Background: The employees' understanding of the organization in fact forms organizational climate. One of the most common internal factors affecting the organizational climate is the employees' personality characteristics. Therefore, the aim of this study is to determine the relationship between organizational climate and the personality characteristics of the Fatemeh Alzahra hospital's employees located in Mehriz, Yazd (Iran). Methods: This study is descriptive and correlational carried out cross sectionally. The research population is the various sectors' staff of the teaching hospital in Mehriz that 140 of them were selected randomly. In order to collect data, the two organizational climate questionnaires of Sam Deep and Lyle Sussman and Eysenck Personality Questionnaire were used. Data using Spss16 software and T-test and Pearson correlation tests were analyzed. Results: According to our findings, there is no relation between the personality traits of employees and organizational climate. As well as among each of the personality dimensions and organizational climate there are no relationships. Only observed relationship among the employees' personality characteristics and one of the dimensions of organizational climate was as the organizational climate based on agreement on procedures (P<0.01). Conclusion: The findings show that there is a significant relationship between organizational climate based on agreement on procedures and the employees' personality characteristics of the hospital, Therefore, it is recommended that managers and planners by providing required and healthy human force in terms of personality type and determining the procedures appropriate and targeted for the personnel working in this hospital, help the Organization achieve its excellent goals.