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Grape (Vitis vinifera L.) skin extract reduced levels of SGPT and SGOT and improved the liver tissue structure of Wistar rats (Rattus novergicus) fed a high-cholesterol diet



Abstract

Background: This study determined the effect of adding grape skin extract (Vitis vinifera L.) to the diet of Wistar rats fed with high cholesterol on levels of SGPT and SGOT and the structure of liver tissue.

Methods: This study used 5 groups, namely control (standard diet), P1 (high cholesterol diet), P2 (high cholesterol diet and extract of grape skin as much as 100 mg/200 gBW/day), P3 (high cholesterol diet and extract of grape skin as much as 250 mg/200 gBW/day) and P4 (high cholesterol diet and extract of grape skin 500 mg/200 gBW/day). The study began with the production of extracts, the extracts dosage determination, high cholesterol diet preparation, sample (animals) preparation, extract addition to animals, SGPT and SGOT levels measurement, rat liver histopathology preparation and histopathological features observation, then ended with data analysis.

Results: The results showed that administration of grape skin extract in Wistar rats fed a high-cholesterol diet affected the levels of SGPT and SGOT, as well as parenchymatous degeneration of hepatocytes. Administration of grape skin extract as much as 500 mg/200 g BW/day had the lowest mean levels of SGPT and SGOT and the lowest parenchymatous degeneration of hepatocytes.

Conclusion: Thus, the extract of grape skin (Vitis vinifera L.) has the potential of hepatoprotector because it contains anthocyanin, which functions as an antioxidant and protects against liver damage.

Keywords: anthocyanin grape skin extract hepatoprotector high-cholesterol diet

References

  1. Nasution LS. Pengaruh pemberian likopen terhadap perkembangan lesi aterosklerosis pada tikus hiperkolesterolemia.Jurnal Kedokteran dan Kesehatan 2013;9:2.
  2. Sudoyo AW, Setiyohadi B, Alwi I, et al. Buku ajar ilmu penyakit dalam jilid II. Fifth ed. Jakarta: Interna Publishing. 2009.
  3. Rufaida F, Aulanni’am, Murwani S. Profil kadar kolesterol total, low density lipoprotein (LDL), dan gambaran histopatologi aorta pada tikus (Rattus norvegicus) hiperkolesterolemia dengan terapi ekstrak air benalu mangga (Dendropthoe pentandra). Malang: Brawijaya University. 2012.
  4. Zhang Q, Lu L. Non alcoholic fatty liver disease:Dyslipidemia, risk for cardiovascular complications, and treatment strategy. J Clin Transl Hepatol. 2015;3:78–84.
  5. Bellentani S, Marino M. Epidemiology and natural history of non-alcoholic fatty liver disease (NAFLD). Ann Hepatol. 2009;8:S4-S8.
  6. Sattar N, Forrest E, Preiss D. Non-alcoholic fatty liver disease. BMJ. 2014;349:g4596.
  7. Komosinska-Vassev K, Olczyk P, Kafmierczak J. Bee pollen: Chemical composition and therapeutic application. Evid Based Complement Alternat Med. 2015;2015:1-6.
  8. Laili U. Pengaruh pemberian temulawak (Curcuma xanthorrhiza Roxb) dalam bentuk kapsul terhadap kadar SGPT (Serum Glutamat Piruvat Transaminase) dan SGOT (serum Glutamat Oksaloasetat Transaminase) pada orang sehat. Yogyakarta: Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta. 2013.
  9. Widarti, Nurqaidah. Analisis kadar serum glutamic pyruvic transaminase (SGPT) dan serum glutamic oxaloacetic transaminase (SGOT) pada petani yang menggunakan pestisida. Jurnal Media Analis Kesehatan. 2019;10:35-43.
  10. Wijekoon C, Netticadan T, Siow YL, Sabra A, Yu L, Raj P, et al. Potential Associations among Bioactive Molecules, Antioxidant Activity and Resveratrol Production in Vitis vinifera Fruits of North America. Molecules. 2022;27(2):336.
  11. Maryam S. Total phenol, flavonoid levels and IC50 in local grape (Vitis vinifera L) skin waste wine. In: Proceedings of the 7th Mathematics, Science, and Computer Science Education International Seminar. Bandung: EAI; 2019
  12. Devi S, Singh R. Antioxidant and anti- hypercholesterolemic potential of Vitis vinifera leaves. Pharmacognosy Journal. 2017;9:807-814.
  13. Giribabu N, Kumar KE, Rekha SS, et al. Vitis vinifera (muscat variety) seed ethanolic extract preserves activity levels of enzymes and histology of the liver in adult male rats with diabetes. Evidence-Based Complementary and Alternative Medicine. 2015;2015:1-8.
  14. Malloy M, Kane J. Agents used in dyslipidemia. In: Katzung B, Masters S, Trevor A, editors. Basic and Clinical Pharmacology. New York: McGraw-Hill; 2012. p.619-634.
  15. Ngatidjan PS. Farmakologi dasar. Yogyakarta: FK UGM. 2006.
  16. Kurniawati I, Nurmasitoh T, Yahya TN. Effect of giving ethanol multistep doses to level of SGPT and SGOT in wistar rats (Rattus norvegicus). Indonesian Journal of Medicine and Health. 2015;7:30-35.
  17. Abdelbaky M sabry, Ibrahim HS, Hassan ML, Sayed ZE. Nanoparticles Effects of Red Grape (Vitis vinifera) Seeds and Grape Seeds Powder on Obese Hyperlipidemic Rats. ARC J Nutr Growth. 2016;2(2):1–15.
  18. Nassiri-Asl M, Hosseinzadeh H. Review of the Pharmacological Effects of Vitis vinifera (Grape) and its Bioactive Constituents: An Update. Phytother Res. 2016;30(9):1392–403.
  19. Zubaidah E, Veronica C. Studi aktivitas antioksidan cuka berbasis buah anggur bali (Vitis vinifera) utuh dan tanpa kulit. Jurnal Teknologi Hasil Pertanian. 2014;7: 95-103.
  20. Ghaffar S, Naqvi MA, Fayyaz A, et al.What is the influence of grape products on liver enzymes? A systematic review and meta-analysis of randomized controlled trials. Complementary Therapies in Medicine. 2022;69:1-7.
  21. Sarkhosh-Khorasani S, Sangsefidi ZS, Hosseinzadeh M. The effect of grape products containing polyphenols on oxidative stress: a systematic review and meta-analysis of randomized clinical trials. Nutr J. 2021;20(25).
  22. Bak MJ, Truong V-L, Ko S-Y, Nguyen XNG, Ingkasupart P, Jun M, et al. Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats. Int J Mol Sci. 2016;17(5):785.
  23. Okaiyeto K, Nwodo UU, Mabinya L V., Okoh AI. A Review on Some Medicinal Plants with Hepatoprotective Effects. Pharmacogn Rev. 2018;12(24):186–99.
  24. Handani KS, Utami WS, Hermansyah B, et al. Gambaran histopatologi hati tikus wistar pasca pemberian ekstrak etanol rimpang bangle (Zingiber cassumunar Roxb.) pada uji toksisitas akut. Journal of Agromedicine and Medical Sciences. 2018;4:55-59.
  25. Dwinanda A, Afriani N, Hardisman. Pengaruh jus seledri (Apium graveolens L.) terhadap gambaran mikroskopis hepar tikus (Rattus norvegicus) yang diinduksi diet hiperkolesterol, Jurnal Kesehatan Andalas. 2019;8:68-75.
  26. Prahastuti S, Ladi JE, Dewi K, et al. The effect of bee pollen on SGOT, SGPT levels and liver histopathological images of male rats wistar induced by high fat diet. J Med Health.2020;2(5):51-60.
  27. Ramadhani FQ, Suryani D. Comparison of the Effectiveness of Hepatoprotectors of Black Cumin Extract and Temulawak Extract in SGOT and SGPT Induced by Paracetamol. JIMKI J Ilm Mhs Kedokt Indones. 2020;8(2):29–35.
  28. Bairagi S, Ghule P, Gilhotra R. Molecular Docking, In vitro Antioxidant, and In vivo Hepatoprotective Activity of Methanolic Extract of Calotropis gigantea leaves in Carbon Tetrachloride-induced Liver Injury in Rats. Curr Enzym Inhib. 2022;18(2):110–26.

How to Cite

Siti Maryam, Ni Kadek Alit Arsani, & Sartika Tangguda. (2022). Grape (Vitis vinifera L.) skin extract reduced levels of SGPT and SGOT and improved the liver tissue structure of Wistar rats (Rattus novergicus) fed a high-cholesterol diet. Bali Medical Journal, 11(3), 1404–1408. https://doi.org/10.15562/bmj.v11i3.3602
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