Background: Polyvinyl siloxane has been used by prosthodontists as the impression material for its acceptable dimension stability. Polyvinyl siloxane impression with blood and saliva on it contains bacteria colonization. It has to be disinfected by a disinfectant solution to avoid cross-transmission from patients to the dentist, assistant, and laboratory technician. Nowadays, herbal plant like red fruit (Pandanus conoideus Lam) is utilized as natural disinfection material.
Aim: This study examines the effectiveness of length of disinfection on Pandanus conoideus Lam in polyvinyl siloxane impression.
Method: Thisstudyinvolved four groups (twenty-four teeth impressions) of participants from the Faculty of Dentistry, Universitas Padjadjaran. The first group was disinfected by Pandanus conoideus Lam’s extract 6% for five minutes, ten minutes for the second group, twelve minutes for the third group, and fifteen minutes for the fourth group. Each impression was swabbed before and after being disinfected, planted in Brain Heart Infusion (BHI)agar as the media, incubated for twenty-four hours, and the results of bacteria colonization were counted. Data analysis was evaluated applying paired-t-test in each impression.
Result: There were significant decreases in bacteria colonization numbers between the data before and after the disinfection. The most significant one was found in ten minutes provocation.
Conclusion: Disinfection by Pandanus conoideus Lam’s extract 6% is effective within the range of five to ten minutes and also effective to be used as natural disinfection material for polyvinyl siloxane. The most effective length of disinfection is ten minutes.