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Antioxidant activity in red mulberries on sperm development exposed by cigarette smoke


Introduction: The adverse effects of exposure to cigarette smoke have been discovered for a long time. However, most people assume that exposure to cigarette smoke merely causes respiratory problems. The increase in free radicals from cigarette smoke will also affect sperm development, decreasing the number of sperm produced. The intake of antioxidants derived from red mulberry is expected to reduce cigarette smoke exposure on sperm count. The study aimed to analyze the antioxidant activity of red mulberries on sperm development exposed by cigarette smoke in experimental animals.

Methods: This study employed experimental animals, Wistar Rats (Rattus norvegicus), divided into five groups with different treatments in each group. The treatment in the form of exposure to cigarette smoke and red mulberry was undertaken for 30 days. The parameters used were histological preparations from the testes by calculating the number of Leydig cells, spermatocytes, and spermatids in each tubule. 

Results: The results showed that the negative control group had the highest mean, while the positive control group had the lowest mean. The treatment group showed that the more red mulberry juice was given, the more sperm count was increased. The study group had a significant difference (p <0.05) and a strong relationship between groups (r> 0.80).

Conclusion: In conclusion, consuming red mulberry juice can increase sperm quantity exposed by cigarette smoke.


  1. Hu SS, Neff L, Agaku IT, Cox S, Day HR, Holder-Hayes E, et al. Tobacco product use among adults—United States, 2013–2014. Morb Mortal Wkly Rep. 2016;65(27):685–91.
  2. Benowitz NL, Hukkanen J, Jacob P. Nicotine chemistry, metabolism, kinetics and biomarkers. Nicotine Psychopharmacol. 2009;29–60.
  3. Onor IO, Stirling DL, Williams SR, Bediako D, Borghol A, Harris MB, et al. Clinical effects of cigarette smoking: epidemiologic impact and review of pharmacotherapy options. Int J Environ Res Public Health. 2017;14(10):1147.
  4. Wirjatmadi B, Suryadinata RV. The Alteration on Malondialdehyde Content on Wistar Rats’ Blood and Lungs Tissue to Ward the Exposure of Electric Cigarette Smoke. Indian J Public Heal Res Dev. 2020;11(3):1881–7.
  5. Suryadinata RV, Wirjatmadi B, Adriani M, Sumarmi S. The effects of exposure duration to electronic cigarette smoke on differences in superoxide dismutase and malondialdehyde in blood of wistar rats. Int J Curr Pharm Res. 2019;11(3):13–6.
  6. West R. Tobacco smoking: Health impact, prevalence, correlates and interventions. Psychol Health. 2017;32(8):1018–36.
  7. Dai J-B, Wang Z-X, Qiao Z-D. The hazardous effects of tobacco smoking on male fertility. Asian J Androl. 2015;17(6):954.
  8. Harlev A, Agarwal A, Gunes SO, Shetty A, du Plessis SS. Smoking and male infertility: an evidence-based review. World J Mens Health. 2015;33(3):143.
  9. Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 2018;16(1):35–43.
  10. Practice Committee of the American Society for Reproductive Medicine. Smoking and infertility: a committee opinion. Fertil Steril. 2018;110(4):611–8.
  11. Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. J Funct Foods. 2015;18:820–97.
  12. Comunian TA, Ravanfar R, de Castro IA, Dando R, Favaro-Trindade CS, Abbaspourrad A. Improving oxidative stability of echium oil emulsions fabricated by Microfluidics: Effect of ionic gelation and phenolic compounds. Food Chem. 2017;233:125–34.
  13. Indraswari PI, Lorensia A, Suryadinata RV. Analysis Effect of Nutrition Intake on Lung Function of Active Smoker and Non Smoker. J Kesehat Masy. 2018;14(2):247–53.
  14. Tvrdá E, K?ažická Z, Bárdos L, Massányi P, Luká? N. Impact of oxidative stress on male fertility—A review. Acta Vet Hung. 2011;59(4):465–84.
  15. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. 2017;2017.
  16. Tan BL, Norhaizan ME, Liew W-P-P, Sulaiman Rahman H. Antioxidant and oxidative stress: a mutual interplay in age-related diseases. Front Pharmacol. 2018;9:1162.
  17. Pratiwi SR, Lorensia A, Suryadinata RV. Asupan Vitamin C dan E dengan SQ-FFQ terhadap Fungsi Paru Perokok dan Non-Perokok= Vitamin C and E Intake with SQ-FFQ towards Smokers’ and Non-Smokers’ Lung Function. Media Kesehat Masy Indones. 2018;14(2):101–7.
  18. Kumar S, Murarka S, Mishra V V, Gautam AK. Environmental & lifestyle factors in deterioration of male reproductive health. Indian J Med Res. 2014;140(Suppl 1):S29.
  19. Gandhi J, Hernandez RJ, Chen A, Smith NL, Sheynkin YR, Joshi G, et al. Impaired hypothalamic-pituitary-testicular axis activity, spermatogenesis, and sperm function promote infertility in males with lead poisoning. Zygote. 2017;25(2):103–10.
  20. Cheng CY, Mruk DD. The biology of spermatogenesis: the past, present and future. The Royal Society; 2010.
  21. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal. 2013;162750.
  22. Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. Journal of nutritional science. 2016;5:e47.
  23. Jamalan M, Ghaffari MA, Hoseinzadeh P, Hashemitabar M, Zeinali M. Human Sperm Quality and Metal Toxicants: Protective Effects of some Flavonoids on Male Reproductive Function. International journal of fertility & sterility. 2016;10(2):215–223.
  24. Huyut Z, Beydemir ?, Gülçin ?. Antioxidant and antiradical properties of selected flavonoids and phenolic compounds. Biochem Res Int. 2017;2017.
  25. Suryadinata RV, Wirjatmadi B. The Molecular Pathways of Lung Damage by E-Cigarette in Experimental Mice. Sultan Qaboos University Medical Journal . 2021

How to Cite

Suryadinata, R. V., Sukarno, D. A., Sardjono, S. C., & Adriani, M. (2021). Antioxidant activity in red mulberries on sperm development exposed by cigarette smoke. Bali Medical Journal, 10(2), 583–586.




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Rivan Virlando Suryadinata
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Devitya Angielevi Sukarno
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Stefani Cornelia Sardjono
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Merryana Adriani
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