Kefir Gel  Increased The Epithelial Thickness in First-Degree Sunburn

Titik Kusumawinakhyu; Edi Dharmana; Taufiqurrachman Nasihun; Chodidjah Chodidjah; Dwi Pudjonarko

doi:10.15562/bmj.v10i3.3017

Kefir Gel Increased The Epithelial Thickness in First-Degree Sunburn

Titik Kusumawinakhyu ,

Edi Dharmana ,

Taufiqurrachman Nasihun ,

Chodidjah Chodidjah ,

Dwi Pudjonarko ,

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DOI: https://doi.org/10.15562/bmj.v10i3.3017 |
Published: 2021-12-30

Abstract

Background: UVB radiation may cause skin to experience acute symptoms such as erythema and pain. The acute symptoms are categorized as first-degree sunburn. Prevention can be done through kefir gel administration.

Purpose: This study aimed to determine differences between effect kefir oral, topical gel, and combination (oral and topical gel) effects and the negative control group on epithelial thickness in first-degree sunburn.

Method: An experimental study with post-test only control group design was conducted on BALB/c mice, and the negative control group exposed only with UVB without kefir and oral, topical, and combination (oral and gel) group administration with dose of 0.25 mL/KgBB/day. The epithelial thickness was determined by histopathological preparations of back skin tissue through HE painting. Distribution analysis of data normality was through Shapiro-Wilk test. Homogeneity test of both groups was done through Levene test. One-way Anova test was continued with Post Hoc TukeyHSD test for epithelial thickness.

Result: The analysis of Post Hoc TukeyHSD test showed p score <0.05 which meant that there was a significantly higher difference in epithelial thickness of topical group (12.59 ± 2.067) than the negative control (5.30 ± 0.353). Kefir gel dose of 0.25mL/kgBW/day escalates thickness of epithelialization compared to negative control group.

Conclusion: There is a significant difference in epithelial thickness between the negative control group and the topical kefir gel treatment groups and the combination of both (oral and topical).

References

Guerra KC, Urban K, Crane JS. Sunburn. StatPearls [Internet]. 2021 Aug 4.
Ardhie AM. Radikal bebas dan peran antioksidan dalam mencegah penuaan. Medicinus. 2011;24(1):4-9.
Wilson BD, Moon S, Armstrong F. Comprehensive review of ultraviolet radiation and the current status on sunscreens. The Journal of clinical and aesthetic dermatology. 2012 Sep;5(9):18.
González Maglio DH, Paz ML, Leoni J. Sunlight effects on immune system: is there something else in addition to UV-induced immunosuppression?. BioMed research international. 2016.
Timothy J.K and Luisa AD. Inflammation and Wound Healing: The Rule Of Macrophage. 2013;1-14
Febram B, Wientarsih I, Pontjo B. Activity of ambon banana (Musa paradisiaca var. sapientum) stem extract in ointment formulation on the wound healing process of mice skin (Mus musculus albinus). Majalah Obat Tradisional. 2010;15(3):121-37.
Siahaan MS, Pangkahila W, Aman IG. Gel ekstrak daun meniran (Phyllanthus niruri) meningkatkan epitelisasi penyembuhan luka pada kulit tikus putih jantan galur Wistar (Rattus norvegicus). Jurnal Biomedik: JBM. 2017;9(1).
Chen YP, Chen MJ. Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice. PloS one. 2013 Nov 11;8(11):e78789.
Prasetyo AT, Heridadi E. The application of moist exposed burn ointment (MEBO) and binahong leaves in treating partial thickness burn: A case report. Jurnal Plastik Rekonstruksi. 2013;2(4).
Yunanda V, Rinanda T. Aktivitas penyembuhan luka sediaan topikal ekstrak Bawang Merah (Allium cepa) terhadap luka sayat kulit Mencit (Mus musculus). J. Vet. Desember. 2016;17:606-14.
Yanhendri SW. Berbagai bentuk sediaan topikal dalam dermatologi. Cermin Dunia Kedokteran. 2012;194(39):6.
Makiyah A, Husin UA, Sadeli R. Efek imunostimulasi ekstrak etanol umbi iles-iles terhadap aktivitas fagositosis sel makrofag pada tikus putih strain wistar yang diinokulasi staphylococcus aureus. MKB. 2016 Jun 30;48(2):68-77.
Patel S. Emerging trends in nutraceutical applications of whey protein and its derivatives. Journal of food science and technology. 2015 Nov;52(11):6847-58.

[1] Guerra KC, Urban K, Crane JS. Sunburn. StatPearls [Internet]. 2021 Aug 4.

[2] Ardhie AM. Radikal bebas dan peran antioksidan dalam mencegah penuaan. Medicinus. 2011;24(1):4-9.

[3] Wilson BD, Moon S, Armstrong F. Comprehensive review of ultraviolet radiation and the current status on sunscreens. The Journal of clinical and aesthetic dermatology. 2012 Sep;5(9):18.

[4] González Maglio DH, Paz ML, Leoni J. Sunlight effects on immune system: is there something else in addition to UV-induced immunosuppression?. BioMed research international. 2016.

[5] Timothy J.K and Luisa AD. Inflammation and Wound Healing: The Rule Of Macrophage. 2013;1-14

[6] Febram B, Wientarsih I, Pontjo B. Activity of ambon banana (Musa paradisiaca var. sapientum) stem extract in ointment formulation on the wound healing process of mice skin (Mus musculus albinus). Majalah Obat Tradisional. 2010;15(3):121-37.

[7] Siahaan MS, Pangkahila W, Aman IG. Gel ekstrak daun meniran (Phyllanthus niruri) meningkatkan epitelisasi penyembuhan luka pada kulit tikus putih jantan galur Wistar (Rattus norvegicus). Jurnal Biomedik: JBM. 2017;9(1).

[8] Chen YP, Chen MJ. Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice. PloS one. 2013 Nov 11;8(11):e78789.

[9] Prasetyo AT, Heridadi E. The application of moist exposed burn ointment (MEBO) and binahong leaves in treating partial thickness burn: A case report. Jurnal Plastik Rekonstruksi. 2013;2(4).

[10] Yunanda V, Rinanda T. Aktivitas penyembuhan luka sediaan topikal ekstrak Bawang Merah (Allium cepa) terhadap luka sayat kulit Mencit (Mus musculus). J. Vet. Desember. 2016;17:606-14.

[11] Yanhendri SW. Berbagai bentuk sediaan topikal dalam dermatologi. Cermin Dunia Kedokteran. 2012;194(39):6.

[12] Makiyah A, Husin UA, Sadeli R. Efek imunostimulasi ekstrak etanol umbi iles-iles terhadap aktivitas fagositosis sel makrofag pada tikus putih strain wistar yang diinokulasi staphylococcus aureus. MKB. 2016 Jun 30;48(2):68-77.

[13] Patel S. Emerging trends in nutraceutical applications of whey protein and its derivatives. Journal of food science and technology. 2015 Nov;52(11):6847-58.

Kefir Gel Increased The Epithelial Thickness in First-Degree Sunburn

Abstract

References

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