Ethyl p-methoxycinnamate isolated from Kaempferia galanga L. rhizome reduces airway remodeling in asthmatic rat models

Ismiranti Andarini; Harsono Salimo; Bambang Purwanto; Setyo Sri Rahardjo; Brian Wasita; Vitri Widyaningsih

doi:10.15562/bmj.v10i3.2701

Ethyl p-methoxycinnamate isolated from Kaempferia galanga L. rhizome reduces airway remodeling in asthmatic rat models

Ismiranti Andarini ,

Harsono Salimo ,

Bambang Purwanto ,

Setyo Sri Rahardjo ,

Brian Wasita ,

Vitri Widyaningsih ,

PDF |
DOI: https://doi.org/10.15562/bmj.v10i3.2701 |
Published: 2021-12-30

Abstract

Background: Asthma causes changes in the airway structure called airway remodeling due to chronic inflammatory reactions. Transforming Growth Factor-? (TGF-?) is one of the inflammatory mediators which has a significant role in the remodeling and inflammatory process. One of the medicinal plants known to have anti-inflammatory potential is Kaempferia galanga L. This study aims to analyze the effect of ethyl p-methoxycinnamate isolated from Kaempferia galanga L. rhizome on the expression of TGF-? and fibrosis in the bronchial mucosa of asthmatic rat models.

Methods: We conducted an experimental laboratory study using the posttest only control group design. Thirty-six Wistar male white rats were allocated into six groups, NC (negative control), PC (positive control), T1 (treatment group), T2 (treatment group), T3 (treatment group), and T4 (treatment group) receiving 1% ovalbumin in alum (OVA); 1% OVA as well as 1 mg/kg steroid; 1% OVA, 1 mg/kg steroid, and 200 mg/kg ethyl p-methoxycinnamate isolated from Kaempferia galanga L. rhizome (KG-EPMC); 1% OVA, 1 mg/kg steroid, and 400 mg/kg KG-EPMC; 1% OVA and 200 mg/kg KG-EPMC; and 1% OVA as well as 400 mg/kg KG-EPMC, respectively. Data were analyzed with SPSS version 22.0 for Windows.

Results: The expressions of TGF-? and fibrosis were significantly lower in positive control as well as treatment groups than those of the negative control group (only receiving OVA) (p<0.05). There were no significant differences between positive control and treatment groups regarding TGF-? level and fibrosis (p>0.005).

Conclusion: Ethyl p-methoxycinnamate isolated from Kaempferia galanga L. rhizome exhibits antiasthma effect by reducing the expression of TGF-? and fibrosis in the bronchial mucosa of an asthmatic rat model. Hence, it can be a potential therapy for asthma.

References

1. Holgate ST, Wenzel S, Postma DS, Weiss ST, Renz H, Sly PD. Asthma. Nat Rev Dis Primers. 2015;1(1):15025.
2. Fehrenbach H, Wagner C, Wegmann M. Airway remodeling in asthma: what really matters. Cell Tissue Res. 2017;367(3):551–69.
3. Aghasafari P, George U, Pidaparti R. A review of inflammatory mechanism in airway diseases. Inflamm Res. 2019;68(1):59–74.
4. Tian X, Tian X, Huo R, Chang Q, Zheng G, Du Y, et al. Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-? 1 induced epithelial–mesenchymal transition. Hum Vaccin Immunother. 2017;13(8):1758–1764.
5. Wnuk D, Paw M, Ryczek K, Bochenek G, S?adek K, Madeja Z, et al. Enhanced asthma-related fibroblast to myofibroblast transition is the result of profibrotic TGF-?/Smad2/3 pathway intensification and antifibrotic TGF-?/Smad1/5/(8)9 pathway impairment. Sci Rep. 2020;10(1):16492.
6. Fang P, Xue Y, Zhang Y, Fan N, Ou L, Leng L, et al. SIRT7 regulates the TGF-?1-induced proliferation and migration of mouse airway smooth muscle cells by modulating the expression of TGF-? receptor I. Biomed Pharmacother. 2018;104:781-787.
7. Amaral-Machado L, Oliveira WN, Moreira-Oliveira SS, Pereira DT, Alencar ÉN, Tsapis N, et al. Use of Natural Products in Asthma Treatment. Evidence-based Complement Altern Med. 2020;2020:1–35.
8. Umar M, Asmawi M, Sadikun A, Majid A, Al-Suede F, Hassan L, et al. Ethyl-p-methoxycinnamate isolated from kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-?, and angiogenesis by blocking endothelial functions. Clinics (Sao Paulo). 2014;69(2):134–144.
9. McMillan SJ, Xanthou G, Lloyd CM. Therapeutic administration of Budesonide ameliorates allergen-induced airway remodelling. Clin Exp Allergy. 2005;35(3):388-396.
10. Fedchenko N, Reifenrath J. Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue – a review. Diagn Pathol. 2014;9(1):221.
11. Papi A, Brightling C, Pedersen SE, Reddel HK. Asthma. Lancet. 2018;391(10122):783–800.
12. Ish P, Malhotra N, Gupta N. GINA 2020: what's new and why?. J Asthma. 2021;58(10):1273-1277.
13. Ward CE, Baptist AP. Characteristics of Complementary and Alternative Medicine (CAM) use among older adults with asthma. J Asthma. 2016;53(5):546-552.
14. Kumar A. Phytochemistry, pharmacological activities and uses of traditional medicinal plant Kaempferia galanga L. - An overview. J Ethnopharmacol. 2020;253:112667.
15. Umar MI, Asmawi MZ, Sadikun A, Atangwho IJ, Yam MF, Altaf R, et al. Bioactivity-guided isolation of ethyl-p-methoxycinnamate, an anti-inflammatory constituent, from Kaempferia galanga L. extracts. Molecules. 2012;17(7):8720–8734.
16. Ge Y, Cheng R, Sun S, Zhang S, Li L, Jiang J, et al. Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor-?/Smad3 signaling pathway. Biomed Pharmacother. 2019;119:109429
17. Sachdeva K, Do DC, Zhang Y, Hu X, Chen J, Gao P. Environmental Exposures and Asthma Development: Autophagy, Mitophagy, and Cellular Senescence. Front Immunol. 2019;10:1–16.
18. Doherty T, Broide D. Cytokines and growth factors in airway remodeling in asthma. Curr Opin Immunol. 2007;19(6):676–680.
19. Tang X, Nian H, Li X, Yang Y, Wang X, Xu L, et al. Effects of the combined extracts of Herba Epimedii and Fructus Ligustrilucidi on airway remodeling in the asthmatic rats with the treatment of budesonide. BMC Complement Altern Med [Internet]. 2017;17(1):380.
20. Elia I, Schmieder R, Christen S, Fendt SM. Organ-Specific Cancer Metabolism and Its Potential for Therapy. Handb Exp Pharmacol. 2016;233:321-353.
21. Jaffer OA, Carter AB, Sanders PN, Dibbern ME, Winters CJ, Murthy S, et al. Mitochondrial-Targeted Antioxidant Therapy Decreases Transforming Growth Factor-?–Mediated Collagen Production in a Murine Asthma Model. Am J Respir Cell Mol Biol. 2015;52(1):106–115.
22. Qian J, Xu Y, Yu Z. Budesonide and Calcitriol Synergistically Inhibit Airway Remodeling in Asthmatic Mice. Can Respir J. 2018;2018:5259240.

Ethyl p-methoxycinnamate isolated from Kaempferia galanga L. rhizome reduces airway remodeling in asthmatic rat models

Abstract

References

How to Cite

Search Panel