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Effect of tartrazine on blood urea nitrogen, creatinine levels, and renal tubular necrosis in adult male Wistar rats (Rattus norvegicus): an experimental study

Abstract

Introduction: Tartrazine is still one of the most widely used coloring agents in the pharmaceutical, cosmetic, and food industries; various hazardous consequences have been identified in rodents and humans, including impaired kidney function. This current study aimed to evaluate the effect of tartrazine on the biochemical parameters and structures of the kidney.

Methods: Twenty-four adult male Wistar rats were divided into four groups of 6 each. The experimental animals received tartrazine orally at a dose of 3.75, 7.5, and 15 mg/kg body weight along with a normal diet. The control group received only food and drinking water. The study was carried out for 21 days. At the end of the experiment, biochemical (blood urea nitrogen and serum creatinine) and histopathological examinations were performed on animal kidney tissues.

Results: Our findings revealed a significant increase in urea and creatinine levels in the serum of tartrazine-treated rats compared to controls (p < 0.05). The kidney section of rats treated with tartrazine showed lumen compression of tubular cells and loss of integrity of the renal tubule membrane. Tartrazine was associated with increases in the percentage of renal tubular necrosis in a dose-dependent manner compared to the control group (p < 0.05).

Conclusions: The current study concluded that oral administration of tartrazine affected the kidney by increasing urea nitrogen, creatinine levels, and the percentage of renal tubular necrosis in male Wistar rats. The results showed that tartrazine intake could cause adverse kidney health effects.

References

  1. El-Borm H, Badawy G, Hassab El-Nabi S, El-Sherif W, Atallah M. Toxicity of sunset yellow fcf and tartrazine dyes on dna and cell cycle of liver and kidneys of the chick embryo: the alleviative effects of curcumin. Egypt J Zool [Internet]. 2020;74(74):43–55. Available from: http://dx.doi.org/10.21608/ejz.2020.42218.1040
  2. Kamal AA, Fawzia SES. Toxicological and safety assessment of tartrazine as a synthetic food additive on health biomarkers: A review. African J Biotechnol [Internet]. 2018;17(6):139–49. Available from: http://dx.doi.org/10.5897/ajb2017.16300
  3. Thurmond TS. U. S. Food and Drug Administration’s (FDA) Safety Assessment of Food Ingredients [Internet]. ACS Symposium Series. American Chemical Society; 2014. p. 91–5. Available from: http://dx.doi.org/10.1021/bk-2014-1162.ch009
  4. Silva MM, Reboredo FH, Lidon FC. Food Colour Additives: A Synoptical Overview on Their Chemical Properties, Applications in Food Products, and Health Side Effects. Foods (Basel, Switzerland) [Internet]. 2022 Jan 28;11(3):379. Available from: https://pubmed.ncbi.nlm.nih.gov/35159529
  5. El Golli N, Bini-Dhouib I, Jrad A, Boudali I, Nasri B, Belhadjhmida N, et al. Toxicity Induced after Subchronic Administration of the Synthetic Food Dye Tartrazine in Adult Rats, Role of Oxidative Stress. Recent Adv Biol Med [Internet]. 2016;02:20. Available from: http://dx.doi.org/10.18639/rabm.2016.02.284474
  6. Amchova P, Kotolova H, Ruda-Kucerova J. Health safety issues of synthetic food colorants. Regul Toxicol Pharmacol [Internet]. 2015;73(3):914–22. Available from: http://dx.doi.org/10.1016/j.yrtph.2015.09.026
  7. Steele EA, Breen C, Campbell E, Martin R. Food Regulations and Enforcement in the USA [Internet]. Reference Module in Food Science. Elsevier; 2016. Available from: http://dx.doi.org/10.1016/b978-0-08-100596-5.21031-7
  8. Scientific Opinion on the appropriateness of the food azo-colours Tartrazine (E 102), Sunset Yellow FCF (E 110), Carmoisine (E 122), Amaranth (E 123), Ponceau 4R (E 124), Allura Red AC (E 129), Brilliant Black BN (E 151), Brown FK (E 154), Brown HT (E 155. EFSA J [Internet]. 2010;8(10):1778. Available from: http://dx.doi.org/10.2903/j.efsa.2010.1778
  9. Scientific Opinion on the re-evaluation Tartrazine (E 102). EFSA J [Internet]. 2009;7(11):1331. Available from: http://dx.doi.org/10.2903/j.efsa.2009.1331
  10. Compendium of Food Additive Specifications - Joint FAO/WHO Expert Committee on Food Additives (JECFA) [Internet]. FAO and WHO; 2021. Available from: http://dx.doi.org/10.4060/cb3310en
  11. NLM PubChem CID Index [Internet]. Vitamin D Handbook. John Wiley & Sons, Inc.; p. 239–44. Available from: http://dx.doi.org/10.1002/9780470238165.indsp1
  12. Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, et al. The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat Res Toxicol Environ Mutagen [Internet]. 2002;519(1–2):103–19. Available from: http://dx.doi.org/10.1016/s1383-5718(02)00128-6
  13. Rao P, Bhat R V, Sudershan R V, Prasanna Krishna T. Consumption of synthetic food colours during festivals in Hyderabad, India. Br Food J [Internet]. 2005;107(5):276–84. Available from: http://dx.doi.org/10.1108/00070700510596875
  14. Gondokesumo ME, Amir N. The Role of Government Supervision and the Food and Drug Supervisory Agency (BPOM) in the Circulation of Counterfeit Drugs in the State of Indonesia (Judging from Law Number 36 Year 2009 and Regulation of the Head of the Drug and Food Management Agency) [Internet]. 2021;91–107. Available from: http://dx.doi.org/10.30649/ph.v21i2.16
  15. Amin KA, Abdel Hameid H, Abd Elsttar AH. Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem Toxicol [Internet]. 2010;48(10):2994–9. Available from: http://dx.doi.org/10.1016/j.fct.2010.07.039
  16. Singh P. 90 Day Repeat DOSE Oral Toxicity Study of GM Derived Cotton Seed In Wistar Rats. Am J PharmTech Res [Internet]. 2019;9(3):36–73. Available from: http://dx.doi.org/10.46624/ajptr.2019.v9.i3.003
  17. Tawfek NS, Amin HM, Abdalla AA, Fargali SHM. Adverse Effects of Some Food Additives in Adult Male Albino Rats. Current Science International. 2015;04(04):525–37.
  18. El-Wahab HMFA, Moram GSED. Toxic effects of some synthetic food colorants and/or flavor additives on male rats. Toxicol Ind Health [Internet]. 2012;29(2):224–32. Available from: http://dx.doi.org/10.1177/0748233711433935
  19. Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J basic Clin Pharm [Internet]. 2016 Mar;7(2):27–31. Available from: https://pubmed.ncbi.nlm.nih.gov/27057123
  20. Bullock G, Bunton TE. Foreword [Internet]. The Laboratory Rat. Elsevier; 2000. p. xv. Available from: http://dx.doi.org/10.1016/b978-012426400-7.50038-8
  21. Timbrell JA. Principles of Biochemical Toxicology [Internet]. CRC Press; 2008. Available from: http://dx.doi.org/10.3109/9781420007084
  22. Tarloff JB, Wallace AD. Biochemical Mechanisms of Renal Toxicity [Internet]. Molecular and Biochemical Toxicology. John Wiley & Sons, Inc.; p. 693–724. Available from: http://dx.doi.org/10.1002/9780470285251.ch29
  23. Saeid Abdelgayed S. Histopathological and immunohistochemical studies of long term administration of nutmeg on parotid salivary glands in albino rats [Internet]. Morressier; 2016. Available from: http://dx.doi.org/10.26226/morressier.578f37fdd462b8028d88fb56
  24. Usman JN, Muhammad GA. Sub-acute toxicity study on tartrazine in male albino rats. Dutse J Pure Appl Sci [Internet]. 2022;8(1b):97–105. Available from: http://dx.doi.org/10.4314/dujopas.v8i1b.12
  25. Gad SC. Rodent Models for Toxicity Testing and Biomarkers [Internet]. Biomarkers in Toxicology. Elsevier; 2019. p. 7–73. Available from: http://dx.doi.org/10.1016/b978-0-12-814655-2.00002-5
  26. Schnellmann RG. Toxic response of the kidney. In: Klaassen CD, Watkins III JB, editors. Casarett & Doulls Essentials of Toxicology. McGraw-Hill Education ; 2015. p. 209–30.
  27. Robinson L. A Practical Guide to Toxicology and Human Health Risk Assessment [Internet]. John Wiley & Sons, Inc.; 2018. Available from: http://dx.doi.org/10.1002/9781118881880
  28. Nabila M, Nawel M, Omar A, Soraya AT, Chahinaize Z, Omar K, et al. A thirteen week ad libitum administration toxicity study of Tartrazine in Swiss mice. African J Biotechnol [Internet]. 2013;12(28):4519–29. Available from: http://dx.doi.org/10.5897/ajb2013.12125
  29. Khayyat L, Essawy A, Sorour J, Soffar A. Tartrazine induces structural and functional aberrations and genotoxic effects in vivo. PeerJ [Internet]. 2017 Feb 23;5:e3041–e3041. Available from: https://pubmed.ncbi.nlm.nih.gov/28243541
  30. El-sakhawy MA, Mohamed DW, Ahmed YH. Histological and immunohistochemical evaluation of the effect of tartrazine on the cerebellum, submandibular glands, and kidneys of adult male albino rats. Environ Sci Pollut Res [Internet]. 2019;26(10):9574–84. Available from: http://dx.doi.org/10.1007/s11356-019-04399-5
  31. Cottrell S. Book Reviews : Fundamental Toxicology for Chemists Editors: John H Duffus and Howard GJ Worth The Royal Society of Chemistry 1996, 327pp. £29.50. Hum & Exp Toxicol [Internet]. 1997;16(4):237. Available from: http://dx.doi.org/10.1177/096032719701600420
  32. Lee BM, Kacew S. Lu’s Basic Toxicology [Internet]. CRC Press; 2012. Available from: http://dx.doi.org/10.3109/9781841849546
  33. Stine KE, Brown TM. Principles of Toxicology [Internet]. CRC Press; 2015. Available from: http://dx.doi.org/10.1201/b18343
  34. Balta I, Sevastre B, Mireşan V, Taulescu M, Raducu C, Longodor AL, et al. Protective effect of blackthorn fruits (Prunus spinosa) against tartrazine toxicity development in albino Wistar rats. BMC Chem [Internet]. 2019 Aug 9;13(1):104. Available from: https://pubmed.ncbi.nlm.nih.gov/31417987

How to Cite

Rahayu, M. S., Wahyuni, S. ., Fitriani, I. ., & Agung, H. B. (2022). Effect of tartrazine on blood urea nitrogen, creatinine levels, and renal tubular necrosis in adult male Wistar rats (Rattus norvegicus): an experimental study. Bali Medical Journal, 11(3), 1755–1759. https://doi.org/10.15562/bmj.v11i3.3623

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