Anti-inflammatory effects and potential mechanisms of Mitragyna speciosa methanol extract on λ-karagenan-induced inflammation model

Hotimah Masdan  Salim; Choirotussanijjah; Evi Sylvia  Awwalia; Ilham Putera  Alam

doi:10.15562/bmj.v11i3.3535

Anti-inflammatory effects and potential mechanisms of Mitragyna speciosa methanol extract on λ-karagenan-induced inflammation model

Hotimah Masdan Salim ,

Choirotussanijjah ,

Evi Sylvia Awwalia ,

Ilham Putera Alam ,

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DOI: https://doi.org/10.15562/bmj.v11i3.3535 |
Published: 2022-09-13

Abstract

Introduction: Mitragyna speciosa is known to have beneficial effects, such as antinociceptive and anti-inflammatory effects. There are some components that involve in inflammatory, such as Inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) enzymes, and also cytokines. This study aimed to investigate the anti-inflammatory effect and its mechanism of Mitragyna speciosa leaf.

Methods: Carrageenan-induced rat paw edema model was used for inflammation induction. Rats were given Mitragyna speciosa leaf methanol extract at a dose of 75, 150, and 200 mg/kg.

Results: Intraplantar injection of carrageenan led to the development of time-dependent peripheral inflammation, which, in turn, was a significant increase in the swelling process as measured by a plethysmometer. However, administration of EMS (75,150 and 200mg/kg) can reduce edema in a dose-dependent manner. We also demonstrated that skin histology on EMS administration significantly preserved skin thickness at 4 h after carrageenan injection.

Conclusion: In conclusion, we reveal the role of Mytragyna speciosa methanol extract as anti-inflammatory and maintain skin thickness in the inflammatory process.

References

Abd-Allah AAM, El-Deen NAMN, Mohamed WAM, Naguib FM. Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema. Iran J Basic Med Sci. 2018;21(1):97.
Lee HJ, Seo H, Kim G, Jeon CY, Park JH, Jang B, et al. Houttuynia cordata Thunb inhibits the production of pro‑inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC‑1 human mast cells. Mol Med Rep. 2013;8(3):731–6.
Abbate GM, Sacerdote P, Amodeo G, Mangano A, Levrini L. Experimentally Induced Pulpal Lesion and Substance P Expression: Effect of Ketoprofen—A Preliminary Study. Int J Dent. 2016;2016.
Biebl MM, Buchner J. Structure, Function, and Regulation of the Hsp90 Machinery. Cold Spring Harb Perspect Biol. 2019;11(9):a034017.
Al-Wajeeh NS, Hajerezaie M, Noor SM, Halabi MF, Al-Henhena N, Azizan AHS, et al. The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats. BMC Vet Res. 2017;13(1):27.
Swogger MT, Hart E, Erowid F, Erowid E, Trabold N, Yee K, et al. Experiences of kratom users: a qualitative analysis. J Psychoactive Drugs. 2015;47(5):360–7.
Canene-Adams K. Chapter Fifteen - Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry. In: Lorsch JBT-M in E, editor. Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate. Academic Press; 2013. p. 225–33.
Kleczek P, Jaworek-Korjakowska J, Gorgon M. A novel method for tissue segmentation in high-resolution H&E-stained histopathological whole-slide images. Comput Med Imaging Graph. 2020;79:101686.
Myers MJ, Deaver CM, Lewandowski AJ. Molecular mechanism of action responsible for carrageenan-induced inflammatory response. Mol Immunol. 2019;109:38–42.
McKim JM, Baas H, Rice GP, Willoughby JA, Weiner ML, Blakemore W. Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines. Food Chem Toxicol. 2016;96:1–10.
Jazzara M, Ghannam A, Soukkarieh C, Murad H. Anti-Proliferative Activity of λ-Carrageenan Through the Induction of Apoptosis in Human Breast Cancer Cells. Iran J cancer Prev. 2016;9(4):e3836–e3836.
Utar Z, Majid MIA, Adenan MI, Jamil MFA, Lan TM. Mitragynine inhibits the COX-2 mRNA expression and prostaglandin E2 production induced by lipopolysaccharide in RAW264.7 macrophage cells. J Ethnopharmacol. 2011;136(1):75–82.

[1] Abd-Allah AAM, El-Deen NAMN, Mohamed WAM, Naguib FM. Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema. Iran J Basic Med Sci. 2018;21(1):97.

[2] Lee HJ, Seo H, Kim G, Jeon CY, Park JH, Jang B, et al. Houttuynia cordata Thunb inhibits the production of pro‑inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC‑1 human mast cells. Mol Med Rep. 2013;8(3):731–6.

[3] Abbate GM, Sacerdote P, Amodeo G, Mangano A, Levrini L. Experimentally Induced Pulpal Lesion and Substance P Expression: Effect of Ketoprofen—A Preliminary Study. Int J Dent. 2016;2016.

[4] Biebl MM, Buchner J. Structure, Function, and Regulation of the Hsp90 Machinery. Cold Spring Harb Perspect Biol. 2019;11(9):a034017.

[5] Al-Wajeeh NS, Hajerezaie M, Noor SM, Halabi MF, Al-Henhena N, Azizan AHS, et al. The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats. BMC Vet Res. 2017;13(1):27.

[6] Swogger MT, Hart E, Erowid F, Erowid E, Trabold N, Yee K, et al. Experiences of kratom users: a qualitative analysis. J Psychoactive Drugs. 2015;47(5):360–7.

[7] Canene-Adams K. Chapter Fifteen - Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry. In: Lorsch JBT-M in E, editor. Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate. Academic Press; 2013. p. 225–33.

[8] Kleczek P, Jaworek-Korjakowska J, Gorgon M. A novel method for tissue segmentation in high-resolution H&E-stained histopathological whole-slide images. Comput Med Imaging Graph. 2020;79:101686.

[9] Myers MJ, Deaver CM, Lewandowski AJ. Molecular mechanism of action responsible for carrageenan-induced inflammatory response. Mol Immunol. 2019;109:38–42.

[10] McKim JM, Baas H, Rice GP, Willoughby JA, Weiner ML, Blakemore W. Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines. Food Chem Toxicol. 2016;96:1–10.

[11] Jazzara M, Ghannam A, Soukkarieh C, Murad H. Anti-Proliferative Activity of λ-Carrageenan Through the Induction of Apoptosis in Human Breast Cancer Cells. Iran J cancer Prev. 2016;9(4):e3836–e3836.

[12] Utar Z, Majid MIA, Adenan MI, Jamil MFA, Lan TM. Mitragynine inhibits the COX-2 mRNA expression and prostaglandin E2 production induced by lipopolysaccharide in RAW264.7 macrophage cells. J Ethnopharmacol. 2011;136(1):75–82.

Anti-inflammatory effects and potential mechanisms of Mitragyna speciosa methanol extract on λ-karagenan-induced inflammation model

Abstract

References

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