Epidermal Growth Factor (EGF) representing the role of other growth factors contained in Platelet-Rich Plasma (PRP)

Dessyta Sukma Nurmaulinda; Agus Santoso Budi; Lobredia Zarasade; Budi Utomo

doi:10.15562/bmj.v10i2.2524

Epidermal Growth Factor (EGF) representing the role of other growth factors contained in Platelet-Rich Plasma (PRP)

Dessyta Sukma Nurmaulinda ,

Agus Santoso Budi ,

Lobredia Zarasade ,

Budi Utomo ,

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DOI: https://doi.org/10.15562/bmj.v10i2.2524 |
Published: 2021-08-21

Abstract

Background: Wound care with modern dressings that have been widely used takes time to achieve healing. Therefore, Epidermal Growth Factor (EGF) and Platelet-Rich Plasma (PRP) are developed in wound healing therapy. EGF as the primary growth factor examines whether it is sufficient to represent the role of other growth factors in PRP. EGF is also chosen because of its ease of provision and longer shelf life compared to PRP. This study evaluates the (EGF) representing the role of other growth factors contained in PRP.

Methods: The study was performed on 66 full-thickness wounds in 6 groups of 36 healthy male Oryctolagus cuniculus rabbits. Four treatment groups were given EGF and PRP therapies. Two control groups were given no treatment. Half of the groups were evaluated on the fifth day and the rest on the fourteenth day. Assessment on the clinical macroscopic and histopathological numbers of fibroblasts, capillary blood vessels, and type III collagen fibers were stained with Hematoxylin Eosin (HE) and Masson's Trichrome. Data were analyzed using SPSS version 23 for Windows.

Results: There was a statistically significant difference in fibroplasia (p=0.014; p=0.018) on the fifth and fourteenth days. However, there was no significant difference in angiogenesis (p=0.183; p=0.524) or collagenization (p=0.218; p=0.278) on the fifth and fourteenth day. On the fifth day, the number of capillary vessels was highest in the PRP groups (10.60±4.13), and the ratio of type III collagen fibers (53.00±13.00) was the highest in the EGF group. On the fourteenth day, the number of capillaries and the ratio of type III collagen fibers was the highest in the EGF groups (77.00±16.00).

Conclusion: EGF greatly increases the speed of macroscopic healing, accelerates fibroplasia, induces angiogenesis, and is also involved in collagen deposition compared to PRP administration, especially when compared to untreated wounds.

References

Obagi Z, Damiani G, Grada A, Falanga V. Principles of Wound Dressings: A Review. Surg Technol Int. 2019;35:50-57.
Pugliese E, Coentro JQ, Raghunath M, Zeugolis DI. Wound healing and scar wars. Adv Drug Deliv Rev. 2018;129:1-3.
Hardwicke J, Schmaljohann D, Boyce D, Thomas D. Epidermal growth factor therapy and wound healing--past, present and future perspectives. Surgeon. 2008;6(3):172-177.
Xu P, Wu Y, Zhou L, Yang Z, Zhang X, Hu X, et al. Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization. Burns Trauma. 2020 Aug 14;8:tkaa028.
Yamamoto A, Shimizu N, Kuroyanagi Y. Potential of wound dressing composed of hyaluronic acid containing epidermal growth factor to enhance cytokine production by fibroblasts. J Artif Organs. 2013;16(4):489-494.
Goodarzi P, Falahzadeh K, Nematizadeh M, Farazandeh P, Payab M, Larijani B, et al. Tissue Engineered Skin Substitutes. Adv Exp Med Biol. 2018;1107:143-188.
Bainbridge P. Wound healing and the role of fibroblasts. J Wound Care. 2013;22(8):407-412.
Rozman P. Platelet antigens. The role of human platelet alloantigens (HPA) in blood transfusion and transplantation. Transpl Immunol. 2002;10(2-3):165-181.
Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg. 2006;118(6):147e-159e.
Schultz G, Rotatori DS, Clark W. EGF and TGF-alpha in wound healing and repair. J Cell Biochem. 1991;45(4):346-352.
Nolte SV, Xu W, Rennekampff HO, Rodemann HP. Diversity of fibroblasts--a review on implications for skin tissue engineering. Cells Tissues Organs. 2008;187(3):165-176.
Bodnar RJ. Epidermal Growth Factor and Epidermal Growth Factor Receptor: The Yin and Yang in the Treatment of Cutaneous Wounds and Cancer. Adv Wound Care (New Rochelle). 2013;2(1):24-29.
Shamis Y, Silva EA, Hewitt KJ, Brudno Y, Levenberg S, Mooney DJ, et al. Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo. PLoS One. 2013;8(12):e83755.
Kendall RT, Feghali-Bostwick CA. Fibroblasts in fibrosis: novel roles and mediators. Front Pharmacol. 2014;5:123.
Kuroyanagi M, Yamamoto A, Shimizu N, Ishihara E, Ohno H, Takeda A, et al. Development of cultured dermal substitute composed of hyaluronic acid and collagen spongy sheet containing fibroblasts and epidermal growth factor. J Biomater Sci Polym Ed. 2014;25(11):1133-43.
Kuroyanagi M, Kuroyanagi Y. Tissue-engineered products capable of enhancing wound healing. AIMS Mater Sci. 2017;4(3):561–81.

[1] Obagi Z, Damiani G, Grada A, Falanga V. Principles of Wound Dressings: A Review. Surg Technol Int. 2019;35:50-57.

[2] Pugliese E, Coentro JQ, Raghunath M, Zeugolis DI. Wound healing and scar wars. Adv Drug Deliv Rev. 2018;129:1-3.

[3] Hardwicke J, Schmaljohann D, Boyce D, Thomas D. Epidermal growth factor therapy and wound healing--past, present and future perspectives. Surgeon. 2008;6(3):172-177.

[4] Xu P, Wu Y, Zhou L, Yang Z, Zhang X, Hu X, et al. Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization. Burns Trauma. 2020 Aug 14;8:tkaa028.

[5] Yamamoto A, Shimizu N, Kuroyanagi Y. Potential of wound dressing composed of hyaluronic acid containing epidermal growth factor to enhance cytokine production by fibroblasts. J Artif Organs. 2013;16(4):489-494.

[6] Goodarzi P, Falahzadeh K, Nematizadeh M, Farazandeh P, Payab M, Larijani B, et al. Tissue Engineered Skin Substitutes. Adv Exp Med Biol. 2018;1107:143-188.

[7] Bainbridge P. Wound healing and the role of fibroblasts. J Wound Care. 2013;22(8):407-412.

[8] Rozman P. Platelet antigens. The role of human platelet alloantigens (HPA) in blood transfusion and transplantation. Transpl Immunol. 2002;10(2-3):165-181.

[9] Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg. 2006;118(6):147e-159e.

[10] Schultz G, Rotatori DS, Clark W. EGF and TGF-alpha in wound healing and repair. J Cell Biochem. 1991;45(4):346-352.

[11] Nolte SV, Xu W, Rennekampff HO, Rodemann HP. Diversity of fibroblasts--a review on implications for skin tissue engineering. Cells Tissues Organs. 2008;187(3):165-176.

[12] Bodnar RJ. Epidermal Growth Factor and Epidermal Growth Factor Receptor: The Yin and Yang in the Treatment of Cutaneous Wounds and Cancer. Adv Wound Care (New Rochelle). 2013;2(1):24-29.

[13] Shamis Y, Silva EA, Hewitt KJ, Brudno Y, Levenberg S, Mooney DJ, et al. Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo. PLoS One. 2013;8(12):e83755.

[14] Kendall RT, Feghali-Bostwick CA. Fibroblasts in fibrosis: novel roles and mediators. Front Pharmacol. 2014;5:123.

[15] Kuroyanagi M, Yamamoto A, Shimizu N, Ishihara E, Ohno H, Takeda A, et al. Development of cultured dermal substitute composed of hyaluronic acid and collagen spongy sheet containing fibroblasts and epidermal growth factor. J Biomater Sci Polym Ed. 2014;25(11):1133-43.

[16] Kuroyanagi M, Kuroyanagi Y. Tissue-engineered products capable of enhancing wound healing. AIMS Mater Sci. 2017;4(3):561–81.

Epidermal Growth Factor (EGF) representing the role of other growth factors contained in Platelet-Rich Plasma (PRP)

Abstract

References

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