Hyperbaric Oxygen Therapy (HBOT) is a method of increasing oxygen delivery to tissues. The therapy improves tissue oxygenation and stimulates the formation of H2O2 as a secondary messenger for Tumour Necrosis Factor alpha (TNFÎ±), Interferon Gamma (IFNÎ³) and Nuclear Factor Kappa Beta phosphorylation (NF-Kb) which play an important role in the rapid transcription of a wide variety of genes in response to extracellular stimuli. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of IFNÎ³ and TNFÎ± in pregnant rats infected with Toxoplasma gondii.
To find effect of Hyperbaric Oxygen Therapy in enhancing the expressions of IFNÎ³ and TNFÎ± pregnant Rattus n
overgiccus infected with Toxoplasma gondii.
Patients and methods:
This study is an animal study with a â€˜randomized control group of post test only designâ€™ on 34 Rattus novergiccus Sprague Dowley rats. Randomly, the rats were divided into 4 groups. The Hyperbaric Oxygen Therapy (HBOT) treatment group A is pregnant rats infected with tachyzoite received 10 sessions of HBOT 2.4 ATA in 3x30 minutes; the HBOT treatment group B is Pregnant only and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes; the HBOT treatment group C is Pregnant and infected with tachyzoite but not received HBOT; and the group D is pregnant rats only without infection and not received HBOT. Each infected pregnant rats were given a 103 Tachyzoite of Toxoplasma gondiiÂ via intraperitoneal injection. Examinations of IFNÎ³ and TNFÎ± expressions were performed on day-5 after HBOT (HBOT twice a day). Rats that die or experience abortion will be eliminated while rats that still survive will be taken the blood by intracardiac technique. IFNÎ³ and TNFÎ± levels were measured by serum ELISA examination.
The results shows that the Hyperbaric Oxygen Therapy can improve IFNÎ³ (p=0.000), TNF-Î± (p= 0.02) significantly in the provision of HBOT 2.4 ATA for 3x30 minutes in 10 sessions over 5 days of therapy.
Based on the obtaine results,it can be conclude that HBOT can improve the expressions of IFNÎ³ and TNFÎ±, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 day and HBOT administration can prevent abortion in pregnant rats infected with tachyzoite T. gondii.
Keywords: [Toxoplasma gondii, Tachyzoite, Hyperbaric Oxygen Therapy, IFNÎ³, TNFÎ±]Fundings: -