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Application of Moringa Oleifera leaves extract cream inhibits paw edema in white male Wistar rat (Rattus norvegicus) induced by carrageenan 1%



Abstract

Introduction: Inflammation is the body's response to body tissue injury characterized by swelling or edema. Moringa oleifera is known to have many benefits as herbal medicine. Moringa oleifera leaves have been shown to have anti-inflammatory activity. The polyphenol content in Moringa leaves, such as flavonoids and tannins, has been observed as a potential anti-inflammatory.

Methods: Thirty white male Wistar rats were randomly divided into five groups. Group 0 (negative control group) was given base cream, group 1 (positive control) was given 2.5% hydrocortisone cream, group 2 was given 10% moringa leaves extract cream, group 3 was given 12% moringa leaves extract cream, and group 4 was given 14% moringa leaves extract cream. One hour after treatment was applied in the plantar area, all mice were induced with 0.1 ml of 1% carrageenan. The thickness of the rat paw edema was measured every 1 hour for 6 hours using a caliper.

Results: This study showed a significant difference in rat paw edema between the base cream and the entire treatment groups (p<0.05). There was no significant difference between the 2.5% hydrocortisone cream group and the 10%, 12%, and 14% moringa leaves extract cream group (p>0.05). The 14% cream extract had the largest paw edema inhibition among other moringa leaves extract creams which were 27.4%.

Conclusion: This study concludes that 10%, 12%, and 14% of moringa leaves extract cream have anti-inflammatory effects with higher concentrations resulting in better efficacy.
Keywords: anti-inflammatory moringa leaf extract cream carrageenan rat paw edema

References

  1. Khedir BS, Mzid M, Bardaa S, et al. In vivo evaluation of the anti-inflammatory effect of Pistacia lentiscus fruit oil and its effects on oxidative stress. Evidence-Based Complementary and Alternative Medicine.2016;1-12.
  2. Bellomo R, Carmignano S, Palermo T, et al. Nonsteroidal Anti-inflammatory Drugs: Integrated Approach to Physical Medicine and Rehabilitation. London: IntechOpen Limited. 2017.
  3. Stankov S. Definition of Inflammation, Causes of Inflammation and Possible Anti-inflammatory Strategies. The Open Journal. 2012;5:1-9
  4. Varshney I, Amin SS, Adil M, et al. Topical Corticosteroid Abuse- Risk factors and Consequences. JDA Indian Journal of Clinical Dermatology. 2019;2(72):1-7.
  5. Prabu SL, Umamaheswari A, Puratchikody A. Phytopharmacological potential of the natural gift Moringa oleifera Lam and its therapeutic application: An overview. Asian Pacific Journal of Tropical Medicine. 2019;12(11), 485.
  6. Kementrian Kesehatan RI. Formularium Obat Herbal Asli Indonesia. Volume 1. Jakarta: Kementrian Kesehatan RI. 2011.
  7. Rajbhar YP, Rajbhar G, Rawat PL, et al. Grow Moringa (Moringa oleifera), the miracle tree on the earth. Horticulture International Journal. 2018;2(4):166-172.
  8. Arulselvan P, Fard MT, Tan WS, et al. Role of Antioxidants and Natural Products in Inflammation. Oxid Med Cell Longev. 2016:1-15
  9. Ulfa M, Hendrarti W, Muhram PN. Formulasi Gel Ekstrak Daun Kelor (Moringa oleifera Lam.) Sebagai Anti Inflamasi Topikal Pada Tikus (Rattus novergicus). J. Pharm. Med. Sci. 2016;1: 30-35.
  10. Nanaryain MF. Efek antiinflamasi sediaan krim ekstrak etanol daun kelor (moringa oleifera lam.) Pada tikus putih jantan yang diinduksi karagenin. Surakarta: Universitas Setiabudi. 2016.
  11. Husni P, Pratiwi, AN, Baitariza A. Formulasi Krim Ekstrak Etanol Daun Kelor (Moringa oleifera Lamk). Jurnal Ilmiah Farmasi Farmasyifa, 2019;2(2):101-110
  12. Federer WT. Statistical Design and Analysis for Intercropping Experiments. New York: Springer. 2008:30-33.
  13. Hendra P, Fenty APR, Pangestuti BM, et al. Evaluation of Antihyperlipidemic, Anti-inflammatory, and Analgesic Activities of Eurycoma longifolia in Animal Models. International Journal of Pharmacy and Pharmaceutical Sciences. 2017;9(3):1-4.
  14. Jamal S, Anwar Y. Uji ?ktivit?s ?ntiinfl?m?si Miny?k Gosok Miny?k ?tsiri Ser?i W?ngi (Cympobogon N?rdus) Terh?d?p Tikus Putih J?nt?n (R?ttus norvegicus). Indonesia Natural Research Pharmaceutical Journal. 2018;3(2):69-82.
  15. Katzung BG. Basic & Clinical Pharmacology. 10th ed. New York: McGraw-Hill Companies. 2012:45-50.
  16. Uva L, Miguel D, Pinheiro C, et al. Mechanisms of action of topical corticosteroids in psoriasis. Int J Endocrinol. 2012;1-16
  17. Yamamoto Y and Gaynor RB. Therapeutic potential of inhibition of the NF-?B pathway in the treatment of inflammation and cancer. The Journal of clinical investigation. 2001;107(2):135-142.
  18. Nieman D. Quercetin's Influence on Exercise-Induced Changes in Plasma Cytokines and Muscle and Leukocyte Cytokine mRNA. Journal of Applied Physiology, 103(5):1728-1735.
  19. Sulistyawati R, and Pratiwi PY. Pengaruh pemberian ekstrak etanol daun kelor (Moringa oleifera l.) terhadap aktivitas analgesik dan antiinflamasi melalui ekspresi enzim siklooksigenase. Pharmaciana. 2016;6(1):31-38
  20. Leone A, Spada A, Battezzati A, et al. 2015. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International journal of molecular sciences. 2015;16(6):12791-12835.
  21. Ulvian F and Yusriadi KK. Pengaruh Gel Ekstrak Daun Sirih Merah (Piper crocatum Ruiz &Pav) Terhadap Penyembuhan Luka Bakar Pada Kelinci. Galenika Journal of Pharmacy; 2016;2(2): 103-110.
  22. Erwiyani AR, Haswan D, Agasi A, et al. Pengaruh sediaan gel dan krim ekstrak etanol daun kelor (Moringa oleifera Lamk) terhadap penurunan luas luka bakar pada tikus. Indonesian Journal of Pharmacy and Natural Product. 2020;3(2).
  23. Lee S, Lee I, Mar W. Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 activity by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose in murine macrophage cells. Arch. Pharm. Res. 2003;26:832-839.
  24. Yeo J, Lee J, Yoon S, et al. Tannic acid-based nanogel as an efficient anti-inflammatory agent. Biomaterials science. 2020;8(4):1148-1159.
  25. Park JW, Choi YJ, Suh SI, et al. Involvement of ERK and protein tyrosine phosphatase signaling pathways in EGCG-induced cyclooxygenase-2 expression in Raw 264.7 cells. Biochem. Biophys. Res. Commun. 2001;286: 721-725.

How to Cite

Darmaputra, I. G. N., Pramita, I. G. A. S., & Winaya, K. K. (2022). Application of Moringa Oleifera leaves extract cream inhibits paw edema in white male Wistar rat (Rattus norvegicus) induced by carrageenan 1%. Bali Medical Journal, 11(1), 122–126. https://doi.org/10.15562/bmj.v11i1.3384
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I Gusti Nyoman Darmaputra
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I Gusti Ayu Sattwika Pramita
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Ketut Kwartantaya Winaya
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