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Depot Medroxyprogesterone acetate reduces spermatogonia cells and spermatid cells in the seminiferous tubules of male mice


Background: Until now the existing male contraceptive methods are periodic abstinence, interrupted intercourse (coitus interruptus), the use of condoms, and vasectomy. Another alternative method of contraception for men is the use of the hormone depot medroxyprogesterone acetate (DMPA) as has been done in female contraceptives. Research with DMPA at a dose of 1.25 mg was effective in reducing the concentration and viability of spermatozoa and testosterone secretion in male rats.

Aim: to discover if DMPA as a gonadotropin hormon could effectively inhibit the process of spermatogenesis in male mice.

Methods: This research is an experimental research with post-test control group design. A total of 36 samples were grouped by simple random sampling into two groups. Control group (P0): consisted of 18 male mice given 0.3 ml of intramuscular aquabides. Treatment group (P1): 18 male mice were given DMPA intramuscularly (IM) with a dose of 3 mg as much as 0.3 ml. Data obtained from examination of the mean of 60 tubules (30 tubules from the right testis and 30 tubules from the left testis) for each treatment with HE staining at 400x magnification.

Results: The results showed a significant difference in the decrease in the number of spermatogonia cells in the treatment group compared to the control group (9.83±2.33 vs 12.78±1.67 cells/tubule), with p value = 0.000 (p<0.01) and Spermatid cells (76.50±7.66 vs 86.44±6.06 cells/tubule), with p value = 0.000 (p<0.01). The administration of DMPA could significantly reduce the number of spermatogonia and spermatid cells in the seminal tubules of male mice.

Conclusion: The results of the study have implications for further research on the use of DMPA as a male contraceptive in higher experimental animals such as rabbits, then clinical trials in humans.


  1. Yurnadi, Suryandari, D,A., Moeloek, N.Pengaruh penyuntikan dosis minimal depo medroksiprogesteron asetat (DMPA) terhadap berat badan dan kimia darah tikus jantan galur Sprague-dawley. 2009. 13(2):189-194.
  2. Moeloek, N. Kontrasepsi Hormonal Pada Pria. Pertemuan Ilmiah Tahunan Persandi V-Pandi XIX, dengan judul Current Hopes in Sexual Dysfunction and Infertility Solution. 27-29 April. Grand Sahid Jaya Hotel Jakarta. 2011.
  3. Proporsi metode kontrasepsi menurut jenis kelamin, BKKBN. 2014. Dapat diakses di infodatin- harganas.pdf
  4. Biro Pusat Statistik, Badan Koordinasi Keluarga Berencana Nasional, Depkes, Macro International Inc, Survei Demografi dan Kesehatan Indonesia, 2011: XXIV+228
  5. Sumaryati, A. “Tahun Ini KB Pria Mulai Digalakkan”. Badan Koordinator Keluarga Berencana Nasional. 2004. Available from: Accessed, 26, Oct. 2011.
  6. Hartono, H. “Keluarga Berencana dan Kontrasepsi.” Pustaka Sinar Harapan. Jakarta. 1996.
  7. Tjokronegoro, A. “Penyebab dan Patofisiologi Infertilitas Pria.” Pertemuan Ilmiah. 2002.
  8. Johnson, M. and Eviritt. “Essensial Reproduction.” 3 nd Edition. Blackwell Sci. Pub. 1990.
  9. Matsumoto, A..M. “Spermatogenesis” In: Adashi, E.Y., Rock, J.A. Zev. 1996
  10. Charan J, Kantharia ND. How to calculate sample size in animal studies?. J Pharmacol Pharmacother. 2013;4(4):303-306.
  11. Turek, P.J. “Hypothalamic-Pituitary-Gonadal (HPG) Axis and Control of Spermatogenesis”. In: Endocrine Evaluation. Male Reproductive Laboratory Departement of Uroligy Universitas of California at San Francisco. San Francisco, California. 2005.
  12. Ilyas S. Effect of Methanolic Momordica charantia Seed Extract and Depot Medroxyprogesterone Acetate (DMPA) to Quantity and Quality of Rat Sperm. International Journal of Pharm Tech Research, 2014;6(6):1818.
  13. Kansil SE, Kundre R, Bataha Y. Hubungan Penggunaan Kontrasepsi Suntik Depo Medroksiprogesteron Asetat (DMPA) dengan Perubahan Fisiologis pada Wanita Usia Subur (WUS) di Puskesmas Ranomuut Kota Manado. EJournal Keperawatan (Ekp). 2015;3(3):4-6.

How to Cite

Bagus Komang Satriyasa, I Gusti Ayu Widianti, & I.B.G. Fajar Manuaba. (2022). Depot Medroxyprogesterone acetate reduces spermatogonia cells and spermatid cells in the seminiferous tubules of male mice. Bali Medical Journal, 11(1), 508–512.




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Bagus Komang Satriyasa
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I.B.G. Fajar Manuaba
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