examined. A wound area measurement and histopathological examination of the entire wound were performed on the seventh day.

Results: The speed of epithelialization in the tilapia group compared to the amnion was not significantly different (p = 0.065). On the third day, histopathologically, the tilapia group formed the same amount of granulation tissue as the amnion group. In the middle section between tilapia, amnion and control with the Kruskal-Wallis test showed no significant difference (p = 0.573). There was no significant difference in collagen density on the third day between the three groups at the edges and in the middle of the wound (p = 0.097 and 0.253). The Mann-Whitney test on the seventh day found that the density of collagen between tilapia and amnion groups was not much different (p = 0.126).

Conclusion: The speed of epithelialization, growth of granulation tissue, and collagen density of wound healing in full-thickness rats were not significantly different between Nile tilapia skin and fresh human amnion. The skin of Nile Tilapia fish can be used for wound care or as a temporary biological dressing until the wound can be permanently closed.

Keywords: biological dressing fresh human amnion Nile tilapia skin

References

  1. Schoukens, G. Bioactive dressings to promote wound healing. In S. Rajendran (Ed.), Advanced Textiles for Wound Care. Sawston: Woodhead Publishing. 2009. p114-152.
  2. Dhivya S, Padma VV, Santhini E. Wound dressings - a review. Biomedicine (Taipei). 2015;5(4):22. DOI:10.7603/s40681-015-0022-9.
  3. Demidova-Rice TN, Hamblin MR, Herman IM. Acute and impaired wound healing: pathophysiology and current methods for drug delivery, part 1: normal and chronic wounds: biology, causes, and approaches to care. Adv Skin Wound Care. 2012;25(7):304-314. doi:10.1097/01.ASW.0000416006.55218.d0.
  4. Mohammadi AA, Seyed Jafari SM, Kiasat M, et al. Effect of fresh human amniotic membrane dressing on graft take in patients with chronic burn wounds compared with conventional methods. Burns. 2013;39(2):349-353. doi:10.1016/j.burns.2012.07.010.
  5. Castellanos G, Bernabé-García Á, Moraleda JM, Nicolás FJ. Amniotic membrane application for the healing of chronic wounds and ulcers. Placenta. 2017;59:146-153. doi:10.1016/j.placenta.2017.04.005.
  6. Sitje TS, Grøndahl EC, Sørensen JA. Clinical innovation: fish-derived wound product for cutaneous wounds. Wounds International. 2018 Dec 10;9(4):44-50.
  7. Lima-Junior, E.M. et al. The use of tilapia skin (Oreochromis niloticus), as an occlusive biological dressing, in the treatment of burn wounds. Revista Brasileira de Queimaduras. 2017;16(1). p10-17. DOI:10.33448/rsd-v10i8.17146.
  8. Magnusson S, Baldursson BT, Kjartansson H, Rolfsson O, Sigurjonsson GF. Regenerative and Antibacterial Properties of Acellular Fish Skin Grafts and Human Amnion/Chorion Membrane: Implications for Tissue Preservation in Combat Casualty Care. Mil Med. 2017;182(S1):383-388. doi:10.7205/MILMED-D-16-00142.
  9. Bodea IM, Dîrlea SA, Mureşan C, Fiţ NI, Beteg FI. Clinical Benefits of Using a Smartphone Application to Assess the Wound Healing Process in a Feline Patient - A Case Report. Top Companion Anim Med. 2021;42:100498. doi:10.1016/j.tcam.2020.100498.
  10. Biagioni RB, Carvalho BV, Manzioni R, Matielo MF, Brochado Neto FC, Sacilotto R. Smartphone application for wound area measurement in clinical practice. J Vasc Surg Cases Innov Tech. 2021;7(2):258-261. Published 2021 Feb 26. doi:10.1016/j.jvscit.2021.02.008.
  11. Gurtner G and Wong V. Wound healing : normal and abnormal. In C. H. Thorne (Ed.), Grabb and Smith's Plastic Surgery. Philadelphia: Lippincot Williams & Wilkins. 2014. p13-19.
  12. Oliveira GG, Gasparino E, Castilha LD, et al. Characterization and Strength Quality of the Oryctolagus cuniculus Leather Compared to Oreochromis niloticus Leather. ScientificWorldJournal. 2022;2022:4561404. Published 2022 Oct 14. doi:10.1155/2022/4561404.
  13. Hu Z, Yang P, Zhou C, Li S, Hong P. Marine Collagen Peptides from the Skin of Nile Tilapia (Oreochromis niloticus): Characterization and Wound Healing Evaluation. Mar Drugs. 2017;15(4):102. Published 2017 Mar 30. doi:10.3390/md15040102.
  14. Elbialy ZI, Atiba A, Abdelnaby A, et al. Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression. BMC Vet Res. 2020;16(1):352. Published 2020 Sep 24. doi:10.1186/s12917-020-02566-2.
  15. Dorsett MW and Wysocki A. Rat Models of Skin Wound Healing. In M. Conn (Ed.), Sourcebook of Models for Biomedical Research. New Jersey: Humana Press. 2008. p631-638.
  16. Kirsner RS, Margolis DJ, Baldursson BT, et al. Fish skin grafts compared to human amnion/chorion membrane allografts: A double-blind, prospective, randomized clinical trial of acute wound healing. Wound Repair Regen. 2020;28(1):75-80. doi:10.1111/wrr.12761.
  17. Alves APNN, Lima Júnior EM, Piccolo NS, et al. Study of tensiometric properties, microbiological and collagen content in nile tilapia skin submitted to different sterilization methods. Cell Tissue Bank. 2018;19(3):373-382. doi:10.1007/s10561-017-9681-y.

How to Cite

Sastri, N. C. A., Saputro, I. D., & Zarasade, L. (2022). A comparative study of full-thickness wound healing in rats using Nile tilapia skin and fresh human amnion. Bali Medical Journal, 11(3), 1945–1952. https://doi.org/10.15562/bmj.v11i3.3927
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
2

Total
2

Share

Search Panel

Nyssa Claresta Adhya Sastri
Google Scholar
Pubmed
BMJ Journal

Iswinarno Doso Saputro
Google Scholar
Pubmed
BMJ Journal

Lobredia Zarasade
Google Scholar
Pubmed
BMJ Journal