Antioxidants and immunomodulatory effect of black cumin seed oil in at-risk metabolic syndrome

Akrom Akrom; Endang Darmawan; Nuril Maulida; Syuhada Syuhada

doi:10.15562/bmj.v10i3.2822

Antioxidants and immunomodulatory effect of black cumin seed oil in at-risk metabolic syndrome

Akrom Akrom ,

Endang Darmawan ,

Nuril Maulida ,

Syuhada Syuhada ,

PDF |
DOI: https://doi.org/10.15562/bmj.v10i3.2822 |
Published: 2021-12-28

Abstract

Introduction: Black Cumin Seed Oil (BCSO) is a traditional medicine widely used as an antioxidant and immunomodulator. Objective: The purpose of this study is to investigate the effect of dose variation of BCSO (1.5 ml/day versus 3 ml/day) on IL-10 activity and MDA levels in patients at risk of MS at Jetis 1 Public Health Center (J1PHC), Bantul Regency, Yogyakarta, Indonesia.

Methods: We conducted an analytical cross-sectional study. 66 patients at risk of metabolic syndrome (MS) were divided into two groups. In stage 1, group 1 received a 1.5 ml/day dose, and group 2 received 3 ml/day for 20 days. The clinical parameters of MS, IL-10 activity, and MDA levels were measured at the end of the study stages.

Results: The mean activity of IL-10 in the 1.5 ml/day dose group was 4.83 and in the 3 ml/day dose group was 5.49, which showed an increase of around 13.66% (p = 0.300). The mean MDA level in the 1.5 ml/day group was 3.92 ?mol/L, which increased to 4.31 ?mol/L in the 3 ml/day group or approximately 9.95% (p = 0.802). Statistically, IL-10 activity and MDA levels in the two-dose groups did not differ significantly.

Conclusion: The results of this study indicate that the administration of BCSO at doses of 1.5 ml/day and 3 ml/day for 20 days has the same effect on IL-10 activity and MDA levels in patients at risk of MS.

References

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Shabalala SC, Dludla P V., Mabasa L, Kappo AP, Basson AK, Pheiffer C, et al. The effect of adiponectin in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) and the potential role of polyphenols in the modulation of adiponectin signaling. Biomed Pharmacother [Internet]. 2020;131(September):110785. Available from: https://doi.org/10.1016/j.biopha.2020.110785
Strzelak A, Ratajczak A, Adamiec A, Feleszko W. Tobacco smoke induces and alters immune responses in the lung triggering inflammation, allergy, asthma and other lung diseases: A mechanistic review. Int J Environ Res Public Health. 2018;15(5).
Kelley N, Jeltema D, Duan Y, He Y. The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. Int J Mol Sci. 2019;20(13):1–24.
Celik I, Temur A, Isik I. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum) flowers infusion against trichloroacetic acid-exposed in rats. Food Chem Toxicol [Internet]. 2009;47(1):145–9. Available from: http://dx.doi.org/10.1016/j.fct.2008.10.020
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Wang S-H, Wang Y-Z, Zhang K-Y, Shen J-H, Zhou H-Q, Qiu ELSEVIER X-Y, et al. Effect of superoxide dismutase and malondialdehyde metabolic changes on carcinogenesis of gastric carcinoma. World J Gastroenterol [Internet]. 2005;11(28):4305–10. Available from: www.wjgnet.comhttp://www.wjgnet.com/1007-9327/11/4305.asp
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Hidayati T, Akrom, Indrayanti, Sagiran. Chemopreventive effect of black cumin seed oil (BCSO) by increasing p53 expression in dimethylbenzanthracene (DMBA)-induced Sprague Dawley rats. Res J Chem Environ. 2019;23(8).
Arjumand S, Shahzad M, Shabbir A, Yousaf MZ. Thymoquinone attenuates rheumatoid arthritis by downregulating TLR2, TLR4, TNF-? IL-1, and NF?B expression levels. Biomed Pharmacother [Internet]. 2019;111(October 2018):958–63. Available from: https://doi.org/10.1016/j.biopha.2019.01.006
Heshmati J, Namazi N. Effects of black seed (Nigella sativa) on metabolic parameters in diabetes mellitus: A systematic review. Vol. 23, Complementary Therapies in Medicine. 2015.
Agustiarini V, Darmawan E. The effects of dosage variation in black cumin seed oil ( Nigella sativa L .) use on HbA1c levels and interleukin-17A expression in patients at risks of metabolic syndrome. 2019;9(2):261–70.
Fajar DR, Akrom, Darmawan E. The influence of black cumin seed oil therapy with dosage of 1.5 mL/day and 3 mL/day to interleukin-21 (IL-21) expression of the patients with metabolic syndrome risk. In: IOP Conference Series: Materials Science and Engineering. 2017.
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Aulia Rahim A and ED. HbA1c and interleukin-17a level inpatient at risk for metabolic syndrome in Yogyakarta. Res J Chem Environ [Internet]. 2019;23(9):38–42. Available from: https://worldresearchersassociations.com/Archives/RJCE/Vol(23)2019/September2019.aspx
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Llamas-Velasco M, Ovejero-Merino E, Salgado-Boquete L. Obesity — A Risk Factor for Psoriasis and COVID-19. Actas Dermo-Sifiliográficas (English Ed [Internet]. 2021;(xxxx). Available from: http://dx.doi.org/10.1016/j.adengl.2021.03.013
Becerra-Díaz M, Wills-Karp M, Heller NM. New perspectives on the regulation of type II inflammation in asthma. Vol. 6, F1000Research. Faculty of 1000 Ltd; 2017.
Hamdan A, Idrus RH, Mokhtar MH. Effects of nigella sativa on type-2 diabetes mellitus: A systematic review. Vol. 16, International Journal of Environmental Research and Public Health. 2019.
Akrom, Mustofa. Black cumin seed oil increases phagocytic activity and secretion of IL-12 by macrophages. Biomed Res. 2017;28(12).
Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. International Immunopharmacology. 2005.
Hidayati T, Gugun AM. Exercise Habits , Hematogram and Blood Chemistry of Diabetes Mellitus Patients at Risk For Metabolic Syndrome , Bantul Public Health Center. 2020;24(02):475–87.
Herlina, Aziz SA, Kurniawati A, Faridah DN. Changes of Thymoquinone, Thymol, and Malondialdehyde Content of Black Cumin (Nigella sativa L.) in Response to Indonesia Tropical Altitude Variation. HAYATI J Biosci. 2017;24(3):156–61.
Jin P, Cong S. LOX-1 and atherosclerotic-related diseases. Vol. 491, Clinica Chimica Acta. Elsevier B.V.; 2019. p. 24–9.
Hidayati T, Pramono A, Jenie IM, Soesatyo MH. Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA inducedrats. Biomed Res. 2017;28(4):1755–60.
Kulyar MF e. A, Li R, Mehmood K, Waqas M, Li K, Li J. Potential influence of Nagella sativa (Black cumin) in reinforcing immune system: A hope to decelerate the COVID-19 pandemic. Phytomedicine [Internet]. 2020;(April):153277. Available from: https://doi.org/10.1016/j.phymed.2020.153277
Hasanpour M, Iranshahy M, Iranshahi M. The application of metabolomics in investigating anti-diabetic activity of medicinal plants. Biomed Pharmacother [Internet]. 2020;128(March):110263. Available from: https://doi.org/10.1016/j.biopha.2020.110263
Akrom A, Nurfadjrin R, Darmawan E, Hidayati T. Black Cumin Seed Oil Antidiabetogenic by Increasing Pancreatic P53 Expression. Int J Public Heal Sci. 2018;7(3):207.
Sarwar R, Pierce N, Koppe S. Obesity and nonalcoholic fatty liver disease: Current perspectives. Diabetes, Metab Syndr Obes Targets Ther. 2018;11:533–42.

[1] Almerie MQ, Kerrigan DD. The association between obesity and poor outcome after COVID-19 indicates a potential therapeutic role for montelukast. Med Hypotheses [Internet]. 2020;143(May):109883. Available from: https://doi.org/10.1016/j.mehy.2020.109883

[2] van Koppen A, Verschuren L, van den Hoek AM, Verheij J, Morrison MC, Li K, et al. Uncovering a Predictive Molecular Signature for the Onset of NASH-Related Fibrosis in a Translational NASH Mouse Model. Cmgh [Internet]. 2018;5(1):83-98.e10. Available from: https://doi.org/10.1016/j.jcmgh.2017.10.001

[3] Huang I, Lim MA, Pranata R. Diabetes mellitus is associated with increased mortality and severity of disease in COVID-19 pneumonia – A systematic review, meta-analysis, and meta-regression: Diabetes and COVID-19. Diabetes Metab Syndr Clin Res Rev [Internet]. 2020;14(4):395–403. Available from: https://doi.org/10.1016/j.dsx.2020.04.018

[4] Shabalala SC, Dludla P V., Mabasa L, Kappo AP, Basson AK, Pheiffer C, et al. The effect of adiponectin in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) and the potential role of polyphenols in the modulation of adiponectin signaling. Biomed Pharmacother [Internet]. 2020;131(September):110785. Available from: https://doi.org/10.1016/j.biopha.2020.110785

[5] Strzelak A, Ratajczak A, Adamiec A, Feleszko W. Tobacco smoke induces and alters immune responses in the lung triggering inflammation, allergy, asthma and other lung diseases: A mechanistic review. Int J Environ Res Public Health. 2018;15(5).

[6] Kelley N, Jeltema D, Duan Y, He Y. The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. Int J Mol Sci. 2019;20(13):1–24.

[7] Celik I, Temur A, Isik I. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum) flowers infusion against trichloroacetic acid-exposed in rats. Food Chem Toxicol [Internet]. 2009;47(1):145–9. Available from: http://dx.doi.org/10.1016/j.fct.2008.10.020

[8] Moorthy B, Chu C, Carlin DJ. Polycyclic aromatic hydrocarbons: From metabolism to lung cancer. Vol. 145, Toxicological Sciences. Oxford University Press; 2015. p. 5–15.

[9] Wang S-H, Wang Y-Z, Zhang K-Y, Shen J-H, Zhou H-Q, Qiu ELSEVIER X-Y, et al. Effect of superoxide dismutase and malondialdehyde metabolic changes on carcinogenesis of gastric carcinoma. World J Gastroenterol [Internet]. 2005;11(28):4305–10. Available from: www.wjgnet.comhttp://www.wjgnet.com/1007-9327/11/4305.asp

[10] Hadi S, Mirmiran P, Daryabeygi-Khotbesara R, Hadi V. Effect of Nigella sativa oil extract on inflammatory cytokine response and oxidative stress among people with type 2 diabetes mellitus: A randomized, double-blind, placebo controlled trial. Prog Nutr. 2018;20(9):127–33.

[11] Hidayati T, Akrom, Indrayanti, Sagiran. Chemopreventive effect of black cumin seed oil (BCSO) by increasing p53 expression in dimethylbenzanthracene (DMBA)-induced Sprague Dawley rats. Res J Chem Environ. 2019;23(8).

[12] Arjumand S, Shahzad M, Shabbir A, Yousaf MZ. Thymoquinone attenuates rheumatoid arthritis by downregulating TLR2, TLR4, TNF-? IL-1, and NF?B expression levels. Biomed Pharmacother [Internet]. 2019;111(October 2018):958–63. Available from: https://doi.org/10.1016/j.biopha.2019.01.006

[13] Heshmati J, Namazi N. Effects of black seed (Nigella sativa) on metabolic parameters in diabetes mellitus: A systematic review. Vol. 23, Complementary Therapies in Medicine. 2015.

[14] Agustiarini V, Darmawan E. The effects of dosage variation in black cumin seed oil ( Nigella sativa L .) use on HbA1c levels and interleukin-17A expression in patients at risks of metabolic syndrome. 2019;9(2):261–70.

[15] Fajar DR, Akrom, Darmawan E. The influence of black cumin seed oil therapy with dosage of 1.5 mL/day and 3 mL/day to interleukin-21 (IL-21) expression of the patients with metabolic syndrome risk. In: IOP Conference Series: Materials Science and Engineering. 2017.

[16] Ray S, Fitzpatrick S, Golubic R, Fisher S. Oxford Handbook of Clinical and Healthcare Research. Sources bias. 2016;608.

[17] Aulia Rahim A and ED. HbA1c and interleukin-17a level inpatient at risk for metabolic syndrome in Yogyakarta. Res J Chem Environ [Internet]. 2019;23(9):38–42. Available from: https://worldresearchersassociations.com/Archives/RJCE/Vol(23)2019/September2019.aspx

[18] McCracken E, Monaghan M, Sreenivasan S. Pathophysiology of the metabolic syndrome. Clin Dermatol [Internet]. 2018;36(1):14–20. Available from: http://dx.doi.org/10.1016/j.clindermatol.2017.09.004

[19] Llamas-Velasco M, Ovejero-Merino E, Salgado-Boquete L. Obesity — A Risk Factor for Psoriasis and COVID-19. Actas Dermo-Sifiliográficas (English Ed [Internet]. 2021;(xxxx). Available from: http://dx.doi.org/10.1016/j.adengl.2021.03.013

[20] Becerra-Díaz M, Wills-Karp M, Heller NM. New perspectives on the regulation of type II inflammation in asthma. Vol. 6, F1000Research. Faculty of 1000 Ltd; 2017.

[21] Hamdan A, Idrus RH, Mokhtar MH. Effects of nigella sativa on type-2 diabetes mellitus: A systematic review. Vol. 16, International Journal of Environmental Research and Public Health. 2019.

[22] Akrom, Mustofa. Black cumin seed oil increases phagocytic activity and secretion of IL-12 by macrophages. Biomed Res. 2017;28(12).

[23] Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. International Immunopharmacology. 2005.

[24] Hidayati T, Gugun AM. Exercise Habits , Hematogram and Blood Chemistry of Diabetes Mellitus Patients at Risk For Metabolic Syndrome , Bantul Public Health Center. 2020;24(02):475–87.

[25] Herlina, Aziz SA, Kurniawati A, Faridah DN. Changes of Thymoquinone, Thymol, and Malondialdehyde Content of Black Cumin (Nigella sativa L.) in Response to Indonesia Tropical Altitude Variation. HAYATI J Biosci. 2017;24(3):156–61.

[26] Jin P, Cong S. LOX-1 and atherosclerotic-related diseases. Vol. 491, Clinica Chimica Acta. Elsevier B.V.; 2019. p. 24–9.

[27] Hidayati T, Pramono A, Jenie IM, Soesatyo MH. Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA inducedrats. Biomed Res. 2017;28(4):1755–60.

[28] Kulyar MF e. A, Li R, Mehmood K, Waqas M, Li K, Li J. Potential influence of Nagella sativa (Black cumin) in reinforcing immune system: A hope to decelerate the COVID-19 pandemic. Phytomedicine [Internet]. 2020;(April):153277. Available from: https://doi.org/10.1016/j.phymed.2020.153277

[29] Hasanpour M, Iranshahy M, Iranshahi M. The application of metabolomics in investigating anti-diabetic activity of medicinal plants. Biomed Pharmacother [Internet]. 2020;128(March):110263. Available from: https://doi.org/10.1016/j.biopha.2020.110263

[30] Akrom A, Nurfadjrin R, Darmawan E, Hidayati T. Black Cumin Seed Oil Antidiabetogenic by Increasing Pancreatic P53 Expression. Int J Public Heal Sci. 2018;7(3):207.

[31] Sarwar R, Pierce N, Koppe S. Obesity and nonalcoholic fatty liver disease: Current perspectives. Diabetes, Metab Syndr Obes Targets Ther. 2018;11:533–42.

Antioxidants and immunomodulatory effect of black cumin seed oil in at-risk metabolic syndrome

Abstract

References

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