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Characterizing the SLC39A14 zinc transporter and collagen products of the chondrocytes from the human umbilical cord-derived mesenchymal stromal cell

  • Ketut Dewi Kumara Wati ,
  • Endah Dianty Pratiwix ,
  • Yanni Dirgantara ,
  • Cynthia Retna Sartika ,
  • Meita Dhamayanti ,
  • Budi Setiabudiawa ,
  • Ida Parwati ,


Abstract

Background: The SLC39A14 zinc transporter showed a role in the growth of the mouse model. There was no report regarding SLC39A14 protein expression in human chondrocyte, which might prevent further identification of SLC39A14’s function in human growth. This study aims to describe the SLC39A14 protein expression in chondrocyte using human umbilical cord (UC)-derived mesenchymal stromal cell (MSC) isolation and differentiation.

Methods: A cross sectional study was conducted at the Faculty of Medicine, Universitas Padjajaran Bandung, Indonesia, in 2019. The UC was derived after the respondents signed informed consents were documented. Specimens transferred, UC tissue digestion, cell isolation, cultures, and passages were done under the laboratory standard protocols. The immuno-phenotyping and differentiation toward chondrocyte procedure were done after third passage. The SLC39A14 expression of the chondrocytes was observed under immunofluorescence microscopy after staining using polyclonal anti SLC39A14 antibody. The collagen 2A and 10A production was measured using the medium supernatant and analyzed using GraphPad Prism 8 edition.

Results: The adherent fibroblast-like cells appeared on day 5th, which continued to proliferate and showed MSC characteristic with positive markers for CD 73, CD90 and CD105, but absent for negative lineage markers. Upon differentiation, cells positive for Alcian blue staining denoted the chondrocyte which shown capacity for further proliferation on Methyl Tetra Toluene assay. Zinc transporter SLC39A14 was expressed on cell membrane. The Collagen 2A produced at a mean level 0.097 ng/ml (SE 0.011;95%CI 0.073-0.12) and Collagen 10A at mean level 31.078 ng/ml (SE 3.792;95%CI 22.870-39.287).

Conclusion: Zinc transporter SLC39A14 was expressed in monolayer human chondrocyte from UC-derived MSC. The chondrocyte shown capacity for proliferation and collagen-producing enables future utility to identify the role of SLC39A14 in chondrocyte pathology.

Keywords: chondrocytes SLC39A14 collagen 2A collagen 10A umbilical cord mesenchymal stromal cell

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How to Cite

Kumara Wati, K. D., Pratiwix, E. D., Dirgantara, Y., Sartika, C. R., Dhamayanti, M., Setiabudiawa, B., & Parwati, I. (2019). Characterizing the SLC39A14 zinc transporter and collagen products of the chondrocytes from the human umbilical cord-derived mesenchymal stromal cell. Bali Medical Journal, 8(2), 672–677. https://doi.org/10.15562/bmj.v8i2.1566
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Ketut Dewi Kumara Wati
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Endah Dianty Pratiwix
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Yanni Dirgantara
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Cynthia Retna Sartika
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Meita Dhamayanti
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Budi Setiabudiawa
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Ida Parwati
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