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Laser acupuncture at BL20 Point Stimulate Pancreatic Beta cell in type 1 diabetes mellitus

  • Abdurachman H. ,
  • Suhariningsih Suhariningsih ,
  • Agus Rubiyanto ,


Abstract

Background: Diabetes mellitus (DM) case continues to rise as WHO reported that the number of adults suffering from DM had almost quadrupled since 1980 to 422 million cases. Laser acupuncture for DM therapy is performed by perpendicularly attaching laser probes to the body, and the laser light was directed to the acupuncture point until reaching the appropriate.

Aim: We aimed to investigate the laser acupuncture at BL20 point in type 1 diabetes mellitus in stimulating pancreatic beta cell.

Methods: Twenty-Two Rattus norvegicus were randomly divided into two groups. Rats in the L1 group were not radiated, while rats in the L2 group were radiated every two days for 12 days. Laser was radiated at BL20 acupuncture point. Fasting blood glucose (FBG) levels were measured in the 13th day by cutting tail. Pancreas tissue ware took to be further microscopically evaluated.

Results: The FBG level and immunohistochemistry of pancreatic beta cell between the two groups were compared using independent t-test and Spearman’s correlation test (p <0.05). Rats in L2 showed a significant decrease in FBG level, a significant increase pancreatic beta cell and Langerhans area.41 patients undergo hysteroscopy procedure dan endometrial biopsy.

Conclusion: This finding indicated that laser acupuncture at BL20 point could reduce FBG level, increase pancreatic beta cell and wider Langerhans area in type 1 diabetes mellitus.

 

Keywords: laser acupuncture BL20 type 1 diabetes mellitus

References

  1. World Health Organization. Global Report on Diabetes. Isbn. 2016;978:88.
  2. Sena CM, Bento CF, Pereira P, Seiça R. Diabetes mellitus: New challenges and innovative therapies. EPMA J. 2010;1:138–63.
  3. Peplow P V., Baxter GD. Electroacupuncture for Control of Blood Glucose in Diabetes: Literature Review. JAMS J. Acupunct. Meridian Stud. Elsevier Korea LLC; 2012;5:1–10.
  4. Al Rashoud AS, Abboud RJ, Wang W, Wigderowitz C. Efficacy of low-level laser therapy applied at acupuncture points in knee osteoarthritis: A randomised double-blind comparative trial. Physiother. (United Kingdom). The Chartered Society of Physiotherapy; 2014;100:242–8.
  5. Abdurachman, Rubiyanto A, Suhariningsih, Hendromartono, Gunawan A. The optimal dose of the low-level laser therapy in the treatment of type 1 diabetes mellitus. Bali Med. J. 2017;7:7–11.
  6. Steven KH, Bossy J, Helms JM, Kang S-K, Kawakita K, Gito P, et al. Guidelines on basic training and safety in acupuncture. Geneva (Switzerland). World Heal. Organ. 1999;
  7. Gao S, Li R, Tian H, Pei E, Cao B, Wu Y. [Effects of electroacupuncture at “Yishu†(EX-B 3) on the relative hormones of HPA axis in rats with type-2 diabetes mellitus]. Zhongguo Zhen Jiu. 2014;34:1099–105.
  8. Realization of real index-guided InGaAlP red lasers with buried tunnel junctions - IEEE Conference P.
  9. Thulesen J, Ørskov C, Holst JJ, Poulsen SS. Short term insulin treatment prevents the diabetogenic action of streptozotocin in rats. Endocrinology. 1997;138:62–8.
  10. Mahay S, Adeghate E, Lindley MZ, Rolph CE, Singh J. Streptozotocin-induced type 1 diabetes mellitus alters the morphology, secretory function and acyl lipid content in the isolated rat parotid salivary gland. Moll Cell Biochem. 2004;
  11. Guz Y, Nasir I, Teitelman G. Regeneration of pancreatic b-cells from intra-islet precursor cells in an experimental model of diabetes. Endocrinology. 2001;142:4956–68.
  12. Al-Shafei AIM, Wise RG, Gresham GA, Carpenter TA, Hall LD, Huang CLH. Magnetic resonance imaging analysis of cardiac cycle events in diabetic rats: the effect of angiotensin-converting enzyme inhibition. J. Physiol. 2002;538:555–72.
  13. Varani J, Perone P, Merfert MG, Moon SE, Larkin D, Stevens MJ. All- trans Retinoic Acid Improves Structure and Function of Diabetic Rat Skin in Organ Culture. 2002;51.
  14. Jang M-H, Shin M-C, Lim B-V, Kim H-B, Kim Y-P, Kim E-H, et al. Acupuncture increases nitric oxide synthase expression in hippocampus of streptozotocin-induced diabetic rats. Am. J. Chin. Med. 2003;31:305–13.
  15. Stewart JA. Manual of Histological Techniques and Their Diagnostic Application. Histopathology. 1995;26:95–95.
  16. Halban PA, Kahn SE, Lernmark A Å, Rhodes CJ. Gene and cell-replacement therapy in the treatment of type 1 diabetes: how high must the standards be set? Diabetes. 2001;50:2181–91.
  17. Marceau K, Ruttle PL, Shirtcliff EA, Essex MJ, Susman EJ, Studies A, et al. The role of transcription factors in the transdifferentiation of pancreatic islet cells. J Mol Endocrinol. 2015. p. 742–68.
  18. Oi K, Komori H, Kajinuma H. Changes in plasma glucose, insulin, glucagon, catecholamine, and glycogen contents in tissues during development of alloxan diabetes mellitus in rats. Biochem. Mol. Med. 1997;62:70–5.
  19. Eisenbarth G. S. Type 1 diabetes: molecular, cellular and clinical immunology. Adv Exp Med Biol. 2004;552:306–10.
  20. Gu G, Brown JR, Melton DA. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech. Dev. 2003;120:35–43.
  21. Zulewski H, Abraham EJ, Gerlach MJ, Daniel PB, Moritz W, Müller B, et al. Multipotent nestin-positive stem cells isolated from adult pancreatic islets differenciate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes. Diabetes. 2001;50:521–33.
  22. Dor Y, Melton DA. Pancreatic Stem Cells. Handb. Stem Cells. 2004;2:513–20.
  23. Bonner-Weir S, Toschi E, Inada A, Reitz P, Fonseca SY, Aye T, et al. The pancreatic ductal epithelium serves as a potential pool of progenitor cells. Pediatr. Diabetes. 2004;5 Suppl 2:16–22.
  24. Fehsel K, Kolb-Bachofen V, Kröncke K-D. Necrosis is the predominant type of islet cell death during development of insulin-dependent diabetes mellitus in BB rats. Lab. Invest. 2003;83:549–59.
  25. Sadler A, Thomas TWW. Langman’s medical embryology. 12th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins; 2012.
  27. World Health Organization. Global Report on Diabetes. Isbn. 2016;978:88.
  28. Sena CM, Bento CF, Pereira P, Seiça R. Diabetes mellitus: New challenges and innovative therapies. EPMA J. 2010;1:138–63.
  29. Peplow P V., Baxter GD. Electroacupuncture for Control of Blood Glucose in Diabetes: Literature Review. JAMS J. Acupunct. Meridian Stud. Elsevier Korea LLC; 2012;5:1–10.
  30. Al Rashoud AS, Abboud RJ, Wang W, Wigderowitz C. Efficacy of low-level laser therapy applied at acupuncture points in knee osteoarthritis: A randomised double-blind comparative trial. Physiother. (United Kingdom). The Chartered Society of Physiotherapy; 2014;100:242–8.
  31. Abdurachman, Rubiyanto A, Suhariningsih, Hendromartono, Gunawan A. The optimal dose of the low-level laser therapy in the treatment of type 1 diabetes mellitus. Bali Med. J. 2017;7:7–11.
  32. Steven KH, Bossy J, Helms JM, Kang S-K, Kawakita K, Gito P, et al. Guidelines on basic training and safety in acupuncture. Geneva (Switzerland). World Heal. Organ. 1999;
  33. Gao S, Li R, Tian H, Pei E, Cao B, Wu Y. [Effects of electroacupuncture at “Yishu†(EX-B 3) on the relative hormones of HPA axis in rats with type-2 diabetes mellitus]. Zhongguo Zhen Jiu. 2014;34:1099–105.
  34. Realization of real index-guided InGaAlP red lasers with buried tunnel junctions - IEEE Conference P.
  35. Thulesen J, Ørskov C, Holst JJ, Poulsen SS. Short term insulin treatment prevents the diabetogenic action of streptozotocin in rats. Endocrinology. 1997;138:62–8.
  36. Mahay S, Adeghate E, Lindley MZ, Rolph CE, Singh J. Streptozotocin-induced type 1 diabetes mellitus alters the morphology, secretory function and acyl lipid content in the isolated rat parotid salivary gland. Moll Cell Biochem. 2004;
  37. Guz Y, Nasir I, Teitelman G. Regeneration of pancreatic b-cells from intra-islet precursor cells in an experimental model of diabetes. Endocrinology. 2001;142:4956–68.
  38. Al-Shafei AIM, Wise RG, Gresham GA, Carpenter TA, Hall LD, Huang CLH. Magnetic resonance imaging analysis of cardiac cycle events in diabetic rats: the effect of angiotensin-converting enzyme inhibition. J. Physiol. 2002;538:555–72.
  39. Varani J, Perone P, Merfert MG, Moon SE, Larkin D, Stevens MJ. All- trans Retinoic Acid Improves Structure and Function of Diabetic Rat Skin in Organ Culture. 2002;51.
  40. Jang M-H, Shin M-C, Lim B-V, Kim H-B, Kim Y-P, Kim E-H, et al. Acupuncture increases nitric oxide synthase expression in hippocampus of streptozotocin-induced diabetic rats. Am. J. Chin. Med. 2003;31:305–13.
  41. Stewart JA. Manual of Histological Techniques and Their Diagnostic Application. Histopathology. 1995;26:95–95.
  42. Halban PA, Kahn SE, Lernmark A Å, Rhodes CJ. Gene and cell-replacement therapy in the treatment of type 1 diabetes: how high must the standards be set? Diabetes. 2001;50:2181–91.
  43. Marceau K, Ruttle PL, Shirtcliff EA, Essex MJ, Susman EJ, Studies A, et al. The role of transcription factors in the transdifferentiation of pancreatic islet cells. J Mol Endocrinol. 2015. p. 742–68.
  44. Oi K, Komori H, Kajinuma H. Changes in plasma glucose, insulin, glucagon, catecholamine, and glycogen contents in tissues during development of alloxan diabetes mellitus in rats. Biochem. Mol. Med. 1997;62:70–5.
  45. Eisenbarth G. S. Type 1 diabetes: molecular, cellular and clinical immunology. Adv Exp Med Biol. 2004;552:306–10.
  46. Gu G, Brown JR, Melton DA. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech. Dev. 2003;120:35–43.
  47. Zulewski H, Abraham EJ, Gerlach MJ, Daniel PB, Moritz W, Müller B, et al. Multipotent nestin-positive stem cells isolated from adult pancreatic islets differenciate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes. Diabetes. 2001;50:521–33.
  48. Dor Y, Melton DA. Pancreatic Stem Cells. Handb. Stem Cells. 2004;2:513–20.
  49. Bonner-Weir S, Toschi E, Inada A, Reitz P, Fonseca SY, Aye T, et al. The pancreatic ductal epithelium serves as a potential pool of progenitor cells. Pediatr. Diabetes. 2004;5 Suppl 2:16–22.
  50. Fehsel K, Kolb-Bachofen V, Kröncke K-D. Necrosis is the predominant type of islet cell death during development of insulin-dependent diabetes mellitus in BB rats. Lab. Invest. 2003;83:549–59.
  51. Sadler A, Thomas TWW. Langman’s medical embryology. 12th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins; 2012.

How to Cite

H., A., Suhariningsih, S., & Rubiyanto, A. (2019). Laser acupuncture at BL20 Point Stimulate Pancreatic Beta cell in type 1 diabetes mellitus. Bali Medical Journal, 8(1), 241–246. https://doi.org/10.15562/bmj.v8i1.1436
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Abdurachman H.
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Suhariningsih Suhariningsih
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Agus Rubiyanto
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