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Association between IL-6 rs1800795 and IL-1β rs 16944 gene polymorphisms with clinical severity of COVID-19 patients in Surakarta, Central Java, Indonesia

  • Hendrastutik Apriningsih ,
  • Betty Surtawati ,
  • Reviono ,
  • Tonang Dwi Ardyanto - ,
  • Dewi Pratiwi ,
  • Hafi Nurinasari ,


Abstract

Background: Cytokine plays a pivotal role in the pathogenesis of coronavirus disease 2019 (COVID-19). Cytokine storm is characterized by rapid elevation of an inflammatory circulating cytokine such as interleukin-6 (IL-6) and IL-1. However, according to evidence, genetic variables may affect the development and course of infectious diseases. Multiple genetic polymorphisms, mostly single-nucleotide polymorphisms (SNPs), have been linked to this setting's predisposition to viral infections. This study aimed to determine the frequency distribution of IL-6 SNPs rs1800795 and IL-1β SNPs rs16944 and rs1143627 gene polymorphisms and their association with the clinical severity of COVID-19 patients in Surakarta, Indonesia. This study aims to determine the association between IL-6 rs1800795 and IL-1β rs16944 with COVID-19 clinical severity.

Methods: This study used a cross sectional design conducted at Universitas Sebelas Maret Hospital and centralized isolation of the Donohudan Hajj Dormitory from May to November 2021. A total of 120 COVID-19 patients were divided into 3 groups: asymptomatic, mild-moderate, and severe-critical. The detection of IL-6 SNPs rs1800795 and IL-1β SNPs rs16944 was carried out by quantitative PCR (qPCR) examination, and IL-6 and IL-1β were determined by the ELISA method.

Result: There was no significant association between IL-6 SNPs rs1800795 (p=1.000) and IL-1β SNPs rs16944 (p=0.119) with clinical severity. In IL-1β SNPs rs16944 gene polymorphisms, the GG genotype was more commonly found in the asymptomatic group. AG genotype was commonly found in the symptomatic group (mild to critical). There was a significant association between IL-1β levels and clinical severity (p=0.03), whereas the association between IL-6 levels and clinical severity is not significant (p=0.103).

Conclusion: There was a correlation between IL-1β levels with clinical severity. In IL-1β SNPs rs16944, the GG genotype may act as a protective factor, whereas the AG genotype may act as a factor that increases the clinical severity of COVID-19.

Keywords: covid-19 clinical severity IL-6 and IL-1β gene polymorphisms

References

  1. Falahi S, Zamanian MH, Feizollahi P, Rezaiemanesh A, Salari F, Mahmoudi Z, et al. Evaluation of the relationship between IL-6 gene single nucleotide polymorphisms and the severity of COVID-19 in an Iranian population. Cytokine. 2022/04/19. 2022;154:155889. Available from: https://pubmed.ncbi.nlm.nih.gov/35461173
  2. Azmi NU, Puteri MU, Lukmanto D. Cytokine Storm in COVID-19: An Overview, Mechanism, Treatment Strategies, and Stem Cell Therapy Perspective. Pharm Sci Res. 2020;7(4):1–11. Available from: http://dx.doi.org/10.7454/psr.v7i4.1092
  3. Kerget F, Kerget B. Frequency of Interleukin-6 rs1800795 (-174G/C) and rs1800797 (-597G/A) Polymorphisms in COVID-19 Patients in Turkey Who Develop Macrophage Activation Syndrome. Jpn J Infect Dis. 2021;74(6):543–8. Available from: http://dx.doi.org/10.7883/yoken.jjid.2021.046
  4. Soy M, Keser G, Atagündüz P, Tabak F, Atagündüz I, Kayhan S. Cytokine storm in COVID-19: pathogenesis and overview of anti-inflammatory agents used in treatment. Clin Rheumatol. 2020/05/30. 2020;39(7):2085–94. Available from: https://pubmed.ncbi.nlm.nih.gov/32474885
  5. Adli A, Rahimi M, Khodaie R, Hashemzaei N, Hosseini SM. Role of genetic variants and host polymorphisms on COVID-19: From viral entrance mechanisms to immunological reactions. J Med Virol. 2022/02/08. 2022;94(5):1846–65. Available from: https://pubmed.ncbi.nlm.nih.gov/35076118
  6. Jamilloux Y, Henry T, Belot A, Viel S, Fauter M, El Jammal T, et al. Should we stimulate or suppress immune responses in COVID-19? Cytokine and anti-cytokine interventions. Autoimmun Rev. 2020/05/04. 2020;19(7):102567. Available from: https://pubmed.ncbi.nlm.nih.gov/32376392
  7. Batur LK, Hekim N. Correlations of IL-6, IL-6R, IL-10 and IL-17 gene polymorphisms with the prevalence of COVID-2019 infection and its mortality rate  [Internet]. Research Square Platform LLC; 2020. Available from: http://dx.doi.org/10.21203/rs.3.rs-82662/v1
  8. Ye Q, Wang B, Mao J. The pathogenesis and treatment of the `Cytokine Storm' in COVID-19. J Infect. 2020/04/10. 2020;80(6):607–13. Available from: https://pubmed.ncbi.nlm.nih.gov/32283152
  9. Chen T, Lin Y-X, Zha Y, Sun Y, Tian J, Yang Z, et al. A Low-Producing Haplotype of Interleukin-6 Disrupting CTCF Binding Is Protective against Severe COVID-19. MBio. 2021/10/12. 2021;12(5):e0137221–e0137221. Available from: https://pubmed.ncbi.nlm.nih.gov/34634929
  10. Rahimlou B, Ghaffarpour S, Zamani MS, Naghizadeh MM, Ghazanfari T. Association between interleukin-6-174G/C single nucleotide polymorphism with the COVID-19 severity. Daneshvar Med. 2022;30(3):10–22. Available from: http://daneshvarmed.shahed.ac.ir/article_3830.html
  11. García-Ramírez RA, Ramírez-Venegas A, Quintana-Carrillo R, Camarena ÁE, Falfán-Valencia R, Mejía-Aranguré JM. TNF, IL6, and IL1B Polymorphisms Are Associated with Severe Influenza A (H1N1) Virus Infection in the Mexican Population. PLoS One. 2015;10(12):e0144832–e0144832. Available from: https://pubmed.ncbi.nlm.nih.gov/26657940
  12. Keshavarz M, Namdari H, Farahmand M, Mehrbod P, Mokhtari-Azad T, Rezaei F. Association of polymorphisms in inflammatory cytokines encoding genes with severe cases of influenza A/H1N1 and B in an Iranian population. Virol J. 2019;16(1):79. Available from: https://pubmed.ncbi.nlm.nih.gov/31196204
  13. Rogo LD, Rezaei F, Marashi SM, Yekaninejad MS, Naseri M, Ghavami N, et al. Seasonal influenza A/H3N2 virus infection and IL-1Β, IL-10, IL-17, and IL-28 polymorphisms in Iranian population. J Med Virol. 2016;88(12):2078–84. Available from: http://dx.doi.org/10.1002/jmv.24572
  14. Tanimine N, Takei D, Tsukiyama N, Yoshinaka H, Takemoto Y, Tanaka Y, et al. Identification of Aggravation-Predicting Gene Polymorphisms in Coronavirus Disease 2019 Patients Using a Candidate Gene Approach Associated With Multiple Phase Pathogenesis: A Study in a Japanese City of 1 Million People. Crit care Explor. 2021;3(11):e0576–e0576. Available from: https://pubmed.ncbi.nlm.nih.gov/34765983
  15. Kahan SM, Wherry EJ, Zajac AJ. T cell exhaustion during persistent viral infections. Virology. 2015/01/22. 2015;479–480:180–93. Available from: https://pubmed.ncbi.nlm.nih.gov/25620767
  16. Mishra KP, Singh M, Saraswat D, Ganju L, Varshney R. Dysfunctional State of T Cells or Exhaustion During Chronic Viral Infections and COVID-19: A Review. Viral Immunol. 2022;35(4):284–90. Available from: http://dx.doi.org/10.1089/vim.2022.0002
  17. Gupta R, Misra A. COVID19 in South Asians/Asian Indians: Heterogeneity of data and implications for pathophysiology and research. Diabetes Res Clin Pract. 2020/06/10. 2020;165:108267. Available from: https://pubmed.ncbi.nlm.nih.gov/32533988
  18. Makaremi S, Asgarzadeh A, Kianfar H, Mohammadnia A, Asghariazar V, Safarzadeh E. The role of IL-1 family of cytokines and receptors in pathogenesis of COVID-19. Inflamm Res. 2022/06/25. 2022;71(7–8):923–47. Available from: https://pubmed.ncbi.nlm.nih.gov/35751653

How to Cite

Hendrastutik Apriningsih, -, B. S., Reviono, -, T. D. A., Dewi Pratiwi, & Hafi Nurinasari. (2023). Association between IL-6 rs1800795 and IL-1β rs 16944 gene polymorphisms with clinical severity of COVID-19 patients in Surakarta, Central Java, Indonesia. Bali Medical Journal, 12(1), 904–908. https://doi.org/10.15562/bmj.v12i1.3919
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Hendrastutik Apriningsih
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Betty Surtawati
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Reviono
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Tonang Dwi Ardyanto -
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Dewi Pratiwi
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