The effect of ethyl acetate fraction of Moringa oleifera leaves on neutrophil and MDA levels in the improvement of liver dysfunction in male rats with sepsis model

Riswanto Riswanto; Tatar Sumandjar; Dhani Redhono; Robby Kurniawan; Arief Rahman

doi:10.15562/bmj.v9i3.1850

The effect of ethyl acetate fraction of Moringa oleifera leaves on neutrophil and MDA levels in the improvement of liver dysfunction in male rats with sepsis model

Riswanto Riswanto ,

Tatar Sumandjar ,

Dhani Redhono ,

Robby Kurniawan ,

Arief Rahman ,

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DOI: https://doi.org/10.15562/bmj.v9i3.1850 |
Published: 2020-12-01

Abstract

Purpose: Sepsis is life-threatening organ dysfunction caused by the dysregulation of the host response due to infection. Sepsis is caused by an imbalance between pro-inflammatory and anti-inflammatory cytokines. Moringa oleifera (Mo) leaves naturally contains antiinflammation and antioxidant. We aim to investigate Mo leaves in sepsis.

Patients and methods: This is an experimental laboratory study with Post Test Only Control Group Design. 30 Winstar males rats were divided into four treatment groups; each consist of 6 rats. The negative group, the positive control group received an injection of LPS dose of 0.25 mg/kg BW, and the interventional group with Mo-EA dose of 10, 20, 40 mg/kg BW. The Annova and Kruskal-Wallis test was used in this study. The significant p-value<0.05. The measured outcome were neutrophil levels, malondialdehyde (MDA) levels, and Serum Glutamic Pyruvate Transaminase (SGPT) / Serum Glutamic Oxaloacetic Transaminase (SGOT).

Results: The neutrophil, MDA, SGOT, and SGPT levels were decreased significantly within the treated group. MO Fraction can decrease the serum of MDA, Neutrophil, and SGOT/SGPT levels in days 3 and 7 (p<0.05).

Conclusion: There was a decrease in the level of Neutrophil, MDA, SGOT, and SGPT improvement of Hepar organ dysfunction after administration of MO Extract, so it is useful to inhibit the progression of sepsis.

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