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The effect of duration of wound skin tissue on MDA, TNF-α, IL-6, Caspase 3, VEGF levels, and granulation tissue thickness in the white rat (Rattus novergicus)

  • Amru Sungkar ,
  • Danar Widyatmoko ,
  • Kristanto Yuli Yarso ,
  • Brian Wasita ,


Introduction: Wound is the result of physical trauma that severs skin continuities. The damage typically causes an inflammatory reaction marked by increase ROS and tissue inflammation. However, this process is still needed to be further investigated. Therefore, this study aimed to evaluate plasma MDA, IL-6, TNF-α, Caspase-3 and VEGF levels, and the granulation tissue thickness at different time points during the wound healing process.

Methods: An experimental post-test only control group study was conducted using white male rats (Rattus Norvegicus) and the rats were wounded and assessed on day 5 (K1), day 10 (K2), and day 15 (K3). The level of plasma MDA, IL-6, TNF-α, Caspase-3 and VEGF level was assessed as well as the granulation tissue thickness.

Results: The OneWay ANOVA test results showed significant differences in plasma MDA, IL-6, TNF-α, Caspase-3 and VEGF levels (P <0.05). The Tukey HSD test results showed significant differences between (P <0.05) K1 with K2, K1 and K3 and K2 with K3 on MDA plasma, IL-6, TNF-α, Caspase-3 and VEGF. However, the granulation tissue thickness was only slightly different between groups and was not significant.

Conclusion: The inflammation and angiogenesis were steadily increased overtime during the wound healing process while oxidative stress and cell death were decreasing. However, none of those factors were related to granulation thickness.


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How to Cite

Sungkar, A., Widyatmoko, D., Yarso, K. Y., & Wasita, B. (2020). The effect of duration of wound skin tissue on MDA, TNF-α, IL-6, Caspase 3, VEGF levels, and granulation tissue thickness in the white rat (Rattus novergicus). Bali Medical Journal, 9(3), 918–923.




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