Number of e-cadherin and VEGF in WiDr cell line post-induced by extract ethanol Annona muricata leaf

Yoni Astuti; Agus Suharto; Sabtanti Harimurti; Helmi Hana Prinanda; Wahyu Joko Priambodo

doi:10.15562/bmj.v12i1.3736

Number of e-cadherin and VEGF in WiDr cell line post-induced by extract ethanol Annona muricata leaf

Yoni Astuti ,

Agus Suharto ,

Sabtanti Harimurti ,

Helmi Hana Prinanda ,

Wahyu Joko Priambodo ,

pdf |
DOI: https://doi.org/10.15562/bmj.v12i1.3736 |
Published: 2023-02-02

Abstract

Introduction: Soursop leaves have benefits including anticancer, antifungal, and antibacterial. Soursop leaves are proven to contain various phytochemicals, namely alkaloids (ALKs), essential oils and acetogenins. Annonaceous acetogenins compounds soursop cytotoxic effect on cancer cells. One of the reasons for the malignancy of cancer cells is their ability to move to other tissues. Migration ability was related with the expression of e-cadherin, in cancer cells the expression of e-cadherin was low. Malignancy is also related to the amount of VEGF, in the type of tumor VEGF expression increases. Therefore, the aim of this study was to measure the amount of e-cadherin and VEGF of the WiDr colon cancer cell model after being induced by soursop leaf ethanol extract.

Methods: Ethanol extract of soursop leaves is obtained by maceration. The measurement of the amount of e-cadherin used the elisa kit e-cadherin while the amount of VEGF was used by the elisa kit VEGF. The amount of e-cadherin and VEGF measurement used was an elisa reader at a wavelength of 560 NM.

Results: The results showed there were significant differences between various doses of extract ethanol Annona muricata to the number of e-cadherin. There were significant differences between various doses of Extract ethanol Annona muricata to the number of VEGF.

Conclusions: Ethanol extract Annona muricata leaf showed the anti migration and anti angiogenesis effect on WiDr cell line.

References

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Ilango S, Sahoo DK, Paital B, Kathirvel K, Hati AK, Behera TR, et al. A Review on Annona muricata and Its Anticancer Activity. 2022;1–31.
Muñoz-Gómez RJ, Rivero-Cruz I, Ovalle-Magallanes B, Linares E, Bye R, Tovar AR, et al. Antidiabetic Sterols from Peniocereus greggii Roots. ACS omega. 2022;7(15):13144–54.
Thomas S, Paul J, Shrungeswara A, Biswas S, Shah A, Subraya C, et al. Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats. Asian Pac J Trop Med. 2019;12(6):272–82.
Falodun A, Osakue J, Uzoekwe AS, Qiu S-X. Phytochemical and Anticancer Studies on Ten Medicinal Plants Used in Nigeria. Bayero J Pure Appl Sci. 2011;4.
Hasmila I, Natsir H, Soekamto NH. Phytochemical analysis and antioxidant activity of soursop leaf extract (Annona muricata Linn.). J Phys Conf Ser. 2019;1341(3).
Widyastuti D, Rahayu P. Antioxidant Capacity Comparison of Ethanolic Extract of Soursop (Annona muricata Linn.) Leaves and Seeds as Cancer Prevention Candidate. Biol Med Nat Prod Chem. 2017;6:1.
George ML, Tutton MG, Janssen F, Arnaoutz A, Abulafi AM, Eccles SA, et al. VEGF-A, VEGF-C, and VEGF-D in colorectal cancer progression. Neoplasia. 2001;3(5):420–7.
Sampedro MF, Izaguirre MF, Sigot V, Muller M. expression pattern during zebrafish embryonic epidermis development [ version 3 ; referees : 3 approved ] Referee Status : 2019;1–21.
Buda A, Pignatelli M. E-cadherin and the cytoskeletal network in colorectal cancer development and metastasis. Cell Commun Adhes. 2011;18(6):133–43.
Jung YD, Mansfield PF, Akagi M, Takeda A, Liu W, Bucana CD, et al. Effects of combination anti-vascular endothelial growth factor receptor and anti-epidermal growth factor receptor therapies on the growth of gastric cancer in a nude mouse model. Eur J Cancer. 2002;38(8):1133–40.
Yang X, Zhang Y, Hosaka K, Andersson P, Wang J, Tholander F, et al. VEGF-B promotes cancer metastasis through a VEGF-A-independent mechanism and serves as a marker of poor prognosis for cancer patients. Proc Natl Acad Sci U S A. 2015;112(22):E2900–9.
Panigoro SS, Karsono R, Sari L. E-cadherin and Vimentin as Predictors of Resistance to Preoperative Systemic Therapy in Patients with Advanced Breast Cancer. eJournal Kedokt Indones. 2017;4(3):27–30.
Shamir ER, Pappalardo E, Jorgens DM, Coutinho K, Tsai WT, Aziz K, et al. Twist1-induced dissemination preserves epithelial identity and requires E-cadherin. J Cell Biol. 2014;204(5):839–56.
Zhao XH, Wang ZR, Chen CL, Di L, Bi ZF, Li ZH, et al. Molecular detection of epithelial-mesenchymal transition markers in circulating tumor cells from pancreatic cancer patients: Potential role in clinical practice. World J Gastroenterol. 2019;25(1):138–50.
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Miura K, Fujibuchi W, Sasaki I. Alternative pre-mRNA splicing in digestive tract malignancy. Cancer Sci. 2011;102(2):309–16.
Astuti Y, Priambodo W, Harimurti S, Suharto A. Apoptosis Effect Of Ethanol Extract of Soursoup Leaves on WiDr Cancer cell Line. Int Conf Sustain Innov (ICoSI). 2021;(August 2021):25–6.
Liu X, Chu K-M. E-Cadherin and Gastric Cancer: Cause, Consequence, and Applications. De Re V, editor. Biomed Res Int. 2014;2014:637308. Available from: https://doi.org/10.1155/2014/637308
Santarosa M, Maestro R. The autophagic route of e-cadherin and cell adhesion molecules in cancer progression. Cancers (Basel). 2021;13(24).
Siemann DW, Norris CM, Ryan A, Shi W. Impact of tumor cell VEGF expression on the in vivo efficacy of vandetanib (ZACTIMA; ZD6474). Anticancer Res. 2009;29(6):1987–92.
Bhattacharya R, Fan F, Wang R, Ye X, Xia L, Boulbes D, et al. Intracrine VEGF signalling mediates colorectal cancer cell migration and invasion. Br J Cancer. 2017;117(6):848–55. Available from: http://dx.doi.org/10.1038/bjc.2017.238
Duffy A, Bouchier-Hayes, DJ Harmey J. Vascular Endothelial Growth Factor (VEGF) and Its Role in Non-Endothelial Cells: Autocrine Signalling by VEGF. 2013;
Al-Shammari A, Razzaq B, Rasheed A, Falah I. Anti-Angiogenic Activity Of Annona Reticulata N-Hexane Seeds Extract : In Vivo Study. Biochem Cell Arch. 2021;21:4327–35.
Yoni Astuti, Wahyu Joko Priambodo,Rahmah, Dara Ayu Septiyana. Overview of wound assay methods extract ethanol leaves soursop (annona muricata) on widr colon cancer cells. Bali MedJ. 2021; 10 (3); Special Issue ICONURS: 1385-1389 .Avaiable from : https://doi.org/10.15562/bmj.v10i3.2979.
Hassan I, Mshimesh, B. A.Al-Shammari A, Rasheed A. Anti-Angiogenic Activity of Annona Reticulata N-Hexane Seeds Extract: in Vivo Study. Biochem Cell Arch. 2021;21(2):4327–4335.
Niu J, Sun Y, Guo Q, Niu D, Liu B. miR-1 inhibits cell growth, migration, and invasion by targeting VEGFA in osteosarcoma cells. Dis Markers. 2016;2016. Available from: https://link.gale.com/apps/doc/A508841459/HRCA?u=anon~62c6daf3&sid=googleScholar&xid=9be67cd0
Astekar M, Joshi A, Ramesh G, Metgud R. Expression of vascular endothelial growth factor and microvessel density in oral tumorigenesis. J Oral Maxillofac Pathol. 2012;16(1):22–6.

[1] WHO. Cancer. 2022.

[2] Schreuders EH, Ruco A, Rabeneck L, Schoen RE, Sung JJY, Young GP, et al. Colorectal cancer screening: A global overview of existing programmes. Gut. 2015;64(10):1637–49.

[3] Ilango S, Sahoo DK, Paital B, Kathirvel K, Hati AK, Behera TR, et al. A Review on Annona muricata and Its Anticancer Activity. 2022;1–31.

[4] Muñoz-Gómez RJ, Rivero-Cruz I, Ovalle-Magallanes B, Linares E, Bye R, Tovar AR, et al. Antidiabetic Sterols from Peniocereus greggii Roots. ACS omega. 2022;7(15):13144–54.

[5] Thomas S, Paul J, Shrungeswara A, Biswas S, Shah A, Subraya C, et al. Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats. Asian Pac J Trop Med. 2019;12(6):272–82.

[6] Falodun A, Osakue J, Uzoekwe AS, Qiu S-X. Phytochemical and Anticancer Studies on Ten Medicinal Plants Used in Nigeria. Bayero J Pure Appl Sci. 2011;4.

[7] Hasmila I, Natsir H, Soekamto NH. Phytochemical analysis and antioxidant activity of soursop leaf extract (Annona muricata Linn.). J Phys Conf Ser. 2019;1341(3).

[8] Widyastuti D, Rahayu P. Antioxidant Capacity Comparison of Ethanolic Extract of Soursop (Annona muricata Linn.) Leaves and Seeds as Cancer Prevention Candidate. Biol Med Nat Prod Chem. 2017;6:1.

[9] George ML, Tutton MG, Janssen F, Arnaoutz A, Abulafi AM, Eccles SA, et al. VEGF-A, VEGF-C, and VEGF-D in colorectal cancer progression. Neoplasia. 2001;3(5):420–7.

[10] Sampedro MF, Izaguirre MF, Sigot V, Muller M. expression pattern during zebrafish embryonic epidermis development [ version 3 ; referees : 3 approved ] Referee Status : 2019;1–21.

[11] Buda A, Pignatelli M. E-cadherin and the cytoskeletal network in colorectal cancer development and metastasis. Cell Commun Adhes. 2011;18(6):133–43.

[12] Jung YD, Mansfield PF, Akagi M, Takeda A, Liu W, Bucana CD, et al. Effects of combination anti-vascular endothelial growth factor receptor and anti-epidermal growth factor receptor therapies on the growth of gastric cancer in a nude mouse model. Eur J Cancer. 2002;38(8):1133–40.

[13] Yang X, Zhang Y, Hosaka K, Andersson P, Wang J, Tholander F, et al. VEGF-B promotes cancer metastasis through a VEGF-A-independent mechanism and serves as a marker of poor prognosis for cancer patients. Proc Natl Acad Sci U S A. 2015;112(22):E2900–9.

[14] Panigoro SS, Karsono R, Sari L. E-cadherin and Vimentin as Predictors of Resistance to Preoperative Systemic Therapy in Patients with Advanced Breast Cancer. eJournal Kedokt Indones. 2017;4(3):27–30.

[15] Shamir ER, Pappalardo E, Jorgens DM, Coutinho K, Tsai WT, Aziz K, et al. Twist1-induced dissemination preserves epithelial identity and requires E-cadherin. J Cell Biol. 2014;204(5):839–56.

[16] Zhao XH, Wang ZR, Chen CL, Di L, Bi ZF, Li ZH, et al. Molecular detection of epithelial-mesenchymal transition markers in circulating tumor cells from pancreatic cancer patients: Potential role in clinical practice. World J Gastroenterol. 2019;25(1):138–50.

[17] Wurdeman SR, Stevens PM, Campbell JH, Davie-Smith F, Coulter E, Kennon B, et al. 乳鼠心肌提取 HHS Public Access. Physiol Behav. 2017;176(5):498–503. Available from: https://journals.lww.com/00006479-201842050-00005%0Ahttp://files/16/Wurdeman et al. - 2018 - Mobility Analysis of AmpuTees (MAAT I) Quality of.pdf

[18] Kong S-C. Parents’ perceptions of e-learning in school education: implications for the partnership between schools and parents. Technol Pedagog Educ. 2018;27(1):15–31. Available from: https://doi.org/10.1080/1475939X.2017.1317659.

[19] Efstathiou JA, Liu D, Wheeler JMD, Kim HC, Beck Ne, Ilyas M, Karayiannaki AJ, Mortensen NJMCC, Kmiot W, Playford RJ, Pignatelli M, And Bodmer WF. Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. Proc. Natl. Acad. Sci. 1999; 96: 2316–2321.

[20] Miura K, Fujibuchi W, Sasaki I. Alternative pre-mRNA splicing in digestive tract malignancy. Cancer Sci. 2011;102(2):309–16.

[21] Astuti Y, Priambodo W, Harimurti S, Suharto A. Apoptosis Effect Of Ethanol Extract of Soursoup Leaves on WiDr Cancer cell Line. Int Conf Sustain Innov (ICoSI). 2021;(August 2021):25–6.

[22] Liu X, Chu K-M. E-Cadherin and Gastric Cancer: Cause, Consequence, and Applications. De Re V, editor. Biomed Res Int. 2014;2014:637308. Available from: https://doi.org/10.1155/2014/637308

[23] Santarosa M, Maestro R. The autophagic route of e-cadherin and cell adhesion molecules in cancer progression. Cancers (Basel). 2021;13(24).

[24] Siemann DW, Norris CM, Ryan A, Shi W. Impact of tumor cell VEGF expression on the in vivo efficacy of vandetanib (ZACTIMA; ZD6474). Anticancer Res. 2009;29(6):1987–92.

[25] Bhattacharya R, Fan F, Wang R, Ye X, Xia L, Boulbes D, et al. Intracrine VEGF signalling mediates colorectal cancer cell migration and invasion. Br J Cancer. 2017;117(6):848–55. Available from: http://dx.doi.org/10.1038/bjc.2017.238

[26] Duffy A, Bouchier-Hayes, DJ Harmey J. Vascular Endothelial Growth Factor (VEGF) and Its Role in Non-Endothelial Cells: Autocrine Signalling by VEGF. 2013;

[27] Al-Shammari A, Razzaq B, Rasheed A, Falah I. Anti-Angiogenic Activity Of Annona Reticulata N-Hexane Seeds Extract : In Vivo Study. Biochem Cell Arch. 2021;21:4327–35.

[28] Yoni Astuti, Wahyu Joko Priambodo,Rahmah, Dara Ayu Septiyana. Overview of wound assay methods extract ethanol leaves soursop (annona muricata) on widr colon cancer cells. Bali MedJ. 2021; 10 (3); Special Issue ICONURS: 1385-1389 .Avaiable from : https://doi.org/10.15562/bmj.v10i3.2979.

[29] Hassan I, Mshimesh, B. A.Al-Shammari A, Rasheed A. Anti-Angiogenic Activity of Annona Reticulata N-Hexane Seeds Extract: in Vivo Study. Biochem Cell Arch. 2021;21(2):4327–4335.

[30] Niu J, Sun Y, Guo Q, Niu D, Liu B. miR-1 inhibits cell growth, migration, and invasion by targeting VEGFA in osteosarcoma cells. Dis Markers. 2016;2016. Available from: https://link.gale.com/apps/doc/A508841459/HRCA?u=anon~62c6daf3&sid=googleScholar&xid=9be67cd0

[31] Astekar M, Joshi A, Ramesh G, Metgud R. Expression of vascular endothelial growth factor and microvessel density in oral tumorigenesis. J Oral Maxillofac Pathol. 2012;16(1):22–6.

Number of e-cadherin and VEGF in WiDr cell line post-induced by extract ethanol Annona muricata leaf

Abstract

References

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