Skip to main content Skip to main navigation menu Skip to site footer

Melatonin prevented the increased serum lactate levels and the reduced hemoglobin levels in burn-induced Wistar rats

  • Graciela Dhea ,
  • Sulistiyati Bayu Utami ,
  • Faizah Fulyani ,
  • Erwin Kresnoadi ,
  • Satrio Adi Wicaksono ,


Abstract

Background: Burns are the most common and devastating form of trauma yet constitute a common cause of morbidity worldwide. Complications of burns such as sepsis and tissue hypoperfusion may increase serum lactate and reduce hemoglobin. Measuring lactate levels has been proven to be a useful predictive marker of hypoperfusion and sepsis in patients with burns. Melatonin which acts as an anti-inflammatory and antioxidant, has been proposed to be used as pharmacological adjuvant in patients with burns, reducing oxidative damage. The study aims to investigate the effects of melatonin supplementation on serum lactate and hemoglobin levels in burn-induced male Wistar rats.

Method: This was an experimental animal study with randomized control group design. All samples (n=12 male Wistar rats) were randomized and divided into two groups. Each rat was exposed to a hot metal rod for 10 seconds to induce 30% burns injury. Rats in the control group were administered with placebo (aquadest), while rats in the experimental group were administered with melatonin intraperitoneally at 0, 8, and 16 hours after burns injury. Blood samples were collected from the retro-orbital sinuses at 0, 3, and 24 hours after treatment. Data were statistically analyzed using Paired t-Test, Independent t-Test Mann-Whitney Rank Test and Wilcoxon test.

Results: There were no significant differences in lactate and hemoglobin levels between melatonin group and control group 0 hours after treatment (T0), 3 hours after treatment (T3), and 24 hours after treatment (T24) (p>0.05). There were increased lactate levels and decreased hemoglobin levels in both melatonin and control groups, however the increment of lactate and the decrement of hemoglobin were steeper in control group compared to melatonin group. There were higher serum lactate increments and higher hemoglobin decrement at 24 hours (∆T0–T24) in control compared to melatonin group (p<0.05).

Conclusion: Melatonin might prevent dramatic rise of serum lactate levels and decrease of hemoglobin levels until 24 hours after treatment in burns injury. If confirmed by further studies, melatonin might have a role as adjuvant therapy in burns.

Keywords: burns injury serum lactate level hemoglobin melatonin.

References

  1. Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2006;19(2):403-34.
  2. Lim JJ, Rehmar SG, Elmore P. Rapid response: Care of burn victims. AAOHN Journal. 1998;46(4):169-78.
  3. Ministry of health Republic of Indonesia. Indonesian basic health research. 2018. Jakarta: Health research and development agency. Ministry of Health, Republic of Indonesia; 2018.
  4. Garcia-Alvarez M, Marik P, Bellomo R. Sepsis-associated hyperlactatemia. Crit Care 2014;18(5):503.
  5. Andersen LW, Mackenhauer J, Roberts JC, Berg KM, Cocchi MN, Donnino MW. Etiology and therapeutic approach to elevated lactate. Mayo Clin Proc. 2013;88(10):1127-40.
  6. Mokline A, Abdenneji A, Rahmani I, Gharsallah L, Tlaili S, Harzallah I, et al. Lactate: Prognostic biomarker in severely burned patients. Ann Burns Fire Disasters. 2017;30(1):35-8.
  7. Kamolz LP, Andel H, Schramm W, Meissl G, Herndon DN, Frey M. Lactate: Early predictor of morbidity and mortality in patients with severe burns. Burns. 2005;31(8):986-90.
  8. Cochran A, Edelman LS, Saffle JR, Morris SE. The relationship of serum lactate and base deficit in burn patients to mortality. J Burn Care Res. 2007;28(2):231-40.
  9. Faix JD. Biomarkers of sepsis. Crit Rev Clin Lab Sci. 2013;50(1):23-36.
  10. Broder G, Weil MH. Excess lactate: An index of reversibility of shock in human patients. Science. 1964;143(3613):1457-9.
  11. Posluszny JA, Gamelli RL. Anemia of thermal injury: Combined acute blood loss anemia and anemia of critical illness. J Burn Care Res. 2010;31(2):229-42.
  12. Kurdi MS, Patel T. The role of melatonin in anesthesia and critical care. Indian J Anaesth. 2013;57(2):137-44.
  13. Şener G, Şehirli AÖ, Şatıroğlu H, Keyer-Uysal M, Yeğen BÇ. Melatonin improves oxidative organ damage in a rat model of thermal injury. Burns. 2002;28(5):419-25.
  14. Maldonado M-D, Murillo-Cabezas F, Calvo J-R, Lardone P-J, Tan D-X, Guerrero J-M, et al. Melatonin as pharmacologic support in burn patients: A proposed solution to thermal injury-related lymphocytopenia and oxidative damage. Crit Care Med. 2007;35(4):1177-85.
  15. Strudwick XL, Cowin AJ. The role of the inflammatory response in burn injury. Hot topics in burn injuries. Adelaide, South Australia, Australia: Intechopen; 2018:37-57.
  16. Reiter RJ, Tan D-X, Fuentes-Broto L. Melatonin: A multitasking molecule. Progress Brain Res. 2010;181:127-51.
  17. Wicaksono SA, Kumalasari D, Valeria A, Utami SB. Melatonin and polymyxin b administration, alone or in combination, prevent the elevation of random blood glucose and lactate levels in wistar rats endotoxicosis model. Pakistan J Med Health Sci. 2020;14(2):1082-90.
  18. Wicaksono SA, Harisiani DA, Riani RI, Utami SB. An adjuvant treatment of melatonin prevented the elevation of leukocyte count and the decrease of platelet count in wistar rats endotoxicosis model. Pakistan J Med Health Sci. 2020;14(2):705-12.
  19. Kaya O, Gokdemir K, Kilic M, Baltaci A. Melatonin supplementation to rats subjected to acute swimming exercise: Its effect on plasma lactate levels and relation with zinc. Neuro Endocrinol Lett. 2006;27(1-2):263-66.
  20. Dalton KM. Effects of melatonin on hemolymph glucose and lactate concentrations in the fiddler crab, uca pugilator. Department of Chemistry. 2002;In Partial Fulfillment of the Requirements for Graduation With Honors in Chemistry

How to Cite

Dhea, G., Utami, S. B., Fulyani, F., Kresnoadi, E., & Wicaksono, S. A. (2021). Melatonin prevented the increased serum lactate levels and the reduced hemoglobin levels in burn-induced Wistar rats. Bali Medical Journal, 10(1), 331–335. https://doi.org/10.15562/bmj.v10i1.2179
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
0

Total
13

Share

Search Panel

Graciela Dhea
Google Scholar
Pubmed
BMJ Journal

Sulistiyati Bayu Utami
Google Scholar
Pubmed
BMJ Journal

Faizah Fulyani
Google Scholar
Pubmed
BMJ Journal

Erwin Kresnoadi
Google Scholar
Pubmed
BMJ Journal

Satrio Adi Wicaksono
Google Scholar
Pubmed
BMJ Journal