Background: Secondary hyperuricemia can increase the risk of renal dysfunction in the form of fibrosis and glomerulosclerosis mediated by TGF-Î² signalling. Non-pharmacological therapy through high dietary antioxidant intakes can help in controlling and preventing renal dysfunction by suppressing TGF-Î² expression. This study aimed to analyse the effect of red guava juice (Psidium Guava Linn.) on TGF-Î² protein expression in mice (Mus Musculus) kidneys with secondary hyperuricemia model.
Method: This was an experimental study with a post-test only design. Forty-eight mice (Mus Musculus) were choosen as samples, which were distributed into six different treatment groups. The mice were induced with potassium oxonate for 14 days and given red guava juice for 21 days. TGF-Î² protein expression in mice (Mus Musculus) kidneys was identified through immunohistochemistry using TGF-Î² antibodies. The preparation was observed in the Anatomical Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret using the Olympus XC10 light microscope. Data were statistically analysed using the Kruskal Wallis test, followed by the Post-Hoc Mann Whitney test.
Results: The results of this study showed that the administration of red guava juice had a significant effect on TGF-Î² expression (p = 0.001). There was a significant difference between the negative control treatment and treatment of red guava juice at a dosage of 5 ml/kg/day (p = 0.043).
Conclusion: It can be concluded that the administration of red guava juice in mice (Mus Musculus) with secondary hyperuricemia model can suppress TGF-Î² expression, and therefore, can be used as non-pharmacological therapy.