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The effect of probiotic Lactobacillus casei supplementation on the secretory immunoglobulin A level in the saliva of wistar rats


Introduction: Secretory immunoglobulin A (SIgA) is an antibody that plays an important role in mucosal immunity by blockading epithelial receptors inhibiting the attachment of pathogenic bacteria, especially Streptococcus mutans to epithelial cells. Lactobacillus is one of the bacteria contained in probiotics, which can increase the proliferation of SIgA-producing cells. This study aims to determine the effect of probiotic Lactobacillus casei supplementation on increasing SIgA levels in saliva of wistar rats.

Methods: This study used a laboratory prospective experimental research design, with a simple randomized design in which thirty-six male wistar rats were adapted for 7 days. After adaptation, the experimental animals that fulfilled the inclusion criteria were divided into 2 groups randomly, i.e., the treatment group who received probiotic supplement Lactobacillus casei 1.17ml / 200gBW twice daily, and the control group receiving only standard water and food, the intervention was performed for 14 days. Examination of SIgA levels in saliva was conducted with ELISA.

Results: Based on Mann-Whitney analysis, the results showed significant differences in SIgA levels between treatment and control groups with median SIgA values of 26.668 ng /ml and 4,463 ng/ml, with p values being 0.033 (p <0.05).

Conclusion: Probiotic supplementation of Lactobacillus casei could increase SIgA levels in saliva of wistar rats.


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How to Cite

Hayati, M., Herman, H., & Rezano, A. (2018). The effect of probiotic Lactobacillus casei supplementation on the secretory immunoglobulin A level in the saliva of wistar rats. Bali Medical Journal, 7(3).




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