Skip to main content Skip to main navigation menu Skip to site footer

Optimising the effect of activated carbon on the microrheological properties of erythrocyte in rats with experimentally developed obesity



Abstract

Background: Remains not fully clear the impact of intestinal chelators on microrheological violation of red blood cells during the early stages of obesity. It dictates the necessity of experimental investigations' fulfilment on laboratory animals with just developed obesity in them.

Method: Ninety-four of healthy male-rats of Vistar line at the age of 2.5-3 months took into investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. In 65 rats after their putting into small cages (one specimen - in a cage) during 30 days there was developed OB as the result of giving them of high-caloric diet from combined feed (47%), sweet condensed milk (44%), vegetable oil (8%) and vegetable starch (1%). Of the 35 rats received a day for 60 days of activated carbon 25 mg/kg into a stomach. There used biochemical, haematological and statistical methods of investigation.

Results: At obesity development in rats, there found a steady decrease of erythrocytes-discocytes quantity in blood. It accompanied by increase of reversibly and irreversibly changed erythrocytes' quantity in examined animals' blood. At obesity development in rats there was found quick rise of erythrocytes' sum in aggregate and these aggregates' quantity at lowering of free erythrocytes' number. During experimental obesity modelling we noticed very early in rats' blood decrease of erythrocytes-discocytes' quantity, level rise of their reversibly and irreversibly varieties with strengthening of their aggregative ability. It takes place in the background of weakening of erythrocytes' antioxidant protection and activation of lipids' peroxidation in them.

Conclusion: Application of activated carbons capable of rats with experimental obesity resolve arisen they breach microrheological properties of erythrocytes. It is clear that in obesity treatment regimens must be intestinal chelators, capable of positively affect many broken with this pathology haematological indices.

Keywords: rats erythrocytes microrheological features experiment obesity activated charcoal

References

  1. Kulikov EV, Seleznev SB, Sotnikova ED, Vatnikov YuA, Kharlitskaya EV, Parshina VI, Rystsova EO, and Troshina NI. The Morphological Aspects of Bone Marrow of Guinea Fowl of the Volga White Breed in Postembryonic Ontogenesis. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2016; 7(5) : 1148-1153.
  2. Kulikov EV, Vatnikov YA, Parshina VI, Sotnikova ED, Vilkovyskiy IF, Popova IA, Kochneva MV, Karamyan AS. Special aspects of the pathohistological diagnostics of familial shar-pei amyloidosis. Asian Journal of Pharmaceutics. 2017; 11 (1) : 152-157.
  3. Lenchenko EM, Vatnikov YA, Sotnikova ED, Kulikov EV, Gnezdilova LA, Seleznev SB, Strizhakov AA, Kuznetsov VI. Experimental toxaemia of chickens contaminated with Yersinia enterocolitica bacteria. Asian Journal of Pharmaceutics. 2017; 11(1) : 91-96.
  4. Seleznev SB, Kulikov EV, Vetoshkina GA, Vatnikov YA, Sotnikova ED, Krotova EA, Yagnikov SA, Yakunina MN. The evolution and structural organization of the organs of vertebrate immune system. Asian Journal of Pharmaceutics. 2017; 11(1) : 84-90.
  5. Karamyan AS, Vatnikov YuA, Savochkina AYu. The effect of inorganic selenium on the quality of minks’ coat under the cage housing. Journal of Trace Elements in Medicine and Biology. 2017; 41: 66
  6. Byakhova VM, Vatnikov YuA. Iodine-induced diseases in birds. Journal of Trace Elements in Medicine and Biology. 2017; 41 : 84.
  7. Medvedev IN, Gromnatskii NI, Golikov BM, Al'-Zuraiki EM, Li VI. Effects of lisinopril on platelet aggregation in patients with arterial hypertension with metabolic syndrome. Kardiologiia. 2004; 44(10) : 57–59.
  8. Medvedev IN, Gromnatskii NI, Mokhamed A.-ZE. Comparative Assessment of Effects of Qadropril and Enalapril on Intravascular Activity of Platelets in Hypertensive Patients With Metabolic Syndrome. Kardiologiia. 2004; 44(12):44–46.
  9. Wandersee NJ, Punzalan RC, Rettig MP. Erythrocyte adhesion is modified by alterations in cellular tonicity and volume. Brit. J. Haematol. 2005;3:366–377.
  10. Medvedev IN, Skoriatina IA. Dynamics of microrheologic properties of erythrocytes in patients with arterial hypertension and dyslipidemia treated with atorvastatin. Klinicheskaia meditsina. 2012;90(6):42–45.
  11. Medvedev IN, Skoryatina IA. Fluvastatin effects on blood cell aggregation in patients with arterial hypertension and dyslipidemia. Cardiovascular Therapy and Prevention. 2013; 12(2) : 18-24.
  12. Medvedev IN, Skoriatina IA. Effect of lovastatin on adhesive and aggregation function of platelets in patients with arterial hypertension and dyslipidemia. Klinicheskaia meditsina. 2010;88(2):38–40.
  13. Medvedev IN, Skoryatina IA. Platelet hemostasis dynamics in simvastatin- treated patients with arterial hypertension and dyslipidemia. Russian Journal of Cardiology. 2010;1(81): 54–58.
  14. Medvedev IN, Skoryatina IA. Aggregation properties of blood cells and vascular control over them in patients with arterial hypertension and dyslipidemia. Russian Journal of Cardiology. 2015; 4(120) : 18-22.
  15. Amelina IV, Medvedev IN. Transcriptional activity of chromosome nucleolar organizing regions in population of Kursk region. Bulletin of Experimental Biology and Medicine. 2009;147(6):730–732.
  16. Medvedev IN, Amelina IV. An association between human morphological phenotypical characteristics and the activity of chromosomal nucleolar organizer regions in the interphase cell nucleus in the population of indigenous people of Kursk region. Morfology. 2012;142(4):87–91.
  17. Simonenko V.В., Medvedev I. N, Nosova T. Yu. Aggreation function of platelets in persons with arterial hypertension and abdominal obesity. Klinicheskaia meditsina. 2008; 86(5):22–24.
  18. Simonenko VB, Medvedev IN, Tolmachev VV. Pathogenetic aspects of arterial hypertension in metabolic syndrome Klinicheskaia meditsina. 2011; 89(1):49–51.
  19. Medvedev IN, Gromnatskii NI. Correction of thrombocyte hemostasis and biological age reduction in metabolic syndrome. Klinicheskaia meditsina. 2005;83(8):54–57.
  20. Medvedev IN, Gromnatskii NI, Volobuev IV, Dement'ev VI, Storozhenko MV. Thrombocytic hemostasis in hypertensive patients with metabolic syndrome and its correction with lovastatin. Klinicheskaia meditsina. 2004;82(10):37–41.
  21. Medvedev IN, Skoryatina IA. The aggregation capacity of neutrophils in patients with arterial hypertension and dyslipidemia treated with fluvastatin. Klinicheskaia meditsina. 2015; 93(1):66–70.
  22. Medvedev IN, Skoryatina IA. Erythrocyte aggregation in patients with arterial hypertension and dyslipidemia treated with pravastatin. Klinicheskaia meditsina. 2014; 92(11):34–38.
  23. Medvedev IN, Skoryatina IA. Pravastatin in correction of vessel wall antiplatelet control over the blood cells in patients with arterial hypertension and dyslipidemia. Cardiovascular therary and prevention. 2014; 13(6) : 18-22.
  24. Medvedev IN, Gromnatskii NI, Volobuev IV, Osipova VM, Storozhenko MV. Correction of thrombocyte-vascular hemostasis in metabolic syndrome. Klinicheskaia meditsina. 2006;84(1):46–49.
  25. Medvedev IN, Skoryatina IA. Fluvastatin effects on blood cell aggregation in patients with arterial hypertension and dyslipidemia. Cardiovascular Therapy and Prevention. 2013; 12(2):18–24.
  26. Kutafina NV, Medvedev IN. Platelet aggregation clinically healthy persons of the second coming of age living in the Kursk region. Advances in gerontology = Uspekhi gerontologii / Rossiiskaia akademiia nauk, Gerontologicheskoe obshchestvo. 2015;28(2):321–325.
  27. Medvedev IN. Dynamics of violations of intravascular platelet activity in rats during the formation of metabolic syndrome using fructose models. Problems of nutrition. 2016; 85(1):42–46.
  28. Medvedev IN, Gamolina OV. Lisinopril effects on platelet activity in patients with arterial hypertension and impaired glucose tolerance. Russian Journal of Cardiology. 2008; 3 : 45-48.
  29. Medvedev IN, Gromnatskii NI. The influence of hypocaloric diet on thrombocyte rheology in patients with metabolic syndrome. Klinicheskaia meditsina. 2006;84(3):49–52.
  30. Gromnatskii NI, Medvedev IN. Non-pharmacological correction of impaired platelet hemostasis in hypertensive patients with metabolic syndrome. Klinicheskaia meditsina. 2003;81(4):31–34.
  31. Medvedev IN, Gromnatskii NI. Normodipin in correction of platelet rheology in hypertensive patients with metabolic syndrome. Terapevticheskii Arkhiv. 2005; 77(6) : 65-68.
  32. Medvedev IN, Danilenko OA. Comparative effects of therapeutic complexes on vascular wall activity in patients with arterial hypertension, metabolic syndrome, and recent ocular vessel occlusion. Cardiovascular therapy and prevention. 2010; 9(7):27–32.
  33. Medvedev IN, Danilenko OA. Complex correction of vascular hemostasis in patients with arterial hypertension, metabolic syndrome, and recent ocular vessel occlusion. Russian Journal of Cardiology. 2010; 4(84):15–19.
  34. Medvedev IN, Danilenko OA. Effectiveness of vascular wall activity correction in patients with arterial hypertension, metabolic syndrome, and oculo-vascular occlusion. Russian Journal of Cardiology. 2010; 3(83):64–67.
  35. Medvedev IN. Platelet functional activity in rats, prolonged experiencing regular exercise. Vestnik SPbSU. Series 3. Biology. 2016;4:99–107. DOI: 10.21638/11701/spbu03.2016.407.
  36. Medvedev IN, Savchenko AP. Platelet activity correction by regular physical training in young people with high normal blood pressure. Russian Journal of Cardiology. 2010; 2(82):35–40.
  37. Bikbulatova AA, Karplyuk AA, Tarasenko OV. Model of Activities of the Resource Training Center of the Russian State Social University in Terms of Professional Orientation and Employment of Persons with Disabilities. Psikhologicheskaya nauka i obrazovanie. 2017; 22(1): 26-33.
  38. Bikbulatova AA, Pochinok NB. Professional Skills Competitions for People with Disabilities as a Mechanism for Career Guidance and Promotion of Employment in People with Special Needs. Psikhologicheskaya nauka i obrazovanie. 2017; 22(1) : 81-87.
  39. Amelina IV, Medvedev IN. Relationship between the chromosome nucleoli-forming regions and somatometric parameters in humans. Bulletin of Experimental Biology and Medicine. 2009; 147(1) : 77-80.
  40. Chevari S, Andyal T, Strenger J. Determination of antioxidant blood parameters and their diagnostic value in the elderly. Laboratory work. 1991;10:9–13.
  41. Volchegorskiy IA Dolgushin II, Kolesnikov OL, Tseilikman VE. Experimental modeling and laboratory evaluation of adaptive reactions of the organism. Chelyabinsk, 2000:167.
  42. Medvedev IN, Savchenko AP, Zavalishina SYu, Krasnova EG, Kumova TA, Gamolina OV, Skoryatina IA, Fadeeva TS. Methodological approaches to the study of the rheological properties of blood in various states. Russian Journal of Cardiology. 2009;5: 42–45.
  43. Medvedev IN, Kumova TA. Angiotensin II receptor inhibitors: role and place in arterial hypertension and metabolic syndrome treatment. Russian Journal of Cardiology. 2007; 5 : 97-99.
  44. Medvedev IN, Amelina IV. Evaluation of the relationship between chromosome aberrations and transcription activity of nucleolus organizer regions in indigenous Population of the Kursk Region. Bulletin of Experimental Biology and Medicine. 2010; 149(3):332–336.
  45. Medvedev IN, Amelina IV. AG polymorphism as a cytogenetic maker of arterial hypertension risk. Russian Journal of Cardiology. 2009;2(76):70–72.
  46. Amelina IV, Medvedev IN. Evaluation of the dependence of mutagenesis intensity on activity of nucleolus organizer regions of chromosomes in aboriginal population of Kursk region. Bulletin of Experimental Biology and Medicine. 2008; 145(1):68–71.
  47. Simonenko VB, Medvedev IN, Kumova TA. Pathogenetic aspects of hypertension in case of metabolic syndrome. Voenno-meditsinskiÇ zhurnal. 2010; 331(9):41–44.
  48. Simonenko VB, Medvedev IN, Mezentseva NI, Tolmachev VV. The antiaggregation activity of the vascular wall in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina. 2007;85(7):28–30.
  49. Simonenko VB, Medvedev IN, Tolmachev VV. Dynamics of primary hemostasis activity in patients with arterial hypertension and metabolic syndrome treated with candesartan. Klinicheskaia meditsina. 2011;89(3):35–38.
  50. Medvedev IN, Gromnatskii NI. The influence of nebivolol on thrombocyte aggregation in patients with arterial hypertension with metabolic syndrome. Klinicheskaia meditsina. 2005;83(3):31–33.
  51. Simonenko VB, Medvedev IN, Gamolina OV. Primary hemostasis activity in patients with arterial hypertension and impaired glucose tolerance treated with trandolapril. Klinicheskaia meditsina. 2011;89(2):29–31.
  52. Simonenko VB, Medvedev IN, Tolmachev VV. Comparative evaluation of the influence of sulfhydryl and phosphate ACE inhibitors on thrombocyte aggregation in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina. 2007;85(4) : 24–27.
  53. Medvedev IN, Gromnatskii NI. Effect of amlodipine on intravascular thrombocyte activity in patients with arterial hypertension and metabolic syndrome Klinicheskaia meditsina. 2005;83(2):37–39.
  54. Simonenko VB, Medvedev IN, Tolmachev VV. Effect of irbesartan on the function of hemocoagulative component of hemostasis in patients with arterial hypertension during metabolic syndrome. Klinicheskaia meditsina. 2010;88(6):27–30.
  55. Medvedev IN, Nosova TYu. Verospiron effects on platelet aggregation in patients with arterial hypertension and abdominal obesity. Russian Journal of Cardiology. 2007; 6 : 55-58.
  56. Medvedev IN, Kumova TA, Gamolina OV. Renin-angiotensis system role in arterial hypertension development. Russian Journal of Cardiology. 2009;4:82–84.
  57. Medvedev IN, Kumova TA. Eprosartan effects on intravascular platelet activity in patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology. 2008; 1(69) : 40-42.
  58. Medvedev IN. A comparative analysis of normodipin and spirapril effects on intravascular activity of platelets in patients with metabolic syndrome. Terapevticheskii Arkhiv. 2007; 79(10):25–27.
  59. Medvedev IN, Kumova TA. Reduced platelet aggregation in losartan-treated patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology. 2008; 5: 53–55.
  60. Medvedev IN, Skoryatina IA. Platelet hemostasis dynamics in simvastatin- treated patients with arterial hypertension and dyslipidemia. Russian Journal of Cardiology. 2010; 1(81): 54–58.
  61. Medvedev IN, Mezentseva IN, Tolmachev VV. ACE inhibitors potential in correcting vessel wall anti-aggregation activity among patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology. 2007;1:48–52.
  62. Medvedev IN, Plotnikov AV, Kumova TA. Rapid normalization of platelet hemostasis in patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology. 2008;2:43–46.
  63. Medvedev IN. Correction of primary hemostasis in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina. 2007;85(3):29–33.
  64. Simonenko VB, Medvedev IN, Kumova TA. Losartan for correction of thrombocyte activity in patients suffering from arterial hypertension with metabolic syndrome. Klinicheskaia meditsina. 2008;86(1):38–41.
  65. Medvedev IN, Kumova TA. Comparison of platelet hemostasis effects for angiotensin receptor blockers in patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology. 2007;4:52–56.
  66. Medvedev IN, Kumova TA. Valsartan effects on platelet activity in patients with arterial hypertension and metabolic syndrome. Russian Journal of Cardiology. 2007;3:66-69.

How to Cite

Kulikov, E. V., Zavalishina, S. Y., Vatnikov, Y. A., Strizhakov, A. A., Drukovsky, S. G., Lozovoy, D. A., Voronina, Y. Y., Popova, I. A., Bondareva, I. V., Sambros, N. B., Norezzine, A., Glagoleva, T. I., & Shemyakova, S. A. (2019). Optimising the effect of activated carbon on the microrheological properties of erythrocyte in rats with experimentally developed obesity. Bali Medical Journal, 8(3), 827–833. https://doi.org/10.15562/bmj.v8i3.1112
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
0

Total
9

Share

Search Panel

Evgeniy Vladimirovich Kulikov
Google Scholar
Pubmed
BMJ Journal

Svetlana Yurevna Zavalishina
Google Scholar
Pubmed
BMJ Journal

Yuri Anatolyevich Vatnikov
Google Scholar
Pubmed
BMJ Journal

Alexander Anatolyevich Strizhakov
Google Scholar
Pubmed
BMJ Journal

Stanislav Gennadievich Drukovsky
Google Scholar
Pubmed
BMJ Journal

Dmitry Anatolyevich Lozovoy
Google Scholar
Pubmed
BMJ Journal

Yuliya Yurevna Voronina
Google Scholar
Pubmed
BMJ Journal

Irina Anatolyevna Popova
Google Scholar
Pubmed
BMJ Journal

Irina Viktorovna Bondareva
Google Scholar
Pubmed
BMJ Journal

Natalia Borisovna Sambros
Google Scholar
Pubmed
BMJ Journal

Abdelaziz Norezzine
Google Scholar
Pubmed
BMJ Journal

Tatyana Ivanovna Glagoleva
Google Scholar
Pubmed
BMJ Journal

Svetlana Alexandrovna Shemyakova
Google Scholar
Pubmed
BMJ Journal