Effect of secretome of adipose stem cell (ASC) in photoaging skin

Winawati Eka Putri; Anang Endaryanto; Damayanti Tinduh; Fedik Rantam; Hari Basuki Notobroto; Cita Rosita Sigit Prakoeswa

doi:10.15562/bmj.v11i3.3543

Effect of secretome of adipose stem cell (ASC) in photoaging skin

Winawati Eka Putri ,

Anang Endaryanto ,

Damayanti Tinduh ,

Fedik Rantam ,

Hari Basuki Notobroto ,

Cita Rosita Sigit Prakoeswa ,

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DOI: https://doi.org/10.15562/bmj.v11i3.3543 |
Published: 2022-09-15

Abstract

Introduction: UV light is the principal cause of photoaging, a kind of aging. The problem arises because it is less enjoyable. Utilizing the secretome of adipose stem cells (ASC), which is rich in growth factors, could be a novel anti-photoaging therapy. The secretome of ASCs has been extensively examined for their role in wound healing, but their function in photoaging has just lately been investigated. This study looked at changes in epidermal and dermal thickness, expression of MMP-1 and TIMP-1, as well as dermal collagen density to assess the influence of the secretome of ASC in photoaging treatment.

Methods: 28 male Wistar rats, 10-12 weeks old, were randomly assigned to one week of acclimation in each of four groups: UV-irradiated group with topical secretome of ASC (Group P1); UV-irradiated group with topical vehicle only (Group P2); only topical secretome of ASC, non-UV-irradiated (Group P3); and non-UV-irradiated and no topical treatment (Group P4) (Group P0). Prior to treating the rats, the UV exposure was administered for six weeks. Then, for a period of four weeks, the secretome ASC was administered as a treatment.

Results: The epidermal and dermal thickness were significantly improved after four weeks treatment compared to UV irradiated with topical vehicle only group (p<0.05). The expression of TIMP-1 and dermal collagen density were significantly increased (p<0.05).

Conclusion: Secretome of ASC may be a solution for photoaging skin.

References

Chung J, Cho S, Kang S. Why Does the Skin Age? Intrinsic Aging, Photoaging, and Their Pathophysiology. New York: Marcel Dekker; 2004.
Amaro-Ortiz A, Yan B, D’Orazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules. 2014;19(5):6202–19.
Garg C, Khurana P, Garg M. Molecular mechanisms of skin photoaging and plant inhibitors. Int J Green Pharm. 2017;11(2):S217–32.
Amano S. Characterization and mechanisms of photoageing-related changes in skin. Damages of basement membrane and dermal structures. Exp Dermatol. 2016;25 Suppl 3:14–9.
Alam M, Havey J. Photoaging. In: Cosmetic Dermatology: Products and Procedures. Wiley Online Library; 2010. p. 13–21.
Garmyn M, Oord J. Clinical and histological changes of photoaging. In: Photoaging. Marcel Dekker; 2004. p. 33–54.
Rangarajan V, Dreher F. Topical growth factors for skin rejuvenation. In: Textbook of Aging Skin. 2010.
Fitzpatricks R. Use of Growth Factors in Cosmeceuticals. In: Cosmeceuticals and Active Cosmetics drugs versus cosmetics. USA: Taylor & Francis Group; 2005.
Shabunin AS, Yudin VE, Dobrovolskaya IP, Zinovyev E V., Zubov V, Ivan’kova EM, et al. Composite wound dressing based on chitin/chitosan nanofibers: Processing and biomedical applications. Cosmetics. 2019;6(1).
Choi JS, Cho WL, Choi YJ, Kim JD, Park H-A, Kim SY, et al. Functional recovery in photo-damaged human dermal fibroblasts by human adipose-derived stem cell extracellular vesicles. J Extracell vesicles. 2019;8(1):1565885.
Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis. Int J Mol Sci. 2016;17(6).
Kim W-S, Park B-S, Sung J-H. Protective role of adipose-derived stem cells and their soluble factors in photoaging. Arch Dermatol Res. 2009;301(5):329–36.
Yaar M. Clinical and histological features of intrinsic versus extrinsic skin aging. In: Skin Aging. 2006.
Yaar M, Gilchrest B. Aging of skin. In: Fitzpatrick’s dermatology in general medicine. 8th ed. New York: McGraw-Hill Companies Inc; 2012.
Mehta RC, Fitzpatrick RE. Endogenous growth factors as cosmeceuticals. Dermatol Ther. 2007;20(5):350–9.
Amirthalingam M, Bhat S, Dighe P, Setharm R. Human Mesenchymal Stromal Cells-Derived Conditioned Medium Based Formulation for Advanced Skin Care: in vitro and in vivo Evaluation. J Stem Cells Res Dev Ther. 2019;5(February):1–8.
Kim KH, Kim Y-S, Lee S, An S. The effect of three-dimensional cultured adipose tissue-derived mesenchymal stem cell–conditioned medium and the antiaging effect of cosmetic products containing the medium. Biomed Dermatology. 2020;4(1):1–12.
Kim HJ, Jung MS, Hur YK, Jung AH. A study on clinical effectiveness of cosmetics containing human stem cell conditioned media. Biomed Dermatology. 2020;4(1):1–11.
Chen S, He Z, Xu J. Application of adipose-derived stem cells in photoaging: basic science and literature review. Stem Cell Res Ther. 2020;11(1):1–15.
Garmyn M, Yarosh DB. The molecular and genetic effects of ultraviolet radiation exposure on skin cells. In: Photodermatology. New York: Marcel Dekker; 2007. p. 41.
Quan T, Qin Z, Xia W, Shao Y, Voorhees JJ, Fisher GJ. Matrix-degrading metalloproteinases in photoaging. J Investig dermatology Symp Proc. 2009;14(1):20–4.
Shin J-W, Kwon S-H, Choi J-Y, Na J-I, Huh C-H, Choi H-R, et al. Molecular Mechanisms of Dermal Aging and Antiaging Approaches. Int J Mol Sci. 2019;20(9):2126.
Yokose U, Hachiya A, Sriwiriyanont P, Fujimura T, Visscher MO, Kitzmiller WJ, et al. The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage. J Invest Dermatol. 2012;132(12):2800–9.
Pricilla E, Veraida A, Julianto I, Kariosentono H, Budiani DR, Fibrianto YH, et al. The effect of injection of ADSC compared to APPE on collagen density in aging skin (animal study). J Gen Dermatology Venereol Indones. 2017;2(2):58–63.

[1] Chung J, Cho S, Kang S. Why Does the Skin Age? Intrinsic Aging, Photoaging, and Their Pathophysiology. New York: Marcel Dekker; 2004.

[2] Amaro-Ortiz A, Yan B, D’Orazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules. 2014;19(5):6202–19.

[3] Garg C, Khurana P, Garg M. Molecular mechanisms of skin photoaging and plant inhibitors. Int J Green Pharm. 2017;11(2):S217–32.

[4] Amano S. Characterization and mechanisms of photoageing-related changes in skin. Damages of basement membrane and dermal structures. Exp Dermatol. 2016;25 Suppl 3:14–9.

[5] Alam M, Havey J. Photoaging. In: Cosmetic Dermatology: Products and Procedures. Wiley Online Library; 2010. p. 13–21.

[6] Garmyn M, Oord J. Clinical and histological changes of photoaging. In: Photoaging. Marcel Dekker; 2004. p. 33–54.

[7] Rangarajan V, Dreher F. Topical growth factors for skin rejuvenation. In: Textbook of Aging Skin. 2010.

[8] Fitzpatricks R. Use of Growth Factors in Cosmeceuticals. In: Cosmeceuticals and Active Cosmetics drugs versus cosmetics. USA: Taylor & Francis Group; 2005.

[9] Shabunin AS, Yudin VE, Dobrovolskaya IP, Zinovyev E V., Zubov V, Ivan’kova EM, et al. Composite wound dressing based on chitin/chitosan nanofibers: Processing and biomedical applications. Cosmetics. 2019;6(1).

[10] Choi JS, Cho WL, Choi YJ, Kim JD, Park H-A, Kim SY, et al. Functional recovery in photo-damaged human dermal fibroblasts by human adipose-derived stem cell extracellular vesicles. J Extracell vesicles. 2019;8(1):1565885.

[11] Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis. Int J Mol Sci. 2016;17(6).

[12] Kim W-S, Park B-S, Sung J-H. Protective role of adipose-derived stem cells and their soluble factors in photoaging. Arch Dermatol Res. 2009;301(5):329–36.

[13] Yaar M. Clinical and histological features of intrinsic versus extrinsic skin aging. In: Skin Aging. 2006.

[14] Yaar M, Gilchrest B. Aging of skin. In: Fitzpatrick’s dermatology in general medicine. 8th ed. New York: McGraw-Hill Companies Inc; 2012.

[15] Mehta RC, Fitzpatrick RE. Endogenous growth factors as cosmeceuticals. Dermatol Ther. 2007;20(5):350–9.

[16] Amirthalingam M, Bhat S, Dighe P, Setharm R. Human Mesenchymal Stromal Cells-Derived Conditioned Medium Based Formulation for Advanced Skin Care: in vitro and in vivo Evaluation. J Stem Cells Res Dev Ther. 2019;5(February):1–8.

[17] Kim KH, Kim Y-S, Lee S, An S. The effect of three-dimensional cultured adipose tissue-derived mesenchymal stem cell–conditioned medium and the antiaging effect of cosmetic products containing the medium. Biomed Dermatology. 2020;4(1):1–12.

[18] Kim HJ, Jung MS, Hur YK, Jung AH. A study on clinical effectiveness of cosmetics containing human stem cell conditioned media. Biomed Dermatology. 2020;4(1):1–11.

[19] Chen S, He Z, Xu J. Application of adipose-derived stem cells in photoaging: basic science and literature review. Stem Cell Res Ther. 2020;11(1):1–15.

[20] Garmyn M, Yarosh DB. The molecular and genetic effects of ultraviolet radiation exposure on skin cells. In: Photodermatology. New York: Marcel Dekker; 2007. p. 41.

[21] Quan T, Qin Z, Xia W, Shao Y, Voorhees JJ, Fisher GJ. Matrix-degrading metalloproteinases in photoaging. J Investig dermatology Symp Proc. 2009;14(1):20–4.

[22] Shin J-W, Kwon S-H, Choi J-Y, Na J-I, Huh C-H, Choi H-R, et al. Molecular Mechanisms of Dermal Aging and Antiaging Approaches. Int J Mol Sci. 2019;20(9):2126.

[23] Yokose U, Hachiya A, Sriwiriyanont P, Fujimura T, Visscher MO, Kitzmiller WJ, et al. The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage. J Invest Dermatol. 2012;132(12):2800–9.

[24] Pricilla E, Veraida A, Julianto I, Kariosentono H, Budiani DR, Fibrianto YH, et al. The effect of injection of ADSC compared to APPE on collagen density in aging skin (animal study). J Gen Dermatology Venereol Indones. 2017;2(2):58–63.

Effect of secretome of adipose stem cell (ASC) in photoaging skin

Abstract

References

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