The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model

Tatar Sumandjar; Bambang Purwanto; Brian Wasita; Dono Indarto; Risya Cilmiaty; Vitri Widyaningsih

doi:10.15562/bmj.v8i3.1679

The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model

Tatar Sumandjar ,

Bambang Purwanto ,

Brian Wasita ,

Dono Indarto ,

Risya Cilmiaty ,

Vitri Widyaningsih ,

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DOI: https://doi.org/10.15562/bmj.v8i3.1679 |
Published: 2019-12-26

Abstract

Background: Sepsis is a life-threatening organ dysfunction caused by the dysregulation of the host response to infection, facilitated by inflammation and endothelial stress. Moringa oleifera leaves fractioned with ethyl acetate (MO-EA fraction) is proposed to have the ability to suppress inflammation and oxidative stress through the control of NF-kB. This study aims to investigate the effect of the MO-EA fraction in a rat sepsis model.

Material and Methods: The study is a laboratory experimental study. The research was conducted on a total of 30 rats, equally divided into five groups. One group did not receive lipopolysaccharide (LPS) induction as negative control group, but the others received LPS induction and received variable doses of MO-EA fraction (0, 10, 20, and 40 mg/kg of bodyweight). The measured outcome were serum concentration of of heparanase, CRP, malondialdehyde (MDA), and immunohistologic expression of e-selectin, NF-kB in cells, and histopathological necrosis in the aorta and kidney.

Results: MO-EA fraction significantly decrease the serum levels of HPA, MDA on day 3 and 7, and lower the CRP serum level on day 7 (p<0.05). Other variables of interest did not show significant differences.

Conclusion: The administration of MO-EA fraction of any dose significantly lower the serum level of HPA and MDA in mice sepsis models on day 3 and 7, and lower CRP on day 3 but not on day 7. However, examnationa of NF-kB and e-selectin expressions, and necrosis in kidney proximal tubule and aortic endothelial cells did not show the benefit of MO-EA fraction in preventing cell damage.

References

Sat Sharma MD, Anand Kumar MD. Antimicrobial Management of Sepsis and Septic Shock. 2008. Volume 29, Issue 4, December 2008, Pages 677-687. doi.org/10.1016/j.ccm.2008.06.004
Rami Namas, M.D., Ruben Zamora, Ph.D., Rajaie Namas, M.D.,Gary An, M.D., John Doyle, Ph.D., Thomas E. Dick, Ph.D.,Frank J. Jacono, M.D., Ioannis P. Androulakis, Ph.D., Gary F. Nieman, Ph.D., Steve Chang, M.S., Timothy R. Billiar, M.D., John A. Kellum, M.D., Derek C. Angus, M.D., M.P.H, FRCP, and Yoram Vodovotz, Ph.D. 2012. Sepsis: something old, something new, and a system view, J Crit Care 27:314.e1-314.e11.
Lawrence T, 2009. IKKÎ± in the regulation of inflammation and adaptive immunity. Biochem Soc Trans 35:270â€“272.
Chelazzi C., Villa G., Marcinelli P., De Gaudio A. R., Adembri C. Glicocalyx and sepsis-induced alterations in vaskular permeability. Critical Care. 2015;19(1, article 26) doi: 10.1186/s13054-015-0741-z.
Guntur HA. Immunology, Diagnosis and Management of Sepsis. Low Dose Steroids in the Management of Sepsis. Surakarta: UNS Press; 2011. p. 1-45.
Eroschenko VP. DiFiore's atlas of histology with functional correlations: Lippincott Williams & Wilkins; 2008.
Cui N, Wang H, Long Y, Su L, Liu D. Dexamethasone suppressed LPS-induced matrix metalloproteinase and its effect on endothelial glycocalyx shedding. Mediators of inflammation. 2015;2015.
Arulselvan P, Tan W, Gothai S, Muniandy K, Fakurazi S, Esa N, et al. Anti-inflammatory potential of ethyl acetate fraction of Moringa oleifera in downregulating the NF-kB signaling pathway in lipopolysaccharide-stimulated macrophages. Molecules. 2016;21(11):1452.
Doi K, Leelahavanichkul A, Yuen PS, Star RA. Animal models of sepsis and sepsis-induced kidney injury. The Journal of clinical investigation. 2009;119(10):2868-78.
Cummings CJ, Sessler CN, Beall LD, Fisher BJ, Best AM, FOWLER III AA. Soluble E-selectin levels in sepsis and critical illness: correlation with infection and hemodynamic dysfunction. American journal of respiratory and critical care medicine. 1997;156(2):431-7.
Abdollahi A, Shoar S, Nayyeri F, Shariat M. Diagnostic value of simultaneous measurement of procalcitonin, interleukin-6 and hs-CRP in prediction of early-onset neonatal sepsis. Mediterranean journal of hematology and infectious diseases. 2012;4(1).

[1] Sat Sharma MD, Anand Kumar MD. Antimicrobial Management of Sepsis and Septic Shock. 2008. Volume 29, Issue 4, December 2008, Pages 677-687. doi.org/10.1016/j.ccm.2008.06.004

[2] Rami Namas, M.D., Ruben Zamora, Ph.D., Rajaie Namas, M.D.,Gary An, M.D., John Doyle, Ph.D., Thomas E. Dick, Ph.D.,Frank J. Jacono, M.D., Ioannis P. Androulakis, Ph.D., Gary F. Nieman, Ph.D., Steve Chang, M.S., Timothy R. Billiar, M.D., John A. Kellum, M.D., Derek C. Angus, M.D., M.P.H, FRCP, and Yoram Vodovotz, Ph.D. 2012. Sepsis: something old, something new, and a system view, J Crit Care 27:314.e1-314.e11.

[3] Lawrence T, 2009. IKKÎ± in the regulation of inflammation and adaptive immunity. Biochem Soc Trans 35:270â€“272.

[4] Chelazzi C., Villa G., Marcinelli P., De Gaudio A. R., Adembri C. Glicocalyx and sepsis-induced alterations in vaskular permeability. Critical Care. 2015;19(1, article 26) doi: 10.1186/s13054-015-0741-z.

[5] Guntur HA. Immunology, Diagnosis and Management of Sepsis. Low Dose Steroids in the Management of Sepsis. Surakarta: UNS Press; 2011. p. 1-45.

[6] Eroschenko VP. DiFiore's atlas of histology with functional correlations: Lippincott Williams & Wilkins; 2008.

[7] Cui N, Wang H, Long Y, Su L, Liu D. Dexamethasone suppressed LPS-induced matrix metalloproteinase and its effect on endothelial glycocalyx shedding. Mediators of inflammation. 2015;2015.

[8] Arulselvan P, Tan W, Gothai S, Muniandy K, Fakurazi S, Esa N, et al. Anti-inflammatory potential of ethyl acetate fraction of Moringa oleifera in downregulating the NF-kB signaling pathway in lipopolysaccharide-stimulated macrophages. Molecules. 2016;21(11):1452.

[9] Doi K, Leelahavanichkul A, Yuen PS, Star RA. Animal models of sepsis and sepsis-induced kidney injury. The Journal of clinical investigation. 2009;119(10):2868-78.

[10] Cummings CJ, Sessler CN, Beall LD, Fisher BJ, Best AM, FOWLER III AA. Soluble E-selectin levels in sepsis and critical illness: correlation with infection and hemodynamic dysfunction. American journal of respiratory and critical care medicine. 1997;156(2):431-7.

[11] Abdollahi A, Shoar S, Nayyeri F, Shariat M. Diagnostic value of simultaneous measurement of procalcitonin, interleukin-6 and hs-CRP in prediction of early-onset neonatal sepsis. Mediterranean journal of hematology and infectious diseases. 2012;4(1).

The ethyl acetate fraction of Moringa oleifera leaves effects on endothelial stress in rat sepsis model

Abstract

References

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