The effect of Balinese Purple Sweet Potato (Ipomoea batatas L.) tuber ethanolic extract on renal Nox4 level in mice with a high purine diet

Agung Nova Mahendra; Ni Wayan Sucindra Dewi

doi:10.15562/bmj.v9i3.1787

The effect of Balinese Purple Sweet Potato (Ipomoea batatas L.) tuber ethanolic extract on renal Nox4 level in mice with a high purine diet

Agung Nova Mahendra ,

Ni Wayan Sucindra Dewi ,

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DOI: https://doi.org/10.15562/bmj.v9i3.1787 |
Published: 2020-10-13

Abstract

Background: TLR4-NADPH Oxidase-4 or TLR4-Nox4 signaling pathway uric acid (UA)-induced oxidative stress is involved in urate nephropathy pathogenesis, which can culminate into renal fibrosis, a common feature of chronic kidney disease (CKD). Based on the hyperuricemic and antioxidant effect of anthocyanin on the kidney, we assumed that Balinese purple sweet potato (Ipomoea batatas L.) tuber in its ethanolic extract form (PSPEE) could suppress the activation of renal TLR4-Nox4 signaling. This study was aimed to investigate the renoprotective effect of PSPEE via renal Nox4 downregulation, using a murine model induced with a high-purine diet.

Methods: This study was an experimental laboratory study using a post-test only with a control group design among 38 male Swiss mice. The Male Swiss mice (20-30 g) were randomly allocated into 2 groups, namely, the control group (treated with a high-purine diet for 7 days) and the PSPEE group (treated with a high-purine diet for 7 days and PSPEE for 14 days). The serum uric acid (sUA) level was quantified using the UA phosphatase method. Meanwhile, the renal Nox4 level was quantified using ELISA. Data were analyzed using SPSS version 20 for Windows.

Results: Our study found that the renal Nox4 levels were 18.65Â±1.67 ng/mL and 15.14Â±1.66 ng/mL for the control and PSPEE groups. Based on the statistical analysis, there was no statistically significant difference between control and PSPEE groups (p>0.05).

Conclusion: Balinese PSPEE tends to decrease renal Nox4 level in high-purine diet-treated mice, but fails to show a significant effect.

References

Johnson RJ, Nakagawa T, Jalal D, SÃ¡nchez-Lozada LG, Kang DH, Ritz E. Uric acid and chronic kidney disease: which is chasing which?. Nephrol Dial Transplant. 2013;28(9):2221-2228.
Kim IY, Lee DW, Lee SB, Kwak IS. The role of uric acid in kidney fibrosis: experimental evidences for the causal relationship. Biomed Res Int. 2014;2014:638732.
Gill R, Tsung A, Billiar T. Linking oxidative stress to inflammation: Toll-like receptors. Free Radic Biol Med. 2010;48(9):1121-1132.
Li Z, Sheng Y, Liu C, Li K, Huang X, Huang J, et al. Nox4 has a crucial role in uric acid induced oxidative stress and apoptosis in renal tubular cells. Mol Med Rep. 2016;13(5):4343-4348.
Milanesi S, Verzola D, Cappadona F, Bonino B, Murugavel A, Pontremoli R, et al. Uric acid and angiotensin II additively promote inflammation and oxidative stress in human proximal tubule cells by activation of toll-like receptor 4. J Cell Physiol. 2019;234(7):10868-10876.
Satriyasa BK. Aqueous extract of purple sweet potato tubers decreases MDA and increases SOD2 in kidney of diabetic rats. Bali Med J. 2016;5(3):388-390.
Gorin Y. Nox4 as a potential therapeutic target for treatment of uremic toxicity associated to chronic kidney disease. Kidney Int. 2013;83(4):541-543.
Jeong BY, Lee HY, Park CG, Kang J, Yu SL, Choi DR, et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One. 2018;13(1):e0191034.
Canugovi C, Stevenson MD, Vendrov AE, Hayami T, Robidoux J, Xiao H, et al. Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox Biol. 2019;26:101288.
Zhang Y, Neogi T, Chen C, Chaisson C, Hunter DJ, Choi HK. Cherry consumption and decreased risk of recurrent gout attacks. Arthritis Rheum. 2012;64(12):4004-4011.
Zhang ZC, Su GH, Luo CL, Pang YL, Wang L, Li X, et al. Effects of anthocyanins from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8) on the serum uric acid level and xanthine oxidase activity in hyperuricemic mice. Food Funct. 2015;6(9):3045-3055.
Qian X, Wang X, Luo J, Liu Y, Pang J, Zhang H, et al. Hypouricemic and nephroprotective roles of anthocyanins in hyperuricemic mice. Food Funct. 2019;10(2):867-878.
Oshima N, Onimaru H, Matsubara H, Uchida T, Watanabe A, Takechi H, et al. Uric acid, indoxyl sulfate, and methylguanidine activate bulbospinal neurons in the RVLM via their specific transporters and by producing oxidative stress. Neuroscience. 2015;304:133-145.

[1] Johnson RJ, Nakagawa T, Jalal D, SÃ¡nchez-Lozada LG, Kang DH, Ritz E. Uric acid and chronic kidney disease: which is chasing which?. Nephrol Dial Transplant. 2013;28(9):2221-2228.

[2] Kim IY, Lee DW, Lee SB, Kwak IS. The role of uric acid in kidney fibrosis: experimental evidences for the causal relationship. Biomed Res Int. 2014;2014:638732.

[3] Gill R, Tsung A, Billiar T. Linking oxidative stress to inflammation: Toll-like receptors. Free Radic Biol Med. 2010;48(9):1121-1132.

[4] Li Z, Sheng Y, Liu C, Li K, Huang X, Huang J, et al. Nox4 has a crucial role in uric acid induced oxidative stress and apoptosis in renal tubular cells. Mol Med Rep. 2016;13(5):4343-4348.

[5] Milanesi S, Verzola D, Cappadona F, Bonino B, Murugavel A, Pontremoli R, et al. Uric acid and angiotensin II additively promote inflammation and oxidative stress in human proximal tubule cells by activation of toll-like receptor 4. J Cell Physiol. 2019;234(7):10868-10876.

[6] Satriyasa BK. Aqueous extract of purple sweet potato tubers decreases MDA and increases SOD2 in kidney of diabetic rats. Bali Med J. 2016;5(3):388-390.

[7] Gorin Y. Nox4 as a potential therapeutic target for treatment of uremic toxicity associated to chronic kidney disease. Kidney Int. 2013;83(4):541-543.

[8] Jeong BY, Lee HY, Park CG, Kang J, Yu SL, Choi DR, et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One. 2018;13(1):e0191034.

[9] Canugovi C, Stevenson MD, Vendrov AE, Hayami T, Robidoux J, Xiao H, et al. Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox Biol. 2019;26:101288.

[10] Zhang Y, Neogi T, Chen C, Chaisson C, Hunter DJ, Choi HK. Cherry consumption and decreased risk of recurrent gout attacks. Arthritis Rheum. 2012;64(12):4004-4011.

[11] Zhang ZC, Su GH, Luo CL, Pang YL, Wang L, Li X, et al. Effects of anthocyanins from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8) on the serum uric acid level and xanthine oxidase activity in hyperuricemic mice. Food Funct. 2015;6(9):3045-3055.

[12] Qian X, Wang X, Luo J, Liu Y, Pang J, Zhang H, et al. Hypouricemic and nephroprotective roles of anthocyanins in hyperuricemic mice. Food Funct. 2019;10(2):867-878.

[13] Oshima N, Onimaru H, Matsubara H, Uchida T, Watanabe A, Takechi H, et al. Uric acid, indoxyl sulfate, and methylguanidine activate bulbospinal neurons in the RVLM via their specific transporters and by producing oxidative stress. Neuroscience. 2015;304:133-145.

The effect of Balinese Purple Sweet Potato (Ipomoea batatas L.) tuber ethanolic extract on renal Nox4 level in mice with a high purine diet

Abstract

References

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