Skip to main content Skip to main navigation menu Skip to site footer

Expression of Superoxide Dismutase-1 (SOD-1) and changes of corneal endothelial morphology after phacoemulsification with hypothermic perfusion

  • Afia Nuzila Fadhlina ,
  • Luki Indriaswati ,
  • Nurwasis ,
  • Dyah Fauziah ,
  • Mahmudah ,


Abstract

Background: Hypothermic perfusion can be used as adjunctive therapy to reduce corneal endothelial cell damage during phacoemulsification procedures. This study aims to evaluate the effect of hypothermic perfusion on the expression of Superoxide Dismutase-1 (SOD1) and morphological changes in the corneal endothelium after ultrasound energy of phacoemulsification exposure.

Methods: Sixteen New Zealand white rabbits (n=16 eyes) were randomly divided into two groups and exposed to ultrasound energy of phacoemulsification. The control group was administered room temperature (RT) (24°C) Balanced Salt Solution (BSS) intraocular perfusion, and the treatment group was administered hypothermic (4°C) BSS intraocular perfusion. Coefficient of Variation (CV) and hexagonality were measured before and one day after surgery with specular microscopy. The expression of SOD1 was examined by immunohistochemistry staining. Data were analyzed using SPSS version 26 for Windows.

Results: The result showed that the SOD1 expression was significantly lower in the hypothermic perfusion group compared with the control group (p=0.024, p<0.05). There were no significant differences between the two groups in CV changes (p=0.494, p>0.05) and hexagonality changes (p=0.916, p>0.05). There was no correlation between the expression of SOD1 and change in CV (p=0.188, p>0.05) or the expression of SOD1 and change in hexagonality (p=0.763, p>0.05).

Conclusion: Hypothermic perfusion suppresses all metabolic processes, including the expression of SOD1 and ROS production. The coefficient of variation and hexagonality as a representative of corneal endothelial morphology changes also decrease with hypothermic perfusion after ultrasound exposure but are not statistically significant. There is no correlation between the expression of SOD1 and changes in CV and hexagonality.

Keywords: Corneal Endothelial CV Hypothermic Perfusion Phacoemulsification SOD

References

  1. Igarashi T, Ohsawa I, Kobayashi M, Umemoto Y, Arima T, Suzuki H, et al. Effects of Hydrogen in Prevention of Corneal Endothelial Damage During Phacoemulsification: A Prospective Randomized Clinical Trial. Am J Ophthalmol. 2019;207:10–17.
  2. Park J, Yum HR, Kim MS, Harrison AR, Kim EC. Comparison of phaco-chop, divide-and-conquer, and stop-and-chop phaco techniques in microincision coaxial cataract surgery. J Cataract Refract Surg. 2013;39(10):1463–1469.
  3. Stefan P, Sanziana I, Liliana V, Costin L, Vanessa P, Radu C. Pseudophakic bullous keratopathy. Rom J Ophthalmol. 2017;61(2):90–94.
  4. Wan W, Jiang L, Ji Y, Xun Y, Xiong L, Xiang Y, et al. Effect of hypothermic perfusion on phacoemulsification in eyes with hard nuclear cataract: randomized trial. J Cataract Refract Surg. 2019;45(12):1717–1724.
  5. Al-Sharkawy H. Comparison between effects of Ringer′s and Ringer′s lactate irrigating solutions on corneal endothelium during phacoemulsfication. J Egypt Ophthalmol Soc. 2015;108(2):67.
  6. Takahashi H. Corneal endothelium and phacoemulsification. Cornea. 2016;35(11):S3–7.
  7. Álvarez-Barrios A, Álvarez L, García M, Artime E, Pereiro R, González-Iglesias H. Antioxidant defenses in the human eye: A focus on metallothioneins. Antioxidants. 2021;10(1):89.
  8. Vallabh NA, Romano V, Willoughby CE. Mitochondrial dysfunction and oxidative stress in corneal disease. Mitochondrion. 2017;36:103–113.
  9. Chaurasia S, Vanathi M. Specular microscopy in clinical practice. Indian J Ophthalmol [Internet]. 2021;69(3):517-524.
  10. Jiang L, Wan W, Xun Y, Xiong L, Wu B, Xiang Y, et al. Effect of hypothermic perfusion on phacoemulsification in cataract patients complicated with uveitis: A randomised trial. BMC Ophthalmol. 2020;20(1):232.
  11. Fidianto A, Komaratih E, Sasono W, Sandhika W, Notobroto HB. Intracameral injection of limbal mesenchymal stem cells secretome alleviate inflammation with delayed structural recovery on corneal endothelial cells in phacoemulsified rabbit eyes. Biochem Cell Arch. 2019;19:4729–4736.
  12. Widayanto E, Nurwasis, Hermawan D, Fidianto A, Putri P, Rindiastuti Y, et al. Intracameral injection of limbal mesenchymal stem cells conditioned media (LMSCs-CM) improve clinical outcome with delayed on Na-K ATPase corneal endothelial pump recovery in phacoemulsified rabbit eyes. Biochem Cell Arch. 2019;19:4889–4897.
  13. Meduri A, Aragona P, Testagrossa B, Scolaro S, Gurgone S, Bonanno L, et al. An alternative approach to cataract surgery using BSS temperature of 2.7°C. Appl Sci. 2020;10(8):1-10.
  14. Nemet AY, Assia EI, Meyerstein D, Meyerstein N, Gedanken A, Topaz M. Protective effect of free-radical scavengers on corneal endothelial damage in phacoemulsification. J Cataract Refract Surg. 2007;33(2):310–315.
  15. Younus H. Younus H. Therapeutic potentials of superoxide dismutase. Int J Health Sci (Qassim). 2018;12(3):88–93.
  16. Behndig A, Brannstrom T, Johansson B. Superoxide dismutase isoenzymes in the normal and diseased human cornea. Investig Ophthalmol Vis Sci. 2001;42(10):2293–2296.
  17. Čejková J, Vejražka M, Pláteník J, Štípek S. Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea. Exp Gerontol. 2004;39(10):1537–1543.
  18. Uçakhan ÖÖ, Karel F, Kanpolat A, Devrim E, Durak I. Superoxide dismutase activity in the lens capsule of patients with pseudoexfoliation syndrome and cataract. J Cataract Refract Surg. 2006;32(4):618–622.
  19. Donma O, Yorulmaz EÖ, Pekel H, Suyugül N. Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patients. Curr Eye Res. 2002;25(1):9–16.
  20. Özmen B, Özmen D, Erkin E, Güner I, Habif S, Baymdir O. Lens superoxide dismutase and catalase activities in diabetic cataract. Clin Biochem. 2002;35(1):69–72.
  21. Wang X, Sun J, Dang GF, Gao Y, Duan L, Wu XY. Antioxidant content and cytological examination of aqueous fluid from patients with age-related cataracts at different stages. Genet Mol Res. 2015;14(2):6251–6255.
  22. Nurwasis, Suhendro G, Sudiana IK. The role of SOD, catalase, HSP-27, HSP-70, and TNF-α expression in apoptosis of retinal ganglion cells after intra ocular pressure increase on rattus norvegicus. Indian J Public Heal Res Dev. 2019;10(7):1174–1178.
  23. Wang Z, Cai F, Chen X, Luo M, Hu L, Lu Y. The Role of Mitochondria-Derived Reactive Oxygen Species in Hyperthermia-Induced Platelet Apoptosis. PLoS One. 2013;8(9):1–14.
  24. Hendriks KDW, Brüggenwirth IMA, Maassen H, Gerding A, Bakker B, Porte RJ, et al. Renal temperature reduction progressively favors mitochondrial ROS production over respiration in hypothermic kidney preservation. J Transl Med. 2019;17(1):1–10.
  25. Tang Y, Chen X, Zhang X, Tang Q, Liu S, Yao K. Clinical evaluation of corneal changes after phacoemulsification in diabetic and non-diabetic cataract patients, a systematic review and meta-analysis. Sci Rep [Internet]. 2017;7(1):1–17.
  26. Pardasani R, Lohiya S. Study of Changes in Corneal Thickness and Corneal Endothelial Cell Density after Phacoemulsification Cataract Surgery. J Evol Med Dent Sci. 2021;10(12):866–872.
  27. Praveen MR, Vasavada AR, Shah R, Vasavada VA. Effect of room temperature and cooled intraocular irrigating solution on the cornea and anterior segment inflammation after phacoemulsification: A randomized clinical trial. Eye. 2009;23(5):1158–1163.
  28. Budiman B. Comparison of Endothelial Cell Density, Morphological Changes and Central Corneal Thickness after Phacoemulsification between Diabetic and Non-Diabetic Patients. Open Ophthalmol J. 2020;14(1):15–20.
  29. Ataş M, Demircan S, Karatepe Haşhaş AS, Gülhan A, Zararsız G. Comparison of corneal endothelial changes following phacoemulsification with transversal and torsional phacoemulsification machines. Int J Ophthalmol. 2014;7(5):822–827.
  30. Kim DH, Wee WR, Hyon JY. The pattern of early corneal endothelial cell recovery following cataract surgery: cellular migration or enlargement? Graefe's Arch Clin Exp Ophthalmol. 2015;253(12):2211–2216.
  31. Anom IGNS, Jayanegara WG, Sugiana IGNM, Raka IGW. Phacoemulsification and sutureless large-incision manualcataract extraction change corneal sensibility. Bali Medical Journal. 2013;2(3):108-112.

How to Cite

Fadhlina, A. N., Indriaswati, L., Nurwasis, Fauziah, D., & Mahmudah. (2022). Expression of Superoxide Dismutase-1 (SOD-1) and changes of corneal endothelial morphology after phacoemulsification with hypothermic perfusion. Bali Medical Journal, 11(3), 1521–1526. https://doi.org/10.15562/bmj.v11i3.3734
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
0

Total
0

Share