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Antibacterial activity of Sentul fruit peel extract (Sandoricum koetjape) against Streptococcus mutans and Staphylococcus aureus

Abstract

Introduction: Infectious mouth diseases are caused by microorganisms such as      Staphylococcus aureus and Streptococcus mutans. Sentul fruit peel extract contains several phytochemical compounds, flavonoid compounds, saponins and tannins are aromatic hydroxyl groups that act as antibacterial so that they can be used as a treatment for infections of the mouth. This study aimed to determine the inhibitory ability of Sentul fruit peel extract (Sandoricum koetjape) against Streptococcus mutans and Staphylococcus Aureus bacteria which can cause oral infections.

Method: Sentul fruit peel simplicia was extracted by maceration method with ethanol for 24 hours. The maceration method was chosen in this study because it is a method that is easy to do and uses simple tools, which is enough to soak the sample in a solvent. A filtering process followed this and the filtrate was then evaporated with a vacuum rotary evaporator at a temperature of 45oC, so that a thick extract was produced. The maceration process was repeated 2 times. After the extraction process, then proceed with liquid-liquid fractionation using distilled water. The thick extract was put into a separating funnel and distilled water was added. Then it was shaken and the aquadest fraction was taken, followed by evaporation with a vacuum rotary evaporator at 45oC, so that the aqua fraction of the ethanol extract was produced.

Result: The results showed that the Sentul fruit peel extract was able to inhibit the growth of      S. mutans and S. aureus bacteria as seen from the formation of a clear zone. Based on the ANOVA test, the extract concentration treatment had a significant effect (p<0.005) on the diameter of the clear zone on S. mutans and S. aureus bacteria. Positive control (streptomycin) showed significant differences in Duncan's test, because it produced the greatest antibacterial activity against test bacteria compared to negative control and various extract concentrations. The diameter of the clear zone in the positive control against S. mutans and S. aureus bacteria were 17.63±0.28 mm and 17.62±1.04 mm, respectively. Sentul peel ethanol extract of aqua fraction at a concentration of 100% gave the highest inhibition zone compared to concentrations of 75%, 50%, and 25%, although it was still smaller than the diameter of the clear zone in the positive control. In bacteria S. mutans, the ethanol extract of Sentul peel aqua fraction at a concentration of 100% gave a clear zone diameter of 14.31±1.06 mm and in S. aureus bacteria it gave a clear zone diameter of 15.34±1.81 mm.

Conclusion: Sentul fruit peel extract (Sandoricum koetjape) has the ability to inhibit Streptococcus mutans bacteria and Staphylococcus Aureus bacteria.

References

  1. Ambarwati. Study On Potential Actinomycetes To Produce
  2. Antibiotik Dari Rhizosfer Antibiotic Taken From
  3. Mimosa Pudica L. Science and Technology Journal. 2007;8(1):1-14.
  4. Murray PR, Rosenthal KS, Pfaller MA. Medical microbiology. Philadelphia: Elsevier/Saunders; 2013.
  5. Sakagami Y, Iinuma M, Piyasena KG, Dharmaratne HR. Antibacterial activity of alpha-mangostin against vancomycin resistant Enterococci (VRE) and synergism with antibiotics. Phytomedicine. 2005;12(3):203-8. doi: 10.1016/j.phymed.2003.09.012.
  6. Tanjaya T, Elza I. IL-1ß Genetic Polimorphism in Menopause Women as Periodontal Disease Risk Factor. Jurnal Dental Indonesia. 2011;18(1):1-5.
  7. Krismariono A. Basic Principles of Scaling and Root Planing in Periodontal Treatment. Periodontic Journal. 2009;1(1):1-5
  8. Hoowink B. Preventive Dentistry. Yogyakarta: Gajah Mada University Press, 1993: 275-76
  9. Rasadah MA, Khozirah S, Aznie AA, Nik MM. Anti-inflammatory agents from Sandoricum koetjape Merr. Phytomedicine. 2004;11(2-3):261-3. doi: 10.1078/0944-7113-00339.
  10. Bumi MB, Heliawaty L, Hermawati E, Syah YM. Four limonoids from the seeds extract of Sandoricum koetjape. J Nat Med. 2019 Jun;73(3):641-647. doi: 10.1007/s11418-019-01303-w.
  11. Ismail IS, Ito H, Hatano T, Taniguchi S, Yoshida T. Modified limonoids from the leaves of Sandoricum koetjape. Phytochemistry. 2003;64(8):1345-9. doi: 10.1016/s0031-9422(03)00500-4.
  12. Wirata I, Agung AA, Arini N, Nuratni N. Sentul Fruit (Sandoricum koetjape) Peel as Anti-Inflammation for Gingivitis after Scaling. J Heal Med Sci. 2021;4(4):24-29.
  13. Saddiq AA, Al-Ghamdi H. Aloe vera extract: A novel antimicrobial and antibiofilm against methicillin resistant Staphylococcus aureus strains. Pak J Pharm Sci. 2018;31(5(Supplementary)):2123-2130.
  14. Davis WW, Stout TR. Disc Plate Methods of Microbiological Antibiotic Assay. Microbiology. 1971;22(4):659-665.
  15. Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci. 2011;12:3422-3431.
  16. Chauhan N, Tyagi AK, Kumar P, Malik A. Antibacterial Potential of Jatropha curcas Synthesized Silver Nanoparticles against Food Borne Pathogens. Front Microbiol. 2016;7:1748. doi: 10.3389/fmicb.2016.01748.
  17. Madduluri S, Rao KB, Sitaram B. In Vitro Evaluation of Antibacterial Activity of Five Indegenous Plants Extract Against Five Bacterial Pathogens of Human. International Journal of Pharmacy and Pharmaceutical Sciences. 2013:5(4):679-684.

How to Cite

Wirata, I. N., Anak Agung Gede Agung, Ni Wayan Arini, Regina Tedja Sulaksana, Mochammad Choirul Hadi, & I Gusti Ayu Raiyanti. (2022). Antibacterial activity of Sentul fruit peel extract (Sandoricum koetjape) against Streptococcus mutans and Staphylococcus aureus. Bali Medical Journal, 11(3), 1533–1536. https://doi.org/10.15562/bmj.v11i3.3666

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I Nyoman Wirata
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Anak Agung Gede Agung
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Ni Wayan Arini
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Regina Tedja Sulaksana
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Mochammad Choirul Hadi
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I Gusti Ayu Raiyanti
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