Bioinformatics assessment on the potential of Lipoteichoic Acid (LTA) of Lactic Acid Bacteria (LAB) as topical therapy for inflammatory skin diseases

Radityastuti; Anang Endaryanto; Ingrid Suryanti Surono; Mohamad Amin; Cita Rosita Sigit Prakoeswa

doi:10.15562/bmj.v11i1.3025

Bioinformatics assessment on the potential of Lipoteichoic Acid (LTA) of Lactic Acid Bacteria (LAB) as topical therapy for inflammatory skin diseases

Radityastuti ,

Anang Endaryanto ,

Ingrid Suryanti Surono ,

Mohamad Amin ,

Cita Rosita Sigit Prakoeswa ,

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DOI: https://doi.org/10.15562/bmj.v11i1.3025 |
Published: 2022-03-28

Abstract

Background: Lipoteichoic Acid (LTA) of Lactic Acid Bacteria (LAB) is a cell wall component of LAB with immunomodulatory properties towards inflammatory skin diseases due to immune dysregulation, such as atopic dermatitis. Topical glucocorticoids are given as the mainstay therapy in some inflammatory skin diseases. However, the adverse effects of topical glucocorticoids are commonly found, such as skin atrophy, reduced pigmentation, and masking-effect of skin infections. Therefore, safer therapy for inflammatory skin diseases is necessary to be developed. This study aimed to analyze the potential of LTA of LAB against glucocorticoid receptors (GR) as a topical therapy for inflammatory skin diseases.

Methods: This study used the bioinformatics method with molecular docking to predict the binding site and the binding affinity of LTA of LAB to GR and analyze the toxicity of LTA of LAB. According to the previous studies, this research was also conducted within silico/ molecular docking. Data were analyzed using Toxtree v 2. 6.13 application to evaluate the skin irritation.

Results: LAB’s lipoteichoic acid (LTA) has the same binding site with glucocorticoid control compounds to the GR. Toxicity analysis shows that LTA of LAB is not irritative to the skin.

Conclusion: LAB’s lipoteichoic acid (LTA) is potential as alternative topical therapy for inflammatory skin diseases.

References

Lew LC, Liong MT. Bioactives from probiotics for dermal health: functions and benefits. J Appl Microbiol. 2013;114(5):1241-1253.
Chapot-Chartier MP, Kulakauskas S. Cell wall structure and function in lactic acid bacteria. Microb Cell Fact. 2014;13 Suppl 1(Suppl 1):S9.
Dissanayake E, Shimojo N. Probiotics and Prebiotics in the Prevention and Treatment of Atopic Dermatitis. Pediatric Allergy, Immunology, and Pulmonology. 2016;29(4):174-80.
Tan PL, Liong MT. In silico approaches for probiotic-derived bioactives. Trends Biotechnol. 2014;32(12):599-601.
Schafer-Korting M, Kleuser B, Ahmed M, Holtje HD, Korting HC. Glucocorticoids for human skin: new aspects of the mechanism of action. Skin Pharmacol Physiol. 2005;18(3):103-114.
Bigas J, Sevilla LM, Carceller E, Boix J, Pérez P. Epidermal glucocorticoid and mineralocorticoid receptors act cooperatively to regulate epidermal development and counteract skin inflammation. Cell Death Dis. 2018;9(6):588.
Kubin ME, Hägg PM, Kokkonen N, Väyrynen JP, Haapasaari KM, Moilanen J, et al. Glucocorticoid receptors GRα and GRβ are expressed in inflammatory dermatoses. Eur J Dermatol. 2016;26(1):21-27.
Barnes PJ. Glucocorticosteroids. Handb Exp Pharmacol. 2017;237:93-115.
Simpson EL Md McR, Irvine AD Md, Eichenfield LF Md, Friedlander SF Md. Update on Epidemiology, Diagnosis, and Disease Course of Atopic Dermatitis. Semin Cutan Med Surg. 2016;35(5 Suppl):S84-S88.
Khaerunnisa S, Suhartati RA. Penelitian in silico untuk pemula. Surabaya: Airlangga University Press. 2020.
Kim H, Jung BJ, Jung JH, Kim JY, Chung SK, Chung DK. Lactobacillus plantarum lipoteichoic acid alleviates TNF-α-induced inflammation in the HT-29 intestinal epithelial cell line. Mol Cells. 2012;33(5):479-486.
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, et al. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J Immunol. 2008;180(4):2553-2561.
Lebeer S, Claes IJ, Vanderleyden J. Anti-inflammatory potential of probiotics: lipoteichoic acid makes a difference. Trends Microbiol. 2012;20(1):5-10.
Damayanti, Prakoeswa CRS, Purwanto DA, Endaryanto A, Siswandono. Molecular docking, pharmacokinetics, and toxicity prediction of Epigallocatechin-3-Gallate (EGCG) on IKK Receptor in photoaging prevention. Indian Journal of Forensic Medicine & Toxicology. 2020;14(2):1467-1473.
Amin M, Putra KS, Amin IF, Earlia N, Maulina D, Lukiati B, et al. Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening. Biology, Medicine, & Natural Product Chemistry. 2018;7(1):27-31.
Maulina D, Amin M, Lestari SR, Aziz M. Alanine as natural biopesticide from Mirabilis jalapa and its interaction with glutamate as an inhibitor in insects immune system. Berkala Penelitian Hayati. 2018;23(2):77-83.
Pangastuti A, Amin IF, Amin AZ, Amin M. Natural bioactive compound from Moringa oleifera against cancer based on in silico screening. Jurnal Teknologi. 2016;78(5):315-318.
Shiraishi T, Yokota S, Fukiya S, Yokota A. Structural diversity and biological significance of lipoteichoic acid in Gram-positive bacteria: focusing on beneficial probiotic lactic acid bacteria. Biosci Microbiota Food Health. 2016;35(4):147-161.
Kim KW, Kang SS, Woo SJ, Park OJ, Ahn KB, Song KD, et al. Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells. Front Microbiol. 2017;8:1827.
Stank A, Kokh DB, Fuller JC, Wade RC. Protein Binding Pocket Dynamics. Acc Chem Res. 2016;49(5):809-815.
Legiawati L, Fadilah F, Bramono K, Indriatmi W. In silico study of Centella asiatica active compounds as anti-inflammatory agent by decreasing Il-1 and Il-6 activity, promoting Il-4 activity. Journal of Pharmaceutical Sciences and Research. 2018;10(9):2142-2147.
Verheyen GR, Braeken E, Van Deun K, Van Miert S. Evaluation of in silico tools to predict the skin sensitization potential of chemicals. SAR QSAR Environ Res. 2017;28(1):59-73.
Roudsari MR, Karimi R, Sohrabvandi S, Mortazavian AM. Health effects of probiotics on the skin. Crit Rev Food Sci Nutr. 2015;55(9):1219-1240.
Knackstedt R, Knackstedt T, Gatherwright J. The role of topical probiotics in skin conditions: A systematic review of animal and human studies and implications for future therapies. Exp Dermatol. 2020;29(1):15-21.

[1] Lew LC, Liong MT. Bioactives from probiotics for dermal health: functions and benefits. J Appl Microbiol. 2013;114(5):1241-1253.

[2] Chapot-Chartier MP, Kulakauskas S. Cell wall structure and function in lactic acid bacteria. Microb Cell Fact. 2014;13 Suppl 1(Suppl 1):S9.

[3] Dissanayake E, Shimojo N. Probiotics and Prebiotics in the Prevention and Treatment of Atopic Dermatitis. Pediatric Allergy, Immunology, and Pulmonology. 2016;29(4):174-80.

[4] Tan PL, Liong MT. In silico approaches for probiotic-derived bioactives. Trends Biotechnol. 2014;32(12):599-601.

[5] Schafer-Korting M, Kleuser B, Ahmed M, Holtje HD, Korting HC. Glucocorticoids for human skin: new aspects of the mechanism of action. Skin Pharmacol Physiol. 2005;18(3):103-114.

[6] Bigas J, Sevilla LM, Carceller E, Boix J, Pérez P. Epidermal glucocorticoid and mineralocorticoid receptors act cooperatively to regulate epidermal development and counteract skin inflammation. Cell Death Dis. 2018;9(6):588.

[7] Kubin ME, Hägg PM, Kokkonen N, Väyrynen JP, Haapasaari KM, Moilanen J, et al. Glucocorticoid receptors GRα and GRβ are expressed in inflammatory dermatoses. Eur J Dermatol. 2016;26(1):21-27.

[8] Barnes PJ. Glucocorticosteroids. Handb Exp Pharmacol. 2017;237:93-115.

[9] Simpson EL Md McR, Irvine AD Md, Eichenfield LF Md, Friedlander SF Md. Update on Epidemiology, Diagnosis, and Disease Course of Atopic Dermatitis. Semin Cutan Med Surg. 2016;35(5 Suppl):S84-S88.

[10] Khaerunnisa S, Suhartati RA. Penelitian in silico untuk pemula. Surabaya: Airlangga University Press. 2020.

[11] Kim H, Jung BJ, Jung JH, Kim JY, Chung SK, Chung DK. Lactobacillus plantarum lipoteichoic acid alleviates TNF-α-induced inflammation in the HT-29 intestinal epithelial cell line. Mol Cells. 2012;33(5):479-486.

[12] Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, et al. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J Immunol. 2008;180(4):2553-2561.

[13] Lebeer S, Claes IJ, Vanderleyden J. Anti-inflammatory potential of probiotics: lipoteichoic acid makes a difference. Trends Microbiol. 2012;20(1):5-10.

[14] Damayanti, Prakoeswa CRS, Purwanto DA, Endaryanto A, Siswandono. Molecular docking, pharmacokinetics, and toxicity prediction of Epigallocatechin-3-Gallate (EGCG) on IKK Receptor in photoaging prevention. Indian Journal of Forensic Medicine & Toxicology. 2020;14(2):1467-1473.

[15] Amin M, Putra KS, Amin IF, Earlia N, Maulina D, Lukiati B, et al. Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening. Biology, Medicine, & Natural Product Chemistry. 2018;7(1):27-31.

[16] Maulina D, Amin M, Lestari SR, Aziz M. Alanine as natural biopesticide from Mirabilis jalapa and its interaction with glutamate as an inhibitor in insects immune system. Berkala Penelitian Hayati. 2018;23(2):77-83.

[17] Pangastuti A, Amin IF, Amin AZ, Amin M. Natural bioactive compound from Moringa oleifera against cancer based on in silico screening. Jurnal Teknologi. 2016;78(5):315-318.

[18] Shiraishi T, Yokota S, Fukiya S, Yokota A. Structural diversity and biological significance of lipoteichoic acid in Gram-positive bacteria: focusing on beneficial probiotic lactic acid bacteria. Biosci Microbiota Food Health. 2016;35(4):147-161.

[19] Kim KW, Kang SS, Woo SJ, Park OJ, Ahn KB, Song KD, et al. Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells. Front Microbiol. 2017;8:1827.

[20] Stank A, Kokh DB, Fuller JC, Wade RC. Protein Binding Pocket Dynamics. Acc Chem Res. 2016;49(5):809-815.

[21] Legiawati L, Fadilah F, Bramono K, Indriatmi W. In silico study of Centella asiatica active compounds as anti-inflammatory agent by decreasing Il-1 and Il-6 activity, promoting Il-4 activity. Journal of Pharmaceutical Sciences and Research. 2018;10(9):2142-2147.

[22] Verheyen GR, Braeken E, Van Deun K, Van Miert S. Evaluation of in silico tools to predict the skin sensitization potential of chemicals. SAR QSAR Environ Res. 2017;28(1):59-73.

[23] Roudsari MR, Karimi R, Sohrabvandi S, Mortazavian AM. Health effects of probiotics on the skin. Crit Rev Food Sci Nutr. 2015;55(9):1219-1240.

[24] Knackstedt R, Knackstedt T, Gatherwright J. The role of topical probiotics in skin conditions: A systematic review of animal and human studies and implications for future therapies. Exp Dermatol. 2020;29(1):15-21.

Bioinformatics assessment on the potential of Lipoteichoic Acid (LTA) of Lactic Acid Bacteria (LAB) as topical therapy for inflammatory skin diseases

Abstract

References

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