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The hepatoprotective effect of cacao beans whole extracts (Theobroma cacao L.) in oxidative stress mice



Abstract

Background: Increased production of reactive oxygen species (ROS), causing accumulation of oxidative damage. Carbontetraclorida (CCl4) is known as a toxic material and causing hepatocellular damage initiated by oxidative stress, which increased blood serum glutamic pyruvic transaminase (SGPT) concentration. Cacao beans (Theobroma cacao L.) extracts have antioxidant properties from flavonols, consisting of catechin, epicatechin, and procyanidin. This study aims to investigate the effect of hepatoprotective of cacao beans (Theobroma cacao L.) extracts in oxidative stress mice induced by CCL4. 

Methods: The study was conducted at the Department of Biochemistry and Department of Pharmacology and Therapy, Faculty of Medicine Udayana University using the Pretest-Postest Control Group Design. About 20 male mice were divided into 4 groups, as control or placebo group (P0), intervention group by 15 mg extracts of cacao beans (P1), 30 mg (P2), and 60 mg (P3) for 7 days. On the eight-day induced by 0,55 mg/gram mice bodyweight of CCl4. All groups were examined for blood SGPT concentration before and after the intervention. Data were analyzed using SPSS version 20 for Windows.

Results: The SGPT level prior to the administration of cacao bean extract and CCl4 was 18.56±0.19 µ/L in P0 group, followed by 18.41±0.28 µ/L (P1), 18.36±0.21 µ/L (P2), and 18.16±0.47 µ/L (P3), but statistically not significant (p=0.260). However, after CCl4 administration, the SGPT levels were 38.63±0.37 µ/L in P0 but significant decrease in the P1 (28.14±0.34 µ/L), P2 (20.72±0.35 µ/L), and P3 (18.68±0.25 µ/L) following cacao beans whole extracts administration (p=0.000).

Conclusion: This study concluded that cacao beans extracts administration could inhibit the oxidative stress and protect hepatocellular damage induced by CCl4 by decreasing the blood SGPT concentration.

Keywords: CCl4 Oxidative Stress Cacao Beans Flavanols SGPT

References

  1. Corless JK, Middleton HM 3rd. Normal liver function. A basis for understanding hepatic disease. Arch Intern Med. 1983;143(12):2291-2294.
  2. Spitz DR, Azzam EI, Li JJ, Gius D. Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: a unifying concept in stress response biology. Cancer Metastasis Rev. 2004;23(3-4):311-322.
  3. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-126.
  4. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcocaci V, et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017:8416763.
  5. Tan BL, Norhaizan ME, Liew WP, Sulaiman Rahman H. Antioxidant and Oxidative Stress: A Mutual Interplay in Age-Related Diseases. Front Pharmacol. 2018;9:1162.
  6. Alkadi H. A Review on Free Radicals and Antioxidants. Infect Disord Drug Targets. 2020;20(1):16-26.
  7. Harman D. The aging process: major risk factor for disease and death. Proc Natl Acad Sci U S A. 1991;88(12):5360-5363.
  8. Han RM, Zhang JP, Skibsted LH. Reaction dynamics of flavonoids and carotenoids as antioxidants. Molecules. 2012;17(2):2140-2160.
  9. Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A. Biomarkers of oxidative damage in human disease. Clin Chem. 2006;52(4):601-623.
  10. Baba S, Osakabe N, Kato Y, Natsume M, Yasuda A, Kido T, et al. Continuous intake of polyphenolic compounds containing cocoa powder reduces LDL oxidative susceptibility and has beneficial effects on plasma HDL-cholesterol concentrations in humans. Am J Clin Nutr. 2007;85(3):709-717.
  11. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res. 1995;22(4):375-383. doi:10.3109/10715769509145649
  12. Jia R, Cao LP, Du JL, Wang JH, Liu YJ, Jeney G, et al. Effects of carbon tetrachloride on oxidative stress, inflammatory response and hepatocyte apoptosis in common carp (Cyprinus carpio). Aquat Toxicol. 2014;152:11-19.
  13. MacDonald-Wicks LK, Garg ML. Modulation of carbon tetrachloride-induced oxidative stress by dietary fat in rats(open star). J Nutr Biochem. 2002;13(2):87-95.
  14. Weinberger B, Watorek K, Strauss R, Witz G, Hiatt M, Hegyi T. Association of lipid peroxidation with hepatocellular injury in preterm infants. Crit Care. 2002;6(6):521-525.
  15. Gowda S, Desai PB, Hull VV, Math AA, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. Pan Afr Med J. 2009;3:17. Published 2009 Nov 22.
  16. Arlorio M, Bottini C, Travaglia F, Locatelli M, Bordiga M, Coisson JD, et al. Protective activity of Theobroma cacao L. phenolic extract on AML12 and MLP29 liver cells by preventing apoptosis and inducing autophagy. J Agric Food Chem. 2009;57(22):10612-10618.
  17. Sibulesky L. Normal liver anatomy. Clin Liver Dis (Hoboken). 2013;2(Suppl 1):S1-S3.
  18. Crawford AR, Lin XZ, Crawford JM. The normal adult human liver biopsy: a quantitative reference standard. Hepatology. 1998;28(2):323-331.
  19. Sell S. The hepatocyte: heterogeneity and plasticity of liver cells. Int J Biochem Cell Biol. 2003;35(3):267-271.
  20. Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, et al. The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int J Mol Sci. 2015;16(11):26087-26124.
  21. Arcana N, Adioka GM, Wihandani DM. Daya Hepatoprotektor Ekstrak Buah Jambu Biji, Buni, dan Wortel Pada Mencit yang Diinduksi dengan Carbontetrachlorida. Medicina. 2008;39(1):1-8.
  22. Cichoż-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol. 2014;20(25):8082-8091.
  23. Katz DL, Doughty K, Ali A. Cocoa and chocolate in human health and disease. Antioxid Redox Signal. 2011;15(10):2779-2811. doi:10.1089/ars.2010.3697
  24. Nabavi SF, Sureda A, Daglia M, Rezaei P, Nabavi SM. Anti-Oxidative Polyphenolic Compounds of Cocoa. Curr Pharm Biotechnol. 2015;16(10):891-901.
  25. Muliarta M, Tirtayasa K, Prabawa PY, Wiryadana KA. Tamarillo Consumption Associated with Increased Acetylcholinesterase Activity and Improved Oxidative Stress Markers in Farmers Exposed to Daily Pesticide-related Activities in Baturiti, Bali, Indonesia. Open Access Maced J Med Sci. 2020;8(E):244-250.

How to Cite

Wiryanthini, I. A. D., Sutadarma, I. W. G., Dewi, N. W. S., & Surudarma, I. W. (2020). The hepatoprotective effect of cacao beans whole extracts (Theobroma cacao L.) in oxidative stress mice. Bali Medical Journal, 9(3), 656–659. https://doi.org/10.15562/bmj.v9i3.1789
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Ida Ayu Dewi Wiryanthini
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I Wayan Gede Sutadarma
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Ni Wayan Sucindra Dewi
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I Wayan Surudarma
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